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Capability of yeast derivatives to adhere enteropathogenic bacteria and to modulate cells of the innate immune system

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Abstract

Yeast derivatives including yeast cell wall components are promising alternatives to antibiotics with respect to the promotion of health and performance in livestock, based on their capacity to bind enteropathogenic bacteria and to beneficially modulate the immune system. However, these mode(s) of action both in vitro and in vivo are still not well understood. Furthermore, standardization and reproducibility of in vitro techniques (microbiology, cell culture assays) are critical features for the application of yeast derivatives as well as for the proof of effectiveness. Yeast cell wall products are suggested as anti-adhesive agents and are thus proposed to prevent attachment of certain intestinal bacteria by providing alternative adhesion sites to enterobacteria, which contain mannose-specific type I fimbriae such as Escherichia coli or Salmonella spp. and which is well documented. Various in vitro assay techniques have become of paramount importance for biotechnological research since they allow for determination and quantification of potential mode(s) of action. However, in vitro assays may be criticized by product end users as not accurately reflecting in vivo responses. Pro and cons of different assays and their bias will be discussed specifically regarding yeast cell wall components and adhesion of enteropathogenic bacteria. Immunomodulation is a therapeutic approach intervening in auto-regulating processes of the defense system. Yeast derivatives such as beta-glucans are proposed to interact with cells of the innate immune system by receptor recognition. Controversial data in literature and mode(s) of action are reviewed and discussed here.

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References

  • Applegate TJ (2009) Influence of phytogenics on the immunity of livestock and poultry. In: Steiner T (ed) Phytogenics in animal nutrition. Nottingham, Nottingham, pp 39–59

    Google Scholar 

  • Applegate TJ, Klose V, Steiner T, Ganner A, Schatzmayr G (2010) Probiotics and phytogenics for poultry: myth or reality? J Appl Poultry Res 19:194–210

    Article  CAS  Google Scholar 

  • Baurhoo B, Phillip L, Ruiz-Feria CA (2007) Effects of purified lignin and mannan oligosaccharides on intestinal integrity and microbial populations in the ceca and litter of broiler chickens. Poultry Sci 86:1070–1078

    Article  CAS  Google Scholar 

  • Baurhoo B, Goldflus F, Zhao X (2009) Purified cell wall of Saccharomyces cerevisiae increases protection against intestinal pathogens in broiler chickens. Int J Poultry Sci 8:133–137

    Article  Google Scholar 

  • Becker PM (2005) Physiological Achilles’ heels of enteropathogenic bacteria in livestock. Curr Iss Intest Microbiol 6:31–54

    Google Scholar 

  • Becker PM, Galletti S (2008) Food and feed components for gut health-promoting adhesion of E. coli and Salmonella enterica. J Sci Food Agr 88:2026–2035

    Article  CAS  Google Scholar 

  • Becker PM, Galletti S, Roubos-van den Hil PJ, van Wikselaar PG (2007) Validation of growth as measurand for bacterial adhesion to food and feed ingredients. J Appl Microbiol 103:2686–2696

    Article  CAS  Google Scholar 

  • Brown GD, Gordon S (2001) A new receptor for beta-glucans. Nature 413:36–37

    Article  CAS  Google Scholar 

  • Dinarello CA (1999) Interleukin-18. Methods: A Companion to Method Enzymol 19:121–132

    Article  CAS  Google Scholar 

  • Dinarello CA (2000) Interleukin-18, a proinflammatory cytokine. Eur Cytokine Netw 11:483–486

    CAS  Google Scholar 

  • Elmadfa I, Leitzmann C (2000) Ernährung des Menschen. Eugen Ulmer, Stuttgart

    Google Scholar 

  • Engstad RE, Robertsen B (1994) Specificity of a beta-glucan receptor on macrophages from Atlantic salmon (Salmo salar L.). Dev Comp Immunol 18:397–408

    Article  CAS  Google Scholar 

  • Ferket PR, Parks CW, Grimes JL (2002) Benefits of dietary antibiotic and mannanoligosaccharides supplementation for poultry. 22 Pages. In: Proc. Multi-State Poult. Feeding and Nutr. Conf., Indianapolis. 14–16, 2002

  • Firon N, Ofek I, Sharon N (1984) Carbohydrate-binding sites of the mannose-specific fimbrial lectins of enterobacteria. Infect Immun 43:1088–1090

