Abstract
Internal airlift reactors are closed systems considered today for microalgae cultivation. Several works have studied their hydrodynamics but based on important solid concentrations, not with biomass concentrations usually found in microalgae cultures. In this study, an internal airlift reactor has been built and tested in order to clarify the hydrodynamics of this system, based on microalgae typical concentrations. A model is proposed taking into account the variation of air bubble velocity according to volumetric air flow rate injected into the system. A relationship between riser and downcomer gas holdups is established, which varied slightly with solids concentrations. The repartition of solids along the reactor resulted to be homogenous for the range of concentrations and volumetric air flow rate studied here. Liquid velocities increase with volumetric air flow rate, and they vary slightly when solids are added to the system. Finally, liquid circulation time found in each section of the reactor is in concordance with those employed in microalgae culture.
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References
Bakker W, van Can H, Tramper J, de Gooijer C (1993) Hydrodynamics and mixing in a multiple air-lift loop reactor. Biotechnol Bioeng 42:994–1001
Bello R (1981) A characterization study of airlift contactors for application to fermentations. Ph.D. thesis, University of Waterloo, Ontario, Canada
Blazej M, Kisa M, Markos J (2004) Scale influence on the hydrodynamics of an internal airlift loop airlift reactor. Chem Eng Process 43:1519–1527
Brindley C, García M, Acién F, Fernández J, García J, Molina E (2004) Influence of power supply in the feasibility of Phaeodactylum tricornutum cultures. Biotechnol Bioeng 87:723–733
Chisti M (1989) Airlift bioreactors. Elsevier Science, London
Chisti Y, Wenge F, Moo-Young M (1995) Relationship between riser and downcomer gas holdup in internal airlift reactors without gas–liquid separators. Chem Eng J 57:B7–B13
Contreras A, García F, Molina E, Merchuk J (1998a) Interaction between CO2-mass transfer, light availability, and hydrodynamic stress in the growth of Phaeodactylum tricornutum in a concentric tube airlift photobioreactor. Biotechnol Bioeng 60:317–325
Contreras A, Chisti Y, Molina E (1998b) A reassessment of relationship between riser and downcomer gas holdups in airlift reactors. Chem Eng Sci 53:4151–4158
Contreras A, Garcia F, Molina E, Merchuk J (1999) Influence of sparger on energy dissipation, shear rate, and mass transfer to sea water in a concentric-tube airlift bioreactor. Enzyme Microb Technol 25:820–830
Converti A, Lodi A, Del Borghi A, Solisio C (2006) Cultivation of Spirulina platensis in a combined airlift-tubular reactor system. Biochem Eng J 32:13–18
Fadavi A, Chisti Y (2007) Gas holdup and mixing characteristics of a novel forced circulation loop reactor. Chem Eng J 131:105–111
Freitas C, Teixeira J (1998a) Hydrodynamic studies in an airlift reactor with an enlarged degassing zone. Bioprocess Eng 18:267–279
Freitas C, Teixeira J (1998b) Solid-phase distribution in an airlift reactor with an enlarged degassing zone. Biotechnol Tech 12:219–224
Freitas C, Fialova M, Zahradnik J, Teixeira J (1999) Hydrodynamic model for three-phase internal- and external-loop airlift reactors. Chem Eng Sci 54:5253–5258
Ganzeveld K, Chisti Y, Moo-Young M (1995) Hydrodynamic behaviour of animal cell microcarrier suspensions in split-cylinder airlift bioreactors. Bioprocess Eng 12:239–247
García J, Lavin A, Diaz M (2000) High liquid holdup airlift tower loop reactor: I. Riser hydrodynamic characteristics. J Chem Technol 75:369–377
Giaveno A, Lavalle L, Chiacchiarini P, Donati E (2007) Microbial processing of metal sulfides. Springer, Dordrecht
