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Production of rhamnolipids in solid-state cultivation using a mixture of sugarcane bagasse and corn bran supplemented with glycerol and soybean oil

  • Biotechnological Products and Process Engineering
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Abstract

Rhamnolipid biosurfactants are attracting attention due to their low toxicity, high biodegradability, and good ecological acceptability. However, production in submerged culture is made difficult by severe foaming problems. Solid-state cultivation (SSC) is a promising alternative production method. In the current work, we report the optimization of rhamnolipid production by Pseudomonas aeruginosa UFPEDA 614 on a solid substrate containing sugarcane bagasse and corn bran. The best rhamnolipid production, 45 g/l of impregnating solution used, was obtained with a 50:50 (m/m) mixture of sugarcane bagasse and corn bran supplemented with an impregnating solution containing 6% (v/v) of each of glycerol and soybean oil. This level is comparable with those of previous studies undertaken in solid-state cultivation; the composition of the biosurfactant is similar, but our medium is cheaper. Our work therefore provides a suitable basis for future studies of the development of an SSC-based process for rhamnolipid production.

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References

  • Banat IM, Makkar RS, Cameotra SS (2000) Potential commercial applications of microbial surfactants. Appl Microbiol Biotechnol 53:495–508

    Article  CAS  Google Scholar 

  • Benincasa M, Contiero J, Manresa MA, Moraes IO (2002) Rhamnolipid production by Pseudomonas aeruginosa LBI growing on soapstock as the sole carbon source. J Food Eng 54:283–288

    Article  Google Scholar 

  • Bonilla M, Olivaro C, Corona M, Vazquez M, Soubes M (2005) Production and characterization of a new bioemulsifier from Pseudomonas putida ML2. J Appl Microbiol 98:456–463

    Article  CAS  Google Scholar 

  • Bordas F, Lafrance P, Villemur R (2005) Conditions for effective removal of pyrene from an artificially contaminated soil using Pseudomonas aeruginosa 57SJ rhamnolipids. Environ Pollut 138:69–76

    Article  CAS  Google Scholar 

  • Camilios Neto D, Meira JA, Araújo JM, Mitchell DA, Krieger N (2008) Optimization of the production of rhamnolipids by Pseudomonas aeruginosa UFPEDA 614 in solid-state culture. Appl Microbiol Biotechnol 81:441–448

    Article  CAS  Google Scholar 

  • Camilios Neto D, Meira JA, Tiburtius E, Zamora PP, Bugay C, Mitchell DA, Krieger N (2009) Production of rhamnolipids in solid-state cultivation: characterization, downstream processing and application in the cleaning of contaminated soils. Biotechnol J 4:748–755

    Article  CAS  Google Scholar 

  • Costa SGVAO, Nitschke M, Haddad R, Eberlin M, Contiero J (2006) Production of Pseudomonas aeruginosa LBI rhamnolipids following growth on Brazilian native oils. Process Biochem 41:483–488

    Article  CAS  Google Scholar 

  • Desai JD, Banat IM (1997) Microbial production of surfactants and their commercial potential. Microbiol Mol Biol Rev 61:47–64

    CAS  Google Scholar 

  • Domon B, Costello CE (1988) A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugates. Glycoconj J 4:397–409

    Article  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Itoh S, Honda H, Tomota F, Suzuki T (1971) Rhamnolipids produced by Pseudomonas aeruginosa grown on n-paraffin. J Antibiot 24:855–859

    CAS  Google Scholar 

  • Kim SK, Yoon BD, Lee CH, Suh HH, Oh HM, Katsuragi T, Tani Y (1997) Production and properties of a lipopeptide biosurfactant from Bacillus subtilis C9. J Ferment Bioeng 84:41–46

    Article  Google Scholar 

  • Lee BS, Kim EK (2004) Lipopeptide production from Bacillus sp. GB16 using a novel oxygenation method. Enz Microb Technol 35:639–647

    Article  CAS  Google Scholar 

  • Linhardt RJ, Bakhit R, Daniels L, Mayerl F, Pickenhagen W (1989) Microbially produced rhamnolipid as a source of rhamnose. Biotechnol Bioeng 33:365–368

    Article  CAS  Google Scholar 

  • Maier RM, Soberón-Chávez G (2000) Pseudomonas aeruginosa rhamnolipids: biosynthesis and potential applications. Appl Microbiol Biotechnol 54:625–633