    CAS  Google Scholar 

  • Forstner JF, Forstner GG (1994) Gastrointestinal mucus. In: Johnson LR (ed) Physiology of the gastrointestinal tract. Raven, New York, pp 1255–1283

    Google Scholar 

  • Fuller R (1989) Probiotics in man and animals. J Appl Bacteriol 66:365–378

    Article  CAS  Google Scholar 

  • Gallova L, Kubala L, Ciz M, Lojek A (2004) IL-10 does not affect oxidative burst and expression of selected surface antigen on human blood phagocytes in vitro. Physiol Res 53:199–208

    CAS  Google Scholar 

  • Ganner A, Fink L, Schatzmayr G (2008) Quantitative in vitro assay to evaluate yeast products concerning their binding activity of enteropathogenic bacteria. J Anim Sci 86(E.suppl 2):54

    Google Scholar 

  • Ganner A, Nitsch S, Erlacher K, Klimitsch A, Schatzmayer G (2010a) Ex vivo effect of yeast beta-glucan on lymphocyte viability and plasma IL-18 in weaning piglets. Livest Sci 133(1–3):246–248

    Article  Google Scholar 

  • Ganner A, Stoiber C, Wieder D, Schatzmayr G (2010b) Quantitative in vitro assay to evaluate the capability of yeast cell wall fractions from Trichosporon mycotoxinivorans to selectively bind gram negative pathogens. J Microbiol Meth 83:168–174

    Article  CAS  Google Scholar 

  • Gibson GR, Wang X (1994) Regulatory effects of bifidobacteria on the growth of other colonic bacteria. J Appl Bacteriol 77:412–420

    Article  CAS  Google Scholar 

  • Harmon BG (1998) Avian heterophils in inflammation and disease resistance. Poultry Sci 77:972–977

    CAS  Google Scholar 

  • Huff GR, Fernell MB, Huff WE, Rath N, Solis de Santos F, Donoghue AM (2007) Effects of a dietary yeast extract on hematological parameters, heterophil function, and bacterial clearance in turkey poults challenged with Escherichia coli and subjected to transport stress. 16th European Symposium on Poultry Nutrition

  • Kataoka K, Muta T, Yamazaki S, Takeshige K (2002) Activation of macrophages by linear (1,3)-beta-d-glucans. Implications for the recognition of fungi by innate immunity. J Biol Chem 277:36825–36831

    Article  CAS  Google Scholar 

  • Klasing KC (2010) Is immunomodulation good? J Anim Sci 88(E-suppl2):559

    Google Scholar 

  • Klis FM, Boorsma A, De Grot PWJ (2006) Cell wall construction in Saccharomyces cerevisiae. Yeast 23:185–202

    Article  CAS  Google Scholar 

  • Kogan G, Kocher A (2007) Role of yeast cell wall polysaccharides in pig nutrition and health protection. Livest Sci 109:161–165

    Article  Google Scholar 

  • Kogut MH (2000) Cytokines and prevention of infectious diseases in poultry: a review. Avian Pathol 29:395–404

    Article  CAS  Google Scholar 

  • Kulicke WM, Lettau AI, Thielking H (1997) Correlation between immunological activity, molar mass, and molecular structure of different 1,3-beta-d-glucans. Carbohyd Res 297:135–143

    Article  CAS  Google Scholar 

  • Li J, Xing J, Li D, Xu W, Zhao L, Lv S, Huang D (2005) Effects of beta-glucan extracted from Saccharomyces cerevisiae on humoral and cellular immunity in weaned piglets. Arch Anim Nutr 59:303–312

    Article  CAS  Google Scholar 

  • Li J, Li DF, Xing JJ, Cheng ZB, Lai CH (2006) Effects of beta-glucan extracted from Saccharomyces cerevisiae on growth performance, and immunological and somatotropic responses of pigs challenged with Escherichia coli lipopolysaccharide. J Anim Sci 84:2374–2381

    Article  CAS  Google Scholar 

  • Mao XF, Piao XS, Lai CH, Li DF, Xing JJ, Shi BL (2005) Effects of beta-glucan obtained from the Chinese herb Astragalus membranaceus and lipopolysaccharide challenge on performance, immunological, adrenal, and somatotropic responses of weanling pigs. J Anim Sci 83:2775–2782

