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A mechanism for NaCl inhibition of Reactive Blue 19 decolorization and ABTS oxidation by laccase

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Abstract

Laccases produced by white rot fungi have been extensively evaluated for their potential to decolorize textile wastewaters which contain salts like sodium chloride and sodium sulfate. The effect of sodium chloride and sodium sulfate on Trametes versicolor laccase during the decolorization of an anthraquinone dye (Reactive Blue 19) and the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were evaluated by steady-state kinetic analysis. The results showed that, while sodium sulfate did not affect laccase activity, sodium chloride inhibited both ABTS oxidation and dye decolorization. However, the type of inhibition was substrate-dependent: it was hyperbolic, noncompetitive with ABTS and parabolic, noncompetitive with Reactive Blue 19. Furthermore, the results suggested that two chlorides may bind to laccase in the presence of the dye unlike recent inhibition models which suggest that there is only one inhibition site. This investigation is the first to provide evidence for and to propose a two-site model of laccase inhibition, providing new insight into NaCl inhibition of laccase. The proposed model is also useful to predict decolorization rates in the presence of sodium chloride and to determine operating conditions that will minimize inhibition.

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Acknowledgments

This research was financially supported by the Natural Science and Engineering Research Council of Canada; a Premier’s Research Excellence Award, Government of Ontario; and the Chancellor’s Award of Queen’s University as well as a Queen’s Graduate Award to P.-P. Champagne.

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Authors and Affiliations

  1. Chemical Engineering, Queen’s University, Kingston, ON, K7L 3N6, Canada

    P.-P. Champagne & J. A. Ramsay

  2. Biochemistry and Medicine, Queen’s University, Kingston, ON, K7L 3N6, Canada

    M. E. Nesheim

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  1. P.-P. Champagne
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  2. M. E. Nesheim
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  3. J. A. Ramsay
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Correspondence to J. A. Ramsay.

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Champagne, PP., Nesheim, M.E. & Ramsay, J.A. A mechanism for NaCl inhibition of Reactive Blue 19 decolorization and ABTS oxidation by laccase. Appl Microbiol Biotechnol 97, 6263–6269 (2013). https://doi.org/10.1007/s00253-012-4525-y

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