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Small RNAs as regulators of primary and secondary metabolism in Pseudomonas species

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Abstract

Small RNAs (sRNAs) exert important functions in pseudomonads. Classical sRNAs comprise the 4.5S, 6S, 10Sa and 10Sb RNAs, which are known in enteric bacteria as part of the signal recognition particle, a regulatory component of RNA polymerase, transfer–messenger RNA (tmRNA) and the RNA component of RNase P, respectively. Their homologues in pseudomonads are presumed to have analogous functions. Other sRNAs of pseudomonads generally have little or no sequence similarity with sRNAs of enteric bacteria. Numerous sRNAs repress or activate the translation of target mRNAs by a base-pairing mechanism. Examples of this group in Pseudomonas aeruginosa are the iron-repressible PrrF1 and PrrF2 sRNAs, which repress the translation of genes encoding iron-containing proteins, and PhrS, an anaerobically inducible sRNA, which activates the expression of PqsR, a regulator of the Pseudomonas quinolone signal. Other sRNAs sequester RNA-binding proteins that act as translational repressors. Examples of this group in P. aeruginosa include RsmY and RsmZ, which are central regulatory elements in the GacS/GacA signal transduction pathway, and CrcZ, which is a key regulator in the CbrA/CbrB signal transduction pathway. These pathways largely control the extracellular activities (including virulence traits) and the selection of the energetically most favourable carbon sources, respectively, in pseudomonads.

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Acknowledgments

This work was supported by the Swiss National Foundation, the Hertha-Firnberg Research fellowship from the Austrian Science Fund (to E.S.) and a genomics project of the University of Lausanne. We thank Cornelia Reimmann and Karine Lapouge for critically reading the manuscript.

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  1. Max F. Perutz Laboratories, Department of Microbiology, Immunobiology and Genetics, University of Vienna, Dr. Bohrgasse 9, 1030, Vienna, Austria

    Elisabeth Sonnleitner

  2. Département de Microbiologie Fondamentale, Université de Lausanne, CH-1015, Lausanne, Switzerland

    Dieter Haas

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Correspondence to Elisabeth Sonnleitner.

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Sonnleitner, E., Haas, D. Small RNAs as regulators of primary and secondary metabolism in Pseudomonas species. Appl Microbiol Biotechnol 91, 63–79 (2011). https://doi.org/10.1007/s00253-011-3332-1

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  • DOI: https://doi.org/10.1007/s00253-011-3332-1

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