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The gld1 + gene encoding glycerol dehydrogenase is required for glycerol metabolism in Schizosaccharomyces pombe

  • Applied Microbial and Cell Physiology
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Abstract

The budding yeast Saccharomyces cerevisiae is able to utilize glycerol as the sole carbon source via two pathways (glycerol 3-phosphate pathway and dihydroxyacetone [DHA] pathway). In contrast, the fission yeast Schizosaccharomyces pombe does not grow on media containing glycerol as the sole carbon source. However, in the presence of other carbon sources such as galactose and ethanol, S. pombe could assimilate glycerol and glycerol was preferentially utilized over ethanol and galactose. No equivalent of S. cerevisiae Gcy1/glycerol dehydrogenase has been identified in S. pombe. However, we identified a gene in S. pombe, SPAC13F5.03c (gld1 +), that is homologous to bacterial glycerol dehydrogenase. Deletion of gld1 caused a reduction in glycerol dehydrogenase activity and prevented glycerol assimilation. The gld1Δ cells grew on 50 mM DHA as the sole carbon source, indicating that the glycerol dehydrogenase encoded by gld1 + is essential for glycerol assimilation in S. pombe. Strains of S. pombe deleted for dak1 + and dak2 + encoding DHA kinases could not grow on glycerol and showed sensitivity to a higher concentration of DHA. The dak1Δ strain showed a more severe reduction of growth on glycerol and DHA than the dak2Δ strain because the expression of dak1 + mRNA was higher than that of dak2 +. In wild-type S. pombe, expression of the gld1 +, dak1 +, and dak2 + genes was repressed at a high concentration of glucose and was derepressed during glucose starvation. We found that gld1 + was regulated by glucose repression and that it was derepressed in scr1Δ and tup12Δ strains.

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Acknowledgments

We thank Dr. Yukio Mukai for the fission yeast strains. This work was partly supported by the Project for Development of a Technological Infrastructure for Industrial Bioprocesses on R&D of New Industrial Science and Technology Frontiers by the Ministry of Economy, Trade & Industry (METI), as supported by the New Energy and Industrial Technology Development Organization (NEDO).

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Authors and Affiliations

  1. Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa, 761-0795, Japan

    Tomohiko Matsuzawa, Akira Hosomi, Naotaka Tanaka & Kaoru Takegawa

  2. Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan

    Takao Ohashi & Kaoru Takegawa

  3. ASPEX Division, Research Center, Asahi Glass Co., Ltd., 1150 Hazawa-cho, Kanagawa-ku, Yokohama, 221-8755, Japan

    Takao Ohashi & Hideki Tohda

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  1. Tomohiko Matsuzawa
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  2. Takao Ohashi
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  3. Akira Hosomi
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  4. Naotaka Tanaka
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  6. Kaoru Takegawa
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Correspondence to Kaoru Takegawa.

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Matsuzawa, T., Ohashi, T., Hosomi, A. et al. The gld1 + gene encoding glycerol dehydrogenase is required for glycerol metabolism in Schizosaccharomyces pombe . Appl Microbiol Biotechnol 87, 715–727 (2010). https://doi.org/10.1007/s00253-010-2586-3

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