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Characterization of the mitochondrial NAD+-dependent isocitrate dehydrogenase of the oleaginous yeast Rhodosporidium toruloides

  • Applied microbial and cell physiology
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Abstract

Early biochemical studies have demonstrated that lipid accumulation by oleaginous yeasts is linked to the activity of the NAD+-dependent isocitrate dehydrogenase (Idh). However, molecular study of Idh of oleaginous microorganisms remains limited. Here, we present the cloning of a mitochondrial NAD+-specific Idh from Rhodosporidium toruloides (RtIdh), an excellent microbial lipid producer that uses carbohydrates as the carbon source. The evolutionary relationship analyses among RtIdhs and other yeast Idhs revealed that RtIdh had a closer relationship with the Idhs of Ustilago maydis and Schizophyllum commune. We expressed the RtIDH gene in the yeast Saccharomyces cerevisiae idhΔ mutant. Under the nitrogen-limited condition, the intracellular lipid content and extracellular citrate concentration of the culture of the S. cerevisiae idhΔ carrying the RtIDH gene increased as the carbon/nitrogen molar ratio of the media increased, while the wild-type S. cerevisiae strain showed no correlation. Our data provided valuable information for elucidating the molecular mechanism of microbial oleaginicity and for engineering microorganisms to produce metabolites of fatty acid pathway.

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Acknowledgments

Financial supports provided by the Natural Sciences Foundation of China (31000052) and the Knowledge Innovation Program of CAS (KSCX2-EW-G-1-3) are greatly acknowledged.

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Authors and Affiliations

  1. Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China

    Fan Yang, Sufang Zhang, Yongjin J. Zhou, Zhiwei Zhu, Xinping Lin & Zongbao K. Zhao

  2. School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China

    Fan Yang

  3. Dalian National Laboratory for Clean Energy, Dalian, 116023, China

    Zongbao K. Zhao

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  1. Fan Yang
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  2. Sufang Zhang
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  3. Yongjin J. Zhou
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  4. Zhiwei Zhu
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  5. Xinping Lin
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  6. Zongbao K. Zhao
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Correspondence to Zongbao K. Zhao.

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Yang, F., Zhang, S., Zhou, Y.J. et al. Characterization of the mitochondrial NAD+-dependent isocitrate dehydrogenase of the oleaginous yeast Rhodosporidium toruloides . Appl Microbiol Biotechnol 94, 1095–1105 (2012). https://doi.org/10.1007/s00253-011-3820-3

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  • DOI: https://doi.org/10.1007/s00253-011-3820-3

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