    Article  CAS  Google Scholar 

  • Mata-Sandoval JC, Karns J, Torrents A (1999) High-performance liquid chromatography method for the characterization of rhamnolipid mixtures produced by Pseudomonas aeruginosa UG2 on corn oil. J Chromatogr A 864:211–220

    Article  CAS  Google Scholar 

  • Monteiro SA, Sassaki GL, Souza LM, Meira JA, Araújo JM, Mitchell DA, Ramos LP, Krieger N (2007) Molecular and structural characterization of the biosurfactant produced by Pseudomonas aeruginosa DAUPE 614. Chem Phys Lipids 147:1–13

    Article  CAS  Google Scholar 

  • Mulligan CN (2005) Environmental application for biosurfactants. Environ Pollut 133:183–198

    Article  CAS  Google Scholar 

  • Mulligan CN, Gibbs BF (1993) Factors influencing the economics of biosurfactants. In: Kosaric N (ed) Biosurfactants. Marcel Dekker, New York, pp 329–371

    Google Scholar 

  • Sim L, Ward OP, Li Z-Y (1997) Production and characterisation of a biosurfactant isolated from Pseudomonas aeruginosa UW-1. J Ind Microbiol Biotechnol 19:232–238

    Article  CAS  Google Scholar 

  • Souza LM, Cipriani TR, Serrato RV, Costa DE, Iacomini M, Gorin PAJ, Sassaki GL (2008) Analysis of flavonol glycoside isomers from leaves of Maytenus ilicifolia by offline and online high performance liquid chromatography–electrospray mass spectrometry. J Chromatogr A 1207:101–109

    Article  Google Scholar 

  • Souza LM, Cipriani TR, Sant’Ana CF, Iacomini M, Gorin PAJ, Sassaki GL (2009) Heart-cutting two-dimensional (size exclusion × reversed phase) liquid chromatography–mass spectrometry analysis of flavonol glycosides from leaves of Maytenus ilicifolia. J Chromatogr A 1216:99–105

    Article  Google Scholar 

  • Trummler K, Effenberger F, Syldatk C (2003) An integrated microbial/enzymatic process for production of rhamnolipids and l-(+)-rhamnose from rapeseed oil with Pseudomonas sp. DSM 2874. Eur J Lipid Sci Technol 105:563–571

    Article  CAS  Google Scholar 

  • Veenanadig NK, Gowthaman MK, Karanth NGK (2000) Scale up studies for the production of biosurfactant in packed column bioreactor. Bioprocess Eng 22:95–99

    Article  CAS  Google Scholar 

  • Yeh MS, Wei TH, Chang JS (2006) Bioreactor design for enhanced carrier-assisted surfactin production with Bacillus subtilis. Process Biochem 41:1799–1805

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported financially by Corn Products Brasil and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil), a Brazilian government agency for the advancement of science and technology. Research scholarships were granted to David Mitchell, Nadia Krieger, Arquimedes Paixão de Santana-Filho, Guilherme L. Sassaki, and Lauro M. de Souza by CNPq, to Doumit Camilios-Neto by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil), and to Cryshelen Bugay and Talita Joslin by Corn Products Brasil.

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Authors and Affiliations

  1. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx.P. 19046 Centro Politécnico, Curitiba, 81531-980, Paraná, Brazil

    Doumit Camilios-Neto, Arquimedes Paixão de Santana-Filho, Lauro Mera de Souza, Guilherme Lanzi Sassaki & David Alexander Mitchell

  2. Departamento de Química, Universidade Federal do Paraná, Cx.P. 19081 Centro Politécnico, Curitiba, 81531-980, Paraná, Brazil

    Cryshelen Bugay, Talita Joslin & Nadia Krieger

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  1. Doumit Camilios-Neto
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  2. Cryshelen Bugay
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  3. Arquimedes Paixão de Santana-Filho
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  4. Talita Joslin
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  5. Lauro Mera de Souza
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  6. Guilherme Lanzi Sassaki
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  7. David Alexander Mitchell
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  8. Nadia Krieger
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Correspondence to Nadia Krieger.

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Camilios-Neto, D., Bugay, C., de Santana-Filho, A.P. et al. Production of rhamnolipids in solid-state cultivation using a mixture of sugarcane bagasse and corn bran supplemented with glycerol and soybean oil. Appl Microbiol Biotechnol 89, 1395–1403 (2011). https://doi.org/10.1007/s00253-010-2987-3

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  • DOI: https://doi.org/10.1007/s00253-010-2987-3

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