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Comparative functional genomics to reveal the molecular basis of phenotypic diversities and guide the genetic breeding of industrial yeast strains

  • Genomics, transcriptomics, proteomics
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

An understanding of the genetic basis underlying the phenotypic variations of yeast strains would guide the breeding of this useful microorganism. Here, comparative functional genomics (CFG) of two bioethanol Saccharomyces cerevisiae strains (YJS329 and ZK2) with different stress tolerances and ethanol fermentation performances were performed. Our analysis indicated that different patterns of gene expression in the central carbon metabolism, antioxidative factors, and membrane compositions of these two strains are the main contributors to their various traits. Some of the differently expressed genes were directly caused by the genomic structural variations between YJS329 and ZK2. Moreover, CFG of these two strains also led to novel insights into the mechanism of stress tolerance in yeast. For example, it was found that more oleic acid in the plasma membrane contributes to the acetic acid tolerance of yeast. Based on the genetic information particular to each strain, strategies to improve their adaptability and ethanol fermentation performances were designed and confirmed. Thus, CFG could not only help reveal basis of phenotypic diversities but also guide the genetic breeding of industrial microorganisms.

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Acknowledgments

This work was financially supported by the State Key Laboratory of Motor Vehicle Biofuels Technology of China, Henan Tianguan Group, Co., Ltd.

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Authors and Affiliations

  1. Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang Province, People’s Republic of China

    Dao-Qiong Zheng, Tian-Zhe Liu, Jie Chen, Ke Zhang, Ou Li, Liang Zhu, Yu-Hua Zhao, Xue-Chang Wu & Pin-Mei Wang

  2. Institute of Marine Biology and Natural Products, College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang Province, People’s Republic of China

    Pin-Mei Wang

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  1. Dao-Qiong Zheng
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  2. Tian-Zhe Liu
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  3. Jie Chen
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  4. Ke Zhang
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  5. Ou Li
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  6. Liang Zhu
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  7. Yu-Hua Zhao
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  8. Xue-Chang Wu
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Correspondence to Xue-Chang Wu or Pin-Mei Wang.

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Zheng, DQ., Liu, TZ., Chen, J. et al. Comparative functional genomics to reveal the molecular basis of phenotypic diversities and guide the genetic breeding of industrial yeast strains. Appl Microbiol Biotechnol 97, 2067–2076 (2013). https://doi.org/10.1007/s00253-013-4698-z

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  • DOI: https://doi.org/10.1007/s00253-013-4698-z

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