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Microbial community of acetate utilizing denitrifiers in aerobic granules

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Abstract

The aerobic sludge granules cultivated at high organic loading rates could effectively convert 100–700 mg l−1 nitrite to nitrogen gas with 400 or 1,200 mg l−1 dosed acetate. The denitrifying microbial community structure of the so-cultivated granules was investigated by 16S rRNA gene sequences and localized using fluorescence in situ hybridization (FISH). The 16S rRNA gene phylotypes in the clone library and FISH probes used exhibited high diversity among the bacteria and denitrifying communities, with the members of Betaproteobacteria predominant that were closely related to families Comamonadaceae, Nitrosomonadaceae, Alcaligenaceae, and Rhodocyclaceae. The confocal laser scanning microscope and staining test revealed that active microbial community principally distributed at 200–250 μm beneath the outer surface, embedded in extracellular polymeric substances.

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References

  • Adav SS, Lee DJ, Lai JY (2009) Biological nitrification–denitrification with alternating oxic and anoxic operation using aerobic granules. Appl Microbiol Biotechnol. doi:10.1007/s00253-009-2129-y

  • Adav SS, Chen MY, Lee DJ, Ren NQ (2007) Degradation of phenol by aerobic granules and isolated yeast Candida tropicalis. Biotechnol Bioeng 96:844–852

    Article  CAS  Google Scholar 

  • Adav SS, Lee DJ, Show KY, Tay JH (2008) Aerobic granular sludge: Recent advances. Biotechnol Adv 26:411–423

    Article  CAS  Google Scholar 

  • Amann RI, Binder BJ, Olson RJ, Chisholm SW, Devereux R, Stahl DA (1990) Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol 56:1919–1925

    CAS  Google Scholar 

  • APHA (1998) Standard methods for the examination of water and wastewater, 20th edn. American Public Health Association, Washington

    Google Scholar 

  • Beun JJ, Van Loosdrecht MCM, Heijnen JJ (2001) N-removal in a granular sludge sequencing batch airlift reactor. Biotechnol Bioeng 75:82–92

    Article  CAS  Google Scholar 

  • de Kreuk MK, Heijnen JJ, van Loosdrecht MC (2005) Simultaneous COD, nitrogen, and phosphate removal by aerobic granular sludge. Biotechnol Bioeng 90:761–769

    Article  Google Scholar 

  • Drysdale GD, Kasan HC, Bux F (2001) Assessment of denitrification by the ordinary heterotrophic organisms in an NDBEPR activated sludge system. Water Sci Technol 43:147–154

    CAS  Google Scholar 

  • Etchebehere C, Errazquin I, Barrandeguy E, Dabert P, Moletta R, Muxi L (2001) Evaluation of the denitrifying microbiota of anoxic reactors. FEMS Microbiol Ecol 35:259–265

    Article  CAS  Google Scholar 

  • Etchebehere C, Errazquin MI, Dabert P, Muxi L (2002) Community analysis of a denitrifying reactor treating landfill leachate. FEMS Microbiol Ecol 40:97–106

    Article  CAS  Google Scholar 

  • Ginige MP, Bowyer JC, Foley L, Keller J, Yuan Z (2009) A comparative study of methanol as a supplementary carbon source for enhancing denitrification in primary and secondary anoxic zones. Biodegradation 20:221–234

    Article  CAS  Google Scholar 

  • Ginige MP, Jurg K, Blackall LL (2005) Investigation of an acetate-fed denitrifying microbial community by stable isotope probing, full-cycle rRNA analysis, and fluorescent in situ hybridization-microautoradiography. Appl Environ Microbiol 71:8683–8691

    Article  CAS  Google Scholar 

  • Glass C, Silversterin JA, Oh J (1997) Inhibition of denitrification in activated sludge by nitrite. Water Environ Res 69:1086–1093

    Article  CAS  Google Scholar 

  • Grabinskaloniewska A (1991) Denitrification unit biocenosis. Water Res 25:1565–1573