    CAS  Google Scholar 

  • Marteau PR, De Vrese M, Cellier CJ, Schrezenmeir J (2001) Protection from gastrointestinal diseases with the use of probiotics. Am J Clin Nutr 73

  • Miettinen M, Matikainen S, Vuopio-Varkila J, Pirhonen J, Varkila K, Kurimoto M, Julkunen I (1998) Lactobacilli and streptococci induce interleukin-12 (IL-12), IL-18, and gamma interferon production in human peripheral blood mononuclear cells. Infect Immun 66:6058–6062

    CAS  Google Scholar 

  • Mirelman D, Altmann G, Eshdat Y (1980) Screening of bacterial isolates for mannose-specific lectin activity by agglutination of yeasts. J Clin Microbiol 11:328–331

    CAS  Google Scholar 

  • Molnar O, Schatzmayr G, Fuchs E, Prillinger H (2004) Trichosporon mycotoxinivorans sp. nov., a new yeast species useful in biological detoxification of various mycotoxins. Syst Appl Microbiol 27:661–671

    Article  CAS  Google Scholar 

  • Mourao JL, Pinheiro V, Alves A, Guedes CM, Pinto L, Saavedra MJ, Spring P, Kocher A (2006) Effect of mannan oligosaccharides on the performance, intestinal morphology and cecal fermentation of fattening rabbits. Anim Feed Sci Tech 126:107–120

    Article  CAS  Google Scholar 

  • Much P, Pichler J, Allerberger F (2007) Food borne infectious outbreaks, Austria 2005. Wien Klin Wochenschr 119:150–157

    Article  Google Scholar 

  • Newman K (2006) Quantifying the efficacy of MOS. Feed Mix 14:2–4

    Google Scholar 

  • Ofek I, Beachey EH (1978) Mannose binding and epithelial cell adherence of Escherichia coli. Infect Immun 22:247–254

    CAS  Google Scholar 

  • Ofek I, Mirelman D, Sharon N (1977) Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors. Nature 265:623–625

    Article  CAS  Google Scholar 

  • Ofek I, Hasty DL, Sharon N (2003) Anti-adhesion therapy of bacterial diseases: prospects and problems. FEMS Immunol Med Mic 38:181–191

    Article  CAS  Google Scholar 

  • Okazaki M, Adachi Y, Ohno N, Yadomae T (1995) Structure-activity relationship of beta-d-glucans in the induction of cytokine production from macrophages, in vitro. Biol Pharm Bull 18:1320–1327

    Article  CAS  Google Scholar 

  • Oyofo BA, Droleskey RE, Norman JO, Mollenhauer HH, Ziprin RL, Corrier DE, De Loach JR (1989) Inhibition by mannose of in vitro colonization of chicken small intestine by Salmonella typhimurium. Poultry Sci 68:1351–1356

    Article  CAS  Google Scholar 

  • Pelczar JM, Chan ECS, Krieg NR (1986) Microbiology, 5th edn. McGraw-Hill, Inc, New York, pp 687–700

    Google Scholar 

  • Pie S, Lalles JP, Blazy F, Laffitte J, Seve B, Oswald IP (2004) Weaning is associated with an upregulation of expression of inflammatory cytokines in the intestine of piglets. J Nutr 134:641–647

    CAS  Google Scholar 

  • Raa J (1996) The use of immunostimulatory substances in fish and shellfish farming. Rev Fish Sci 4:229–288

    Article  Google Scholar 

  • Reisinger N, Ganner A, Masching S, Schatzmayer G, Applegate TJ (2011) Efficacy of a yeast derivative on broiler performance, intestinal morphology and blood profile. Livest Sci 143(2–3):195–200

    Google Scholar 

  • Saarela M, Crittenden R, Salminen S, Mattila-Sandholm T (2002) Gut bacteria and health foods—the European perspective. Int J Food Microbiol 78:99–117

    Article  CAS  Google Scholar 

  • Sharon N, Ofek I (2000) Safe as mother’s milk: carbohydrates as future anti-adhesion drugs for bacterial diseases. Glycoconj J 17:659–664

    Article  CAS  Google Scholar 

  • Shoaf-Sweeney KD, Hutkins RW (2008) Chapter 2 adherence, anti-adherence, and oligosaccharides. Preventing Pathogens from Sticking to the Host, 101–161

  • Smith JA (1994) Neutrophils, host defense, and inflammation: a double-edged sword. J Leukocyte Biol 56:672–686

