Abstract
GlnR is the global regulator of nitrogen assimilation in Streptomyces coelicolor M145 and other actinobacteria. Two-dimensional polyacrylamide gel electrophoresis analyses were performed to identify new GlnR target genes by proteomic comparison of wild-type S. coelicolor M145 and a ΔglnR mutant. Fifty proteins were found to be differentially regulated between S. coelicolor M145 and the ΔglnR mutant. These spots were identified by nanoHPLC–ESI-MS/MS and classified according to their cellular role. Most of the identified proteins are involved in amino acid biosynthesis and in carbon metabolism, demonstrating that the role of GlnR is not restricted to nitrogen metabolism. Thus, GlnR is supposed to play an important role in the global metabolic control of S. coelicolor M145.
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Acknowledgments
Y.T. acknowledges a scholarship from the Studienstiftung des deutschen Volkes. This work was supported by the EU (LSH 4032, ActinoGen) and the BMBF as part of the SYSMO project (5019). The authors wish to thank Hans-Peter Fiedler and Dirk Schulz for their help with actinorhodin extraction, and Silke Wahl, Inga Buchen, and Johannes Madlung for the excellent technical assistance. The Proteome Center Tübingen is supported by the Ministerium für Wissenschaft und Kunst, Landesregierung Baden-Württemberg. We acknowledge Tobias Lamkemeyer for coordinating the proteomic analysis.
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Tiffert, Y., Franz-Wachtel, M., Fladerer, C. et al. Proteomic analysis of the GlnR-mediated response to nitrogen limitation in Streptomyces coelicolor M145. Appl Microbiol Biotechnol 89, 1149–1159 (2011). https://doi.org/10.1007/s00253-011-3086-9
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DOI: https://doi.org/10.1007/s00253-011-3086-9