    Article  CAS  Google Scholar 

  • Khan ST, Hiraishi A (2002) Diaphorobacter nitroreducens gen. nov., sp nov., a poly(3-hydroxybutyrate) degrading denitrifying bacterium isolated from activated sludge. J Gen Appl Microbiol 48:299–308

    Article  CAS  Google Scholar 

  • Khan ST, Horiba Y, Yamamoto M, Hiraishi A (2002) Members of the family Comamonadaceae as primary poly(3-hydroxybutyrate-co-3-hydroxyvalerate)- degrading denitrifiers in activated sludge as revealed by a polyphasic approach. Appl Environ Microbiol 68:3206–3214

    Article  CAS  Google Scholar 

  • Kim H, Vanparys B, Wittebolle L, Verstraete W, Boon N, Vos PD (2006) Cultivation of denitrifying bacteria: optimization of isolation conditions and diversity study. Appl Environ Microbiol 72:2637–2643

    Article  Google Scholar 

  • Liu Y, Tay JH (2004) State of the art of biogranulation technology for wastewater treatment. Biotechnol Adv 22:533–563

    Article  CAS  Google Scholar 

  • Mellor RB, Ronnennberg J, Campbell HW (1992) Reduction of nitrate and nitrite in water by immobilized enzymes. Nature 355:717–719

    Article  CAS  Google Scholar 

  • Mills HJ, Hunter E, Humphrys M, Kerkhof L, McGuinness L, Huettel M, Kostka JE (2008) Characterization of nitrifying, denitrifying, and overall bacterial communities in permeable marine sediments of the northeastern Gulf of Mexico. Appl Environ Microbiol 74:4440–4453

    Article  CAS  Google Scholar 

  • Mosquera-Corral A, de Kreuk MK, Heijnen JJ, van Loosdrecht MCM (2005) Effects of oxygen concentration on N-removal in an aerobic granular sludge reactor. Water Res 39:2676–2686

    Article  CAS  Google Scholar 

  • Moy BYP, Tay JH, Toh SK, Liu Y, Tay STL (2002) High organic loading influences the physical characteristics of aerobic sludge granules. Lett Appl Microbiol 34:407–412

    Article  Google Scholar 

  • Otlanabo NL (1993) Denitrification of ground water for potable purposes. WRC Report No. 403/1/93.

  • Park HI, Choi YJ, Pak D (2005) Autohydrogenotrophic denitrifying microbial community in a glass beads biofilm reactor. Biotechnol Lett 27:949–953

    Article  CAS  Google Scholar 

  • Polz MF, Cavanaugh CM (1998) Bias in template-to-product ratios in multitemplate PCR. Appl Environ Microbiol 64:3724–3730

    CAS  Google Scholar 

  • Rabus R, Wilkes H, Schramm A, Harms G, Behrends A, Amann R, Widdel F (1999) Anaerobic utilization of alkylbenzenes and n-alkanes from crude oil in an enrichment culture of denitrifying bacteria affiliating with the beta-subclass of Proteobacteria. Environ Microbiol 1:145–157

    Article  CAS  Google Scholar 

  • Shapeligh JP, Davies KJP, Payne WJ (1987) Detergent inhibition of nitric oxide reductase. Biochim Biophys Acta 911:334–340

    Google Scholar 

  • Tsikas D, Rossa S, Sandmann J, Frolich JC (1999) High-performance liquid chromatographic analysis of nitrite and nitrate in human plasma as S-nitroso-N-acetylcysteine with ultraviolet absorbance detection. J Chromatogr B Biomed Sci Appl 724:199–201

    Article  CAS  Google Scholar 

  • Tsuneda S, Nagano T, Hoshino T, Ejiri Y, Noda N, Hirata A (2003) Characterization of nitrifying granules produced in an aerobic upflow fluidized bed reactor. Water Res 37:4965–73

    Article  CAS  Google Scholar 

  • Wagner M, Loy A (2