    CAS  Google Scholar 

  • Snellings NJ, Tall BD, Venkatesan MM (1997) Characterization of Shigella type 1 fimbriae: expression, FimA sequence, and phase variation. Infect Immun 65:2462–2467

    CAS  Google Scholar 

  • Sokurenko EV, Courtney HS, Maslow J, Siitonen A, Hasty DL (1995) Quantitative differences in adhesiveness of type 1 fimbriated Escherichia coli due to structural differences in fimH genes. J Bacteriol 177:3680–3686

    CAS  Google Scholar 

  • Solis De Los Santos F, Donoghue AM, Farnel MB, Huff GR, Huff WE, Donoghue DJ (2007) Gastrointestinal maturation is accelerated in turkey poults supplemented with a mannan-oligosaccharide yeast extract (Alphamune). Poultry Sci 86:921–930

    CAS  Google Scholar 

  • Sonnenburg JL, Angenent LT, Gordon JI (2004) Getting a grip on things: how do communities of bacterial symbionts become established in our intestine? Nat Immunol 5:569–573

    Article  CAS  Google Scholar 

  • Spring P, Wenk C, Dawson KA, Newman KE (2000) The Effects of Dietary Mannanoligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of Salmonella-challenged broiler chicks. Poultry Sci 79:205–211

    CAS  Google Scholar 

  • Stuyven E, Cox E, Vancaeneghem S, Arnouts S, Deprez P, Goddeeris BM (2009) Effect of beta-glucans on an ETEC infection in piglets. Vet Immunol Immunop 128:60–66

    Article  CAS  Google Scholar 

  • Terre M, Calvo MA, Adelantado C, Kocher A, Bach A (2007) Effects of mannan oligosaccharides on performance and microorganism fecal counts of calves following an enhanced-growth feeding program. Anim Feed Sci Tech 137:115–125

    Article  CAS  Google Scholar 

  • Tsukada C, Yokoyama H, Miyaji C, Ishimoto Y, Kawamura H, Abo T (2003) Immunopotentiation of intraepithelial lymphocytes in the intestine by oral administrations of beta-glucan. Cell Immunol 221:1–5

    Article  CAS  Google Scholar 

  • Vimal DB, Khullar M, Gupta S, Ganguly NK (2000) Intestinal mucins: the binding sites for Salmonella typhimurium. Mol Cell Biochem 204:107–117

    Article  CAS  Google Scholar 

  • Volman JJ, Ramakers JD, Plat J (2008) Dietary modulation of immune function by beta-d-glucans. Physiol Behav 94:276–284

    Article  CAS  Google Scholar 

  • Walker GM (2000) Yeast physiology and biotechnology. Wiley, Chichester

    Google Scholar 

  • Xia X, Vetvicka V, Yan J, Hanikkyrova M, Mayadas T, Ross GD (1999) The beta-glucan-binding lectin site of mouse CR3 (CD11b/CD18) and its function in generating a primed state of the receptor that mediates cytotoxic activation in response to iC3b-opsonized target cells. J Immunol 162:2281–2290

    CAS  Google Scholar 

  • Yadomae T (2000) Structure and biological activities of fungal beta-glucans. Yakugaku Zasshi 120:413–431

    CAS  Google Scholar 

  • Yang Y, Iji PA, Kocher A, Mikkelsen LL, Choct M (2008) Effects of mannanoligosaccharide and fructooligosaccharide on the response of broilers to pathogenic Escherichia coli challenge. Brit Poultry Sci 49:550–559

    Article  CAS  Google Scholar 

  • Zopf D, Roth S (1996) Oligosaccharide anti-infective agents. Lancet 347:1017–1021

    Article  CAS  Google Scholar 

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Acknowledgments

I would like to thank Dietmar Haltrich for critically reading the manuscript and for his excellent and kind support.

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  1. BIOMIN Research Center, Technopark 1, 3430, Tulln, Austria

    Anja Ganner & Gerd Schatzmayr

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  1. Anja Ganner
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  2. Gerd Schatzmayr
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Correspondence to Anja Ganner.

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Ganner, A., Schatzmayr, G. Capability of yeast derivatives to adhere enteropathogenic bacteria and to modulate cells of the innate immune system. Appl Microbiol Biotechnol 95, 289–297 (2012). https://doi.org/10.1007/s00253-012-4140-y

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