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The inhibitory effects of mushroom extracts on sucrose-dependent oral biofilm formation

  • Biotechnologically Relevant Enzymes and Proteins
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Abstract

Mushrooms contain large quantities of α-glucans. Shiitake (Lentinula edodes), Japan’s most popular edible mushroom, has been reported to contain about 6% (weight/dried weight) of α-(1,3)-glucan. This glucan is one of the major components of oral biofilm formed by the cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. We found that extracts from shiitake and other edible mushrooms could reduce preformed biofilms of S. mutans and S. sobrinus in the presence of dextranase. We also investigated the α-glucanase activities of shiitake mushroom extracts and their effects on biofilm formation. The extracts possessed α-glucanase activity and degraded water-insoluble glucans from mutans streptococci. The extracts strongly inhibited the sucrose-dependent formation of biofilms by S. mutans and S. sobrinus in the presence of dextranase. Our results suggest that some components of mushrooms, including α-glucanases, might inhibit the sucrose-induced formation of oral biofilms.

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Acknowledgements

This study was supported in part by Grants-in-Aid for Scientific Research numbers 21792165 and 20592181, and for High-Tech Research Projects (2009–2010 and 2005–2009, respectively) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate, 024-0003, Japan

    Akira Yano, Sayaka Kikuchi, Yuichi Sakamoto & Yuko Nakagawa

  2. Department of Preventive Dentistry, Kyushu University Faculty of Dentistal Science, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Yoshihisa Yamashita

  3. Department of Dental Pharmacology, Iwate Medical University School of Dentistry, 1-3-27 Chuo-dori, Morioka, Iwate, 020-8505, Japan

    Yasuo Yoshida

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  1. Akira Yano
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  2. Sayaka Kikuchi
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  3. Yoshihisa Yamashita
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  4. Yuichi Sakamoto
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  5. Yuko Nakagawa
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  6. Yasuo Yoshida
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Correspondence to Akira Yano.

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Yano, A., Kikuchi, S., Yamashita, Y. et al. The inhibitory effects of mushroom extracts on sucrose-dependent oral biofilm formation. Appl Microbiol Biotechnol 86, 615–623 (2010). https://doi.org/10.1007/s00253-009-2323-y

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  • DOI: https://doi.org/10.1007/s00253-009-2323-y

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