Heijnen J, Hols J, Van der Lans R, Van Leeuwen H, Mulder A, Weltevrede R (1997) A simple hydrodynamic model for the liquid circulation velocity in a full-scale two- and three-phase internal airlift reactor operating in the gas recirculation regime. Chem Eng Sci 52:2527–2540
Janssen M, Kuijpers T, Veldhoen B, Ternbach M, Tramper J, Mur L, Weijffels R (1999) Specific growth rate of Chlamydomonas reinhardtii and Chlorella sorokiniana under medium duration light:dark cycles: 13–87 s. J Biotechnol 70:323–333
Janssen M., Tramper J., Mur L., Wijffels R. (2003) Enclosed Outdoor Photobioreactors: Light Regime, Photosynthetic Efficiency, Scale-Up, and Future Prospects. Biotechnol Bioeng, 81:193–210
Jin B, Lant P (2004) Flow regime, hydrodynamics, floc size distribution and sludge properties in activated sludge bubble column, air-lift and aerated stirred reactors. Chem Eng Sci 59:2379–2388
Levy S (1999) Two phase flow in complex systems. Wiley-Interscience, USA
Livingston A, Zhang S (1993) Hydrodynamic behaviour of three-phase (gas–liquid–solid) airlift reactors. Chem Eng Sci 48:1641–1654
Lu W, Hwang S, Chang C (1994) Liquid velocity and gas holdup in three-phase internal loop airlift reactors with low-density particles. Chem Eng Sci 50:1301–1310
Luo H, Muthanna H (2008) Local characteristics of hydrodynamics in draft tube airlift bioreactor. Chem Eng Sci 63:3057–3068
Merchuk J, Berzin I (1995) Distribution of energy dissipation in airlift reactors. Chem Eng Sci 50:2225–2233
Merchuk J, Ronen M, Giris S, Shoshana A (1998) Light/dark cycles in the growth of the red microalga Porphyridium sp. Biotechnol Bioeng 59:705–713
Oncel S, Sukan V (2007) Comparison of two different pneumatically mixed column photobioreactors for the cultivation of Artrospira platensis (Spirulina platensis). Bioresour Technol 99:4755–4760
Radmann E, Radmann C, Reinehr Costa J (2007) Optimization of the repeated batch cultivation of microalgae Spirulina platensis in open raceway ponds. Aquaculture 265:118–126
Sánchez Mirón A, Contreras Gómez A, García Camacho F, Molina Grima E, Chisti Y (1999) Comparative evaluation of compact photobioreactors for large-scale monoculture of microalgae. J Biotechnol 70:249–270
Sánchez Mirón A, Cerón M, García Camacho F, Molina E, Chisti Y (2002) Growth and biochemical characterization of microalgal biomass produced in bubble column and airlift photobioreactors: studies in fed-batch culture. Enzyme Microb Technol 31:1015–1023
Siegel M, Merchuk JC, Schiigerl K, (1986) Gas recirculation in air-lift reactors. A.I.Ch.E.J. 32: 1585
Van Benthum W, van der Lans R, van Loosdrecht M, Heijnen J (1999) Bubble recirculation regimes in an internal airlift loop reactor. Chem Eng Sci 54:3995–4006
Van Harmelen T, Oonk H (2006) Microalgae biofixation processes: applications and potential contributions to greenhouse gas mitigation options. Report, International Network on Biofixation of CO2 and Greenhouse Gas Abatement, the Netherlands
Wallis G (1969) One-dimensional two phase flow. McGraw-Hill, New York
Wongsuchoto P, Pavansant P (2004) Internal liquid circulation in annulus sparged internal loop airlift contactors. Chem Eng J 100:1–9
Zhang X, Baicheng Z, Yiping Z, Zhaoling C, Wei C, Fan O (2002) A simple and low-cost airlift photobioreactor for microalgal mass culture. Biotechnol Lett 24:1767–1771
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The authors thank to the group “Nouvelles Stratégies Energétiques” for financial support.
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Rengel, A., Zoughaib, A., Dron, D. et al. Hydrodynamic study of an internal airlift reactor for microalgae culture. Appl Microbiol Biotechnol 93, 117–129 (2012). https://doi.org/10.1007/s00253-011-3398-9
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DOI: https://doi.org/10.1007/s00253-011-3398-9