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Ethanol production from concentrated food waste hydrolysates with yeast cells immobilized on corn stalk

  • Bioenergy and biofuels
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Abstract

The aim of the present study was to examine ethanol production from concentrated food waste hydrolysates using whole cells of S. cerevisiae immobilized on corn stalks. In order to improve cell immobilization efficiency, biological modification of the carrier was carried out by cellulase hydrolysis. The results show that proper modification of the carrier with cellulase hydrolysis was suitable for cell immobilization. The mechanism proposed, cellulase hydrolysis, not only increased the immobilized cell concentration, but also disrupted the sleek surface to become rough and porous, which enhanced ethanol production. In batch fermentation with an initial reducing sugar concentration of 202.64 ± 1.86 g/l, an optimal ethanol concentration of 87.91 ± 1.98 g/l was obtained using a modified corn stalk-immobilized cell system. The ethanol concentration produced by the immobilized cells was 6.9% higher than that produced by the free cells. Ethanol production in the 14th cycle repeated batch fermentation demonstrated the enhanced stability of the immobilized yeast cells. Under continuous fermentation in an immobilized cell reactor, the maximum ethanol concentration of 84.85 g/l, and the highest ethanol yield of 0.43 g/g (of reducing sugar) were achieved at hydraulic retention time (HRT) of 3.10 h, whereas the maximum volumetric ethanol productivity of 43.54 g/l/h was observed at a HRT of 1.55 h.

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Acknowledgments

The present research was financially supported by the Chinese National Science and Technology Pillar Program in the Eleventh Five-year Plan Period (Grant No. 2007BAD89B10-5(5)).

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Authors and Affiliations

  1. School of Life Science, Huainan Normal University, Huainan, Anhui, 232001, People’s Republic of China

    Shoubao Yan

  2. Key Laboratory of Ion Beam Bio-engineering of Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031, People’s Republic of China

    Xiangsong Chen, Jingyong Wu & Pingchao Wang

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  1. Shoubao Yan
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  2. Xiangsong Chen
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  3. Jingyong Wu
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  4. Pingchao Wang
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Correspondence to Shoubao Yan.

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Yan, S., Chen, X., Wu, J. et al. Ethanol production from concentrated food waste hydrolysates with yeast cells immobilized on corn stalk. Appl Microbiol Biotechnol 94, 829–838 (2012). https://doi.org/10.1007/s00253-012-3990-7

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  • DOI: https://doi.org/10.1007/s00253-012-3990-7

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