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Azoarcus taiwanensis sp. nov., a denitrifying species isolated from a hot spring

  • Environmental biotechnology
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Abstract

The strain NSC3T, a novel, facultative, chemolithotrophic, denitrifying, alkaliphilic, sulfide-oxidizing bacterium isolated from a hot spring in Yang-Ming Mountain, Taiwan, was Gram negative, rod shaped, and motile by single polar flagella and grew facultatively by adopting a denitrifying metabolism. The 16S rRNA sequence analysis revealed that strain NSC3T belongs to beta subclass of the Proteobacteria and most closely related to Azoarcus evansii KB740T (95.44 %), Azoarcus toluvorans Td-21T (95.21 %), Azoarcus tolulyticus Tol-4T (95.08 %), and Azoarcus toluclasticus MF63T (94.94 %). The phylogenetic analyses based on 16S rRNA gene sequences indicated that the strain NSC3T formed a distinct lineage in the Betaproteobacteria and that it exhibited the highest level of sequence similarity with species of the genera Azoarcus (95.28–93.13 %). The major fatty acids of the type strain were C16:0 (26.9 %), C16:1w7c (28.9 %), C18:0 (9.6 %), and C18:1w7c/w6c (29.9 %). The DNA G+C content of genomic DNA was 63.7 mol%. On the basis of the 16S rRNA sequence similarity, phenotypic and genotypic characteristics, and chemotaxonomic data, the strain NSC3T could be differentiated from other species of the genus Azoarcus. Therefore, strain NSC3T (equal to BCRC 80111T and DSM 24109T) is proposed as a novel species in genus Azoarcus, for which the name Azoarcus taiwanensis sp. nov. is proposed. The strain NSC3T is deposited in Bioresource Collection and Research Center, Taiwan, under the reference number BCRC 80111T, and German Collection of Microorganisms and Cell Cultures, Germany (DSMZ), with DSM 24109T.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan, 106

    Duu-Jong Lee, Biing-Teo Wong & Sunil S. Adav

  2. Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan, 106

    Duu-Jong Lee

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  1. Duu-Jong Lee
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  2. Biing-Teo Wong
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  3. Sunil S. Adav
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Correspondence to Duu-Jong Lee.

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Lee, DJ., Wong, BT. & Adav, S.S. Azoarcus taiwanensis sp. nov., a denitrifying species isolated from a hot spring. Appl Microbiol Biotechnol 98, 1301–1307 (2014). https://doi.org/10.1007/s00253-013-4976-9

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