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Using 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. MSH1 in flow through biofilters—initial adhesion and BAM degradation potentials

  • Environmental biotechnology
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Abstract

Micropollutants in groundwater are given significant attention by water companies and authorities due to an increasing awareness that they might be present even above the legal threshold values. As part of our investigations of the possibility to remove the common groundwater pollutant 2,6-dichlorobenzamide (BAM) by introducing the efficient BAM degrader Aminobacter sp. MSH1 into biologically active sand filters, we investigated if the strain adheres to filters containing various filter materials and if the initial adherence and subsequent degradation of BAM could be optimized. We found that most of the inoculated MSH1 cells adhered fast and that parameters like pH and ionic strength had only a minor influence on the adhesion despite huge influence on cell surface hydrophobicity. At the given growth protocol, the MSH1 strain apparently developed a subpopulation that had lost its ability to adhere to the filter materials, which was supported by attempted reinoculation of non-adhered cells. Analysis by quantitative PCR showed that most cells adhered in the top of the filters and that some of these were lost from the filters during initial operation, while insignificant losses occurred after 1 day of operation. The inoculated filters were found to degrade 2.7 μg/L BAM to below 0.1 μg/L at a 1.1-h residence time with insignificant formation of known degradation products. In conclusion, most filter materials and water types should be feasible for inoculation with the MSH1 strain, while more research into degradation at low concentrations and temperatures is needed before this technology is ready for use at actual waterworks.

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References

  • Ahammed MM, Meera V (2010) Metal oxide/hydroxide-coated dual-media filter for simultaneous removal of bacteria and heavy metals from natural waters. J Haz Mat 181:788–793

    Article  CAS  Google Scholar 

  • Arvin E, Nielsen L, Tully A, Albrechtsen H-J, Mosbæk H (2004) MTBE removal by biofiltration in a water works. In: Van Loosdrecht M, Clement J (eds) 2nd IWA leading-edge conference on water and wastewater treatment technologies. IWA, London, pp 120–121

    Google Scholar 

  • Baumgarten T, Sperling S, Seifert J, Von Bergen M, Steiniger F, Wick LY, Heipieper HJ (2012) Membrane vesicle formation as a multiple-stress response mechanism enhances Pseudomonas putida DOT-T1E cell surface hydrophobicity and biofilm formation. Appl Environ Microbiol 78:6217–6224

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Bolster CH, Mills AL, Hornberger GM, Herman JS (1999) Spatial distribution of deposited bacteria following Miscible Displacement Experiments in intact cores. Wat Resour Res 35:1797–1807

    Article  CAS  Google Scholar 

  • Bolster CH, Cook KL, Marcus IM, Haznedaroglu BZ, Walker SL (2010) Correlating transport behavior with cell properties for eight porcine Escherichia coli isolates. Environ Sci Technol 44:5008–5014

    Article  CAS  PubMed  Google Scholar 

  • DeFlaun M, Murray C, Holben W, Scheibe T, Mills A, Ginn T, Griffin T, Majer E, Wilson J (1997) Preliminary observations on bacterial transport in a coastal plain aquifer. FEMS Microbiol Rev 20:473–487

    Article  CAS  Google Scholar 

  • Gregg SJ, Sing KSW (1991) Adsorption, surface area, and porosity, 2nd edn. Academic, London

    Google Scholar 

  • Harms H, Zehnder AJ (1994) Influence of substrate diffusion on degradation of dibenzofuran and 3-chlorodibenzofuran by attached and suspended bacteria. Appl Environ Microbiol 60:2736–2745

    CAS  PubMed Central  PubMed  Google Scholar 

  • Harvey RW, Harms H (2002) Transport of microorganisms in the terrestrial subsurface: in situ and laboratory methods. In: Hurst CJ, Knudsen GR, McInerney MJ, Stetzenbach LD, Crawford R (eds) Manual of environmental microbiology, 2nd edn. ASM, Washington, DC, pp 753–776

    Google Scholar 

  • Heise S, Gust G (1999) Influence of the physiological status of bacteria on their transport into permeable sediments. Mar Ecol Prog Ser 190:141–153

    Article  Google Scholar 

  • Ho L, Meyn T, Keegan A, Hoefel D, Brookes J, Saint CP, Newcombe G (2006) Bacterial degradation of microcystin toxins within a biologically active sand filter. Wat Res 40:768–774

    Article  CAS  Google Scholar 

  • Kinoshita T, Bales RC, Yahya MT, Gerba CP (1993) Bacteria transport in a porous medium: retention of Bacillus and Pseudomonas on silica surfaces. Wat Res 27:1295–1301

    Article  Google Scholar 

  • Kosmulski M (2009) Surface charging and points of zero charge. CRC, Boca Raton

    Book  Google Scholar 

  • Lee C-G, Park S-J, Han Y-U, Park J-A, Kim S-B (2010) Bacterial attachment and detachment in aluminum-coated quartz sand in response to ionic strength change. Wat Environ Res 82:499–505

    Article  CAS  Google Scholar 

  • Li B, Logan BE (2004) Bacterial adhesion to glass and metal-oxide surfaces. Coll Surf B Biointerfaces 36:81–90

    Article  CAS  Google Scholar 

  • Manem J, Rittmann B (1992) Removing trace-level organic pollutants in a biological filter. J AWWA 84:152–157

    CAS  Google Scholar 

  • McDowall B, Hoefel D, Newcombe G, Saint CP, Ho L (2009) Enhancing the biofiltration of geosmin by seeding sand filter columns with a consortium of geosmin-degrading bacteria. Wat Res 43:433–440

