Abstract
Succinic acid is a promising chemical which has a wide range of applications and can be biologically produced. The separation of succinic acid from fermentation broth makes more than 50 % of the total costs in their microbial production. This review summarizes the present state of methods studied for the recovery and purification of biologically produced succinate. Previous studies on the separation of succinic acid primarily include direct crystallization, precipitation, membrane separation, extraction, chromatography, and in situ separation. No single method has proved to be simple and efficient, and improvements are especially needed with regard to yield, purity, and energy consumption. It is argued that separation technologies coupled with upstream technology, in situ product removal, and biorefining strategy deserve more attentions in the future.
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References
Beauprez JJ, De Mery M, Soetaert WK (2010) Microbial succinic acid production: natural versus metabolic engineered producers. Process Biochem 45:1103–1114
Bechthold I, Bretz K, Kabasci S, Kopitzky R, Springer A (2008) Succinic acid: a new platform chemical for bio-based polymers from renewable resources. Chem Eng Technol 5:647–654
Berglund KA, Elankovan P, Glassner DA (1991) Carboxylic acid purification and crystallization process. US Patent 5:034,105
Berglund KA, Yedur S, Dunuwila D (1999) Succinic acid production and purification. US Patent 5:958,744
Cheng KK, Zhao XB, Zeng J, Zhang JA (2012) Biotechnological production of succinic acid—current state and perspectives. Biofuel Bioprod Bior 6:302–318
Cukalovic A, Stevens CV (2008) Feasibility of production methods for succinic acid derivatives: a marriage of renewable resources and chemical technology. Biofuel Bioprod Bior 2:505–529
Datta R (1992) Process for the production of succinic acid by anaerobic fermentation. US Patent 5:143,833
Datta R, Glassner DA, Jain MK, Vick Roy JR (1992) Fermentation and purification process for succinic acid. US Patent 5:168,055
Davison BH, Nghiem NP, Richardson GL (2004) Succinic acid adsorption from fermentation broth and regeneration. Appl Biochem Biotechnol 113:653–669
Delhomme C, Weuster-Botz D, Kühn FE (2009) Succinic acid from renewable resources as a C4 building-block chemical—a review of the catalytic possibilities in aqueous media. Green Chem 11:13–26
Dittrich CR, Bennett GN, San KY (2009) Metabolic Engineering of the anaerobic central metabolic pathway in Escherichia coli for the simultaneous anaerobic production of isoamyl acetate and succinic acid. Biotechnol Prog 25:1304–1309
Efe C, Pieterse M, Van der Wielen LAM, Straathof AJJ (2011) Separation of succinic acid from its salts on a high-silica zeolite bed. Chem Eng Process 50:1143–1151
Glassner DA, Datta R (1992) Process for the production and purification of succinic acid. US Patent 5:143,834
Graaf VDMJ, Valianpoer F, Fiey G, Delattre L, Schulten EAM (2011) Process for the crystallization of succinic acid. WO2011064151
Guettler MV, Jain MK, Rumler D (1996) Method for making succinic acid, bacterial variants for use in the process, and methods for obtaining variants. US Patent 5:573,931
Hepburn AJ, Daugulis AJ (2012) The use of CO2 for reversible pH shifting, and the removal of succinic acid in a polymer-based two-phase partitioning bioreactor. J Chem Technol Biotechnol 87:42–50
Hong YK, Hong WH (2000a) Equilibrium studies on reactive extraction of succinic acid from aqueous solutions with tertiary amines. Bioprocess Eng 22:477–481
Hong YK, Hong WH (2000b) Reactive extraction of succinic acid with tripropylamine (TPA) in various diluents. Bioprocess Eng 22:281–284
Huh YS, Jun YS, Hong YK, Song H, Lee SY, Hong WH (2006) Effective purification of succinic acid from fermentation broth produced by Mannheimia succiniciproducens. Process Biochem 41:1461–1465
Inci I, Bayazit SS, Asci YS (2011) Separation of succinic acid from aqueous solution by alumina adsorption. J Chem Eng Data 56:4449–4453
Inci I, Asci YS, Uslu H (2012) LSER modeling of extraction of succinic acid by tridodecylamine dissolved in 2-octanone and 1-octanol. J Ind Eng Chem 18:152–159
Jantama K, Haupt MJ, Svoronos SA, Zhang X, Moore JC, Shanmugam KT, Ingram LO (2008) Combining metabolic engineering and metabolic evolution to develop non-recombinant strains of Escherichia coli C that produce succinate and malate. Biotechnol Bioeng 99:1140–1153
Jun YS, Huh YS, Park HS, Thomas A, Jeon SJ, Lee EZ, Won HJ, Hong WH, Lee SY, Hong YK (2007a) Adsorption of pyruvic and succinic acid by amine-functionalized SBA-15 for the purification of succinic acid from fermentation broth. J Phys Chem C 111:13076–13086
Jun YS, Lee EZ, Huh YS, Hong YK, Hong WH, Lee SY (2007b) Kinetic study for the extraction of succinic acid with TOA in fermentation broth; effects of pH, salt and contaminated acid. Biochem Eng J 36:8–13
Kang Z, Gao CJ, Wang Q, Liu HM, Qi QS (2010) A novel strategy for succinate and polyhydroxybutyrate co-production in Escherichia coli. Bioresour Technol 101:7675–7678
Kertes AS, King CJ (1985) Extraction chemistry of fermentation product carboxylic acids. Biotechnol Bioeng 28:269
Kurzrock T, Weuster-Botz D (2010) Recovery of succinic acid from fermentation broth. Biotech Lett 32:331–339
