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Characterization of a new ScbR-like γ-butyrolactone binding regulator (SlbR) in Streptomyces coelicolor

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Abstract

γ-Butyrolactones in Streptomyces are well recognized as bacterial hormones, and they affect secondary metabolism of Streptomyces. γ-Butyrolactone receptors are considered important regulatory proteins, and various γ-butyrolactone synthases and receptors have been reported in Streptomyces. Here, we characterized a new regulator, SCO0608, that interacted with SCB1 (γ-butyrolactone of Streptomyces coelicolor) and bound to the scbR/A and adpA promoters. The SCO0608 protein sequences are not similar to those of any known γ-butyrolactone binding proteins in Streptomyces such as ScbR from S. coelicolor or ArpA from Streptomyces griseus. Interestingly, SCO0608 functions as a repressor of antibiotic biosynthesis and spore formation in R5 complex media. We showed the existence of another type of γ-butyrolactone receptor in Streptomyces, and this SCO0608 was named ScbR-like γ-butyrolactone binding regulator (SlbR) in S. coelicolor.

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Acknowledgments

The authors thank John Innes Center for providing the cosmid library. This work was supported by the Global Frontier Program for Intelligent Synthetic Biology, Basic Science Research Program (2010-0009942) and Priority Research Centers Program (2009-0094021) through the National Research Foundation grant funded by the Korea Government (MEST). This subject is also partially supported by the Korea Ministry of Environment as a “Converging Technology Project (201-101-007).”

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Authors and Affiliations

  1. Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, South Korea

    Yung-Hun Yang, Jong-Min Jeon & Thangamani Rajesh

  2. School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, and Institute of Bioengineering, Seoul National University, Seoul, South Korea

    Eunjung Song, Ji-Nu Kim, Bo-Rahm Lee, Eun-Jung Kim, Sung-Hee Park, Woo-Seong Kim, Yun-Gon Kim & Byung-Gee Kim

  3. Bio-MAX Institute, Seoul National University, Seoul, South Korea

    Hyung-Yeon Park

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  1. Yung-Hun Yang
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Yang, YH., Song, E., Kim, JN. et al. Characterization of a new ScbR-like γ-butyrolactone binding regulator (SlbR) in Streptomyces coelicolor . Appl Microbiol Biotechnol 96, 113–121 (2012). https://doi.org/10.1007/s00253-011-3803-4

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