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Co-digestion of manure and whey for in situ biogas upgrading by the addition of H2: process performance and microbial insights

  • Bioenergy and biofuels
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Abstract

In situ biogas upgrading was conducted by introducing H2 directly to the anaerobic reactor. As H2 addition is associated with consumption of the CO2 in the biogas reactor, pH increased to higher than 8.0 when manure alone was used as substrate. By co-digestion of manure with acidic whey, the pH in the anaerobic reactor with the addition of hydrogen could be maintained below 8.0, which did not have inhibition to the anaerobic process. The H2 distribution systems (diffusers with different pore sizes) and liquid mixing intensities were demonstrated to affect the gas-liquid mass transfer of H2 and the biogas composition. The best biogas composition (75:6.6:18.4) was obtained at stirring speed 150 rpm and using ceramic diffuser, while the biogas in the control reactor consisted of CH4 and CO2 at a ratio of 55:45. The consumed hydrogen was almost completely converted to CH4, and there was no significant accumulation of VFA in the effluent. The study showed that addition of hydrogen had positive effect on the methanogenesis, but had no obvious effect on the acetogenesis. Both hydrogenotrophic methanogenic activity and the concentration of coenzyme F420 involved in methanogenesis were increased. The archaeal community was also altered with the addition of hydrogen, and a Methanothermobacter thermautotrophicus related band appeared in a denaturing gradient gel electrophoresis gel from the sample of the reactor with hydrogen addition. Though the addition of hydrogen increased the dissolved hydrogen concentration, the degradation of propionate was still thermodynamically feasible at the reactor conditions.

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Acknowledgments

This study was funded by the Danish Council for Independent Research (12-126632) and Hans Christian Ørsted Postdoc Program from Technical University of Denmark. The authors would like to thank Hector Garcia for his technical assistance with the experiments.

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Authors and Affiliations

  1. Department of Environmental Engineering, Technical University of Denmark, 2800, Kgs Lyngby, Denmark

    Gang Luo & Irini Angelidaki

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  1. Gang Luo
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  2. Irini Angelidaki
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Correspondence to Irini Angelidaki.

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This file contains methods to estimate carbonate species and mass transfer constant, Fig. S1–S3 and Table S1–S2.

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Luo, G., Angelidaki, I. Co-digestion of manure and whey for in situ biogas upgrading by the addition of H2: process performance and microbial insights. Appl Microbiol Biotechnol 97, 1373–1381 (2013). https://doi.org/10.1007/s00253-012-4547-5

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  • DOI: https://doi.org/10.1007/s00253-012-4547-5

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