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High-coverage gene expression profiling analysis of the cellulase-producing fungus Acremonium cellulolyticus cultured using different carbon sources

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Abstract

The gene expression of a cellulase-producing fungus, Acremonium cellulolyticus, was investigated after culturing with three different carbon sources: glycerol, lactose, and Solka-Floc powdered cellulose (SF). High-coverage gene expression profiling (HiCEP) analysis, a method requiring no prior sequence knowledge, was used to screen genes upregulated at the early stage of cellulase production. SF was used as a strong inducer of cellulase production, lactose was used as an inducer of the expression of cellulase genes at the early stage of the culture, and glycerol was used as a negative control. Approximately 15,000 transcript-derived fragments (TDFs) were detected in each sample prepared from the culture grown for 16 h. Based on the expression profiles of the cultured cells, 36 fragments upregulated in both the SF and lactose cultures were selected and sequenced. The deduced gene products of 31 TDFs were likely related to biomass degradation, sugar metabolism, transcriptional regulation, protein modification and metabolism, cell wall recycling, fatty acid and polyketide biosynthesis, and other functions. Quantitative real-time reverse-transcriptase polymerase chain reaction analysis verified that almost all of the transcripts obtained by HiCEP analysis were upregulated in the SF and lactose cultures grown for 18 h. Some of the TDFs in the SF culture were further upregulated over the course of 72 h. The gene products from these TDFs would provide insight into improving the cellulase productivity of A. cellulolyticus.

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Acknowledgments

This work was supported by the Regional Biomass Energy Project, Ministry of Agriculture, Forestry, and Fisheries, Japan. We thank Dr. Akira Iwase (RIKEN Plant Science Center, Yokohama), Dr. Kinya Sakanishi, Dr. Tomoaki Minowa, Dr. Shinji Fujimoto, Dr. Takashi Yanagida (Biomass Technology Research Center, National Institute of Advanced Industrial Science and Technology), Dr. Seiya Watanabe, and Dr. Yuzuru Tozawa (Ehime University) for their useful comments and valuable discussion.

Conflicts of interest

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Senior Research Fellow Center, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan

    Akihiro Hideno

  2. Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan

    Akihiro Hideno, Hiroyuki Inoue, Tatsuya Fujii, Shinichi Yano, Kenichiro Tsukahara, Katsuji Murakami & Shigeki Sawayama

  3. MessengerScape Co., Ltd., 3-13-11 Chuo, Nakano-ku, Tokyo, 164-0011, Japan

    Harunobu Yunokawa

  4. Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan

    Shigeki Sawayama

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  1. Akihiro Hideno
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  2. Hiroyuki Inoue
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  3. Tatsuya Fujii
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  4. Shinichi Yano
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  5. Kenichiro Tsukahara
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  7. Harunobu Yunokawa
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  8. Shigeki Sawayama
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Correspondence to Hiroyuki Inoue.

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Hideno, A., Inoue, H., Fujii, T. et al. High-coverage gene expression profiling analysis of the cellulase-producing fungus Acremonium cellulolyticus cultured using different carbon sources. Appl Microbiol Biotechnol 97, 5483–5492 (2013). https://doi.org/10.1007/s00253-013-4689-0

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  • DOI: https://doi.org/10.1007/s00253-013-4689-0

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