    Article  CAS  Google Scholar 

  • Meffe R, Kohfahl C, Holzbecher E, Massmann G, Richter D, Dünnbier U, Pekdeger A (2010) Modelling the removal of p-TSA (para-toluenesulfonamide) during rapid sand filtration used for drinking water treatment. Wat Res 44:205–213

    Article  CAS  Google Scholar 

  • Mills AL, Herman JS, Hornberger GM, DeJesus TH (1994) Effect of solution ionic strength and iron coatings on mineral grains on the sorption of bacterial cells to quartz sand. Appl Environ Microbiol 60:3300–3306

    CAS  PubMed Central  PubMed  Google Scholar 

  • Park S-J, Kim S-B (2010) Influence of (bi)carbonate on bacterial interaction with quartz and metal oxide-coated surfaces. Coll Surf B Biointerfaces 76:57–62

    Article  CAS  Google Scholar 

  • Park SJ, Lee C-G, Kim SB (2008) Quantification of bacterial attachment-related parameters in porous media. Environ Eng Res 13:141–146

    Article  Google Scholar 

  • Rijnaarts HHM, Norde W, Bouwer EJ, Lyklema J, Zehnder AJB (1996) Bacterial deposition in porous media: effects of cell-coating, substratum hydrophobicity, and electrolyte concentration. Environ Sci Technol 30:2877–2883

    Article  CAS  Google Scholar 

  • Rosenberg M, Gutnick D, Rosenberg E (1980) Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity. FEMS Microbiol Lett 9:29–33

    Article  CAS  Google Scholar 

  • Simoni SF, Harms H, Bosma TNP, Zehnder AJB (1998) Transport of bacteria through sand columns at low flow rates. Environ Sci Technol 32:2100–2105

    Article  CAS  Google Scholar 

  • Simoni SF, Schäfer A, Harms H, Zehnder AJ (2001) Factors affecting mass transfer limited biodegradation in saturated porous media. J Cont Hydrol 50:99–120

    Article  CAS  Google Scholar 

  • Simonsen A (2010) Physiological characterisation of bacteria degrading dichlobenil and linuron herbicides. PhD thesis, Geological Survey of Denmark & Greenland

  • Simonsen A, Badawi N, Anskjær GG, Albers CN, Sørensen SR, Sørensen J, Aamand J (2012) Intermediate accumulation of metabolites results in a bottleneck for mineralisation of the herbicide metabolite 2,6-dichlorobenzamide (BAM) by Aminobacter spp. Appl Microbiol Biotechnol 94:237–245

    Article  CAS  PubMed  Google Scholar 

  • Sjøholm OR, Aamand J, Sørensen J, Nybroe O (2010a) Degrader density determines spatial variability of 2,6-dichlorobenzamide mineralisation in soil. Environ Poll 158:292–298

    Article  Google Scholar 

  • Sjøholm OR, Nybroe O, Aamand J, Sørensen J (2010b) 2,6-Dichlorobenzamide (BAM) herbicide mineralisation by Aminobacter sp. MSH1 during starvation depends on a subpopulation of intact cells maintaining vital membrane functions. Environ Poll 158:3618–3625

    Article  Google Scholar 

  • Sørensen SR, Aamand J (2003) Rapid mineralisation of the herbicide isoproturon in soil from a previously treated Danish agricultural field. Pest Manag Sci 59:1118–1124

    Article  PubMed  Google Scholar 

  • Sørensen SR, Holtze MS, Simonsen A, Aamand J (2007) Degradation and mineralization of nanomolar concentrations of the herbicide dichlobenil and its persistent metabolite 2,6-dichlorobenzamide by Aminobacter spp. isolated from dichlobenil-treated soils. Appl Environ Microbiol 73:399–406

    Article  PubMed Central  PubMed  Google Scholar 

  • Thorling L, Hansen B, Langtofte L, Brüsch W, Møller RR, Mielby S, Højbjerg AL (2011) Groundwater monitoring 2011 (Grundvandsovervågning 2011) in Danish with English summary (www.grundvandsovervaagning.dk)

  • Tros M, Schraa G, Zehnder AJB, Bosma TNP (1998) Anomalies in the transformation of 3-chlorobenzoate in percolation columns with Pseudomonas sp. strain b13. Wat Sci Technol 37:89–96

    Article  CAS  Google Scholar 

  • Zearley TL, Summers RS (2012) Removal of trace organic micropollutants by drinking water biological filters. Environ Sci Technol 46:9412–9419

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The study was financially supported by the MIRESOWA project (Danish Strategic Research Council grant no. 2104-08-0012). Kerteminde, Hvidovre, Astrup, and Aike Waterworks are thanked for allowing sampling of filter materials.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department Geochemistry, Geological Survey of Denmark & Greenland, Ø. Voldgade 10, 1350, Copenhagen, Denmark

    Christian Nyrop Albers, Ole Stig Jacobsen & Jens Aamand

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  1. Christian Nyrop Albers
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  2. Ole Stig Jacobsen
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  3. Jens Aamand
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Correspondence to Christian Nyrop Albers.

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Albers, C.N., Jacobsen, O.S. & Aamand, J. Using 2,6-dichlorobenzamide (BAM) degrading Aminobacter sp. MSH1 in flow through biofilters—initial adhesion and BAM degradation potentials. Appl Microbiol Biotechnol 98, 957–967 (2014). https://doi.org/10.1007/s00253-013-4942-6

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  • DOI: https://doi.org/10.1007/s00253-013-4942-6

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