Kurzrock T, Weuster-Botz D (2011) New reactive extraction systems for separation of bio-succinic acid. Bioprocess Biosys Eng 34:779–787
Lee SC (2011) Extraction of succinic acid from simulated media by emulsion liquid membranes. J Membr Sci 381:237–243
Lee PC, Lee WG, Lee SY, Chang HN (2001) Succinic acid production with reduced by-product formation in the fermentation of Anaerobiospirillum succiniciproducens using glycerol as a carbon source. Biotechnol Bioeng 72:41–48
Lee SJ, Song H, Lee SY (2006) Genome-based metabolic engineering of Mannheimia succiniciproducens for succinic acid production. Appl Environ Microbiol 72:1939–1948
Li Q, Li WL, Wang D, Liu BB, Tang H, Yang MH, Liu QF, Xing JM, Su ZG (2010a) pH neutralization while succinic acid adsorption onto anion-exchange resins. Appl Biochem Biotechnol 160:438–445
Li Q, Wang D, Wu Y, Li WL, Zhang YJ, Xing JM, Su ZG (2010b) One step recovery of succinic acid from fermentation broths by crystallization. Sep Purif Technol 72:294–300
Li Q, Wang D, Hu GY, Xing JM, Su ZG (2011) Integrated bioprocess for high-efficiency production of succinic acid in an expanded-bed adsorption system. Biochem Eng J 56:150–157
Lin SKC, Du CY, Blaga AC, Camarut M, Webb C, Stevens CV, Soetaert W (2010) Novel resin-based vacuum distillation–crystallisation method for recovery of succinic acid crystals from fermentation broths. Green Chem 12:666–671
Lu SF, Chen M, Chen CH (2012) Mechanisms and kinetics of thermal degradation of poly(butylene succinate-co-propylene succinate)s. J Appl Polym Sci 123:3610–3619
Luque R, Lin CSK, Du C, Macquarrie DJ, Koutinas A, Wang R, Webb C, Clark JH (2009) Chemical transformations of succinic acid recovered from fermentation broths by a novel direct vacuum distillation–crystallisation method. Green Chem 11:193–200
Nam HG, Park KM, Lim SS, Mun S (2011) Adsorption equilibria of succinic acid and lactic acid on Amberchrom CG300C resin. J Chem Eng Data 56:464–471
Okino S, Noburyu R, Suda M, Jojima T, Inui M, Yukawa H (2008) An efficient succinic acid production process in a metabolically engineered Corynebacterium glutamicum strain. Appl Microbiol Biot 81:459–464
Orjuela A, Yanez AJ, Peereboom L, Lira CT, Miller DJ (2011) A novel process for recovery of fermentation-derived succinic acid. Sep Purif Technol 83:31–37
Ponnampalam E (1999) Purification of organic acids using anion exchange chromatography. WO Patent 9:944,707
Song H, Huh YS, Lee SY, Hong WH, Hong YK (2007) Recovery of succinic acid produced by fermentation of a metabolically engineered Mannheimia succiniciproducens strain. J Biotechnol 132:445–452
Straathof AJJ, Efe C, Engel CAR, Garzon CSL, Van der Wielen LAM (2010) Fermentation and recovery of bio-based fumaric and succinic acid. J Biotechnol 150:82–83
U.S. Department of Energy (2004) Top value added chemicals from biomass; volume 1: results of screening for potential candidates from sugars and synthesis gas
Vemuri GN, Eiteman MA, Altman E (2002) Succinate production in dual-phase Escherichia coli fermentations depends on the time of transition from aerobic to anaerobic conditions. J Ind Microbiol Biot 28:325–332
Wang D, Li QA, Yang MH, Zhang YJ, Su ZG, Xing JM (2011) Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation. Process Biochem 46:365–371
Wu H, Jiang M, Wei P, Lei D, Yao Z, Zuo P (2011a) Nanofiltration method for separation of succinic acid from its fermented broth. Chinese Patent CN200910025531.5
Wu RC, Xu YZ, Song YQ, Luo JA, Liu DH (2011b) A novel strategy for salts recovery from 1,3-propanediol fermentation broth by bipolar membrane electrodialysis. Sep Purif Technol 83:9–14
Xu J, Guo BH (2010) Poly (butylene succinate) and its copolymers: research, development and industrialization. Biotechnol J 11:1149–1163
Xu YZ, Guo NN, Zheng ZM, Ou XJ, Liu HJ, Liu DH (2009) Metabolism in 1, 3-propanediol fed-batch fermentation by a D-lactate deficient mutant of Klebsiella pneumoniae. Biotechnol Bioeng 104:965–972
Yao Z, Wu H, Liu H, Li S, Jiang M (2008) Method for separation succinic acid from anaerobic fermentation broth. Chinese Patent CN200610086003.7
Yedur S, Berglung KS, Dunuwila DD (2001) Succinic acid production and purification. US Patent 6:265,190
Zeikus GJ, Jain MK, Elankovan P (1999) Biotechnology of succinic acid production and markets for derived industrial products. Appl Microbiol Biotechnol 51:545–552
Zheng LC, Li CC, Guan GH, Zhang D, Xiao YN, Wang DJ (2011) Investigation on isothermal crystallization, melting behaviors, and spherulitic morphologies of multiblock copolymers containing poly(butylene succinate) and poly(1,2-propylene succinate). J Appl Polym Sci 119:2124–2134
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This study was supported by National Natural Science Foundation of China (21176139), National Basic Research Program of China (2011CB707406), Shanxi Pillar Program (20090322025).
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Cheng, KK., Zhao, XB., Zeng, J. et al. Downstream processing of biotechnological produced succinic acid. Appl Microbiol Biotechnol 95, 841–850 (2012). https://doi.org/10.1007/s00253-012-4214-x
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DOI: https://doi.org/10.1007/s00253-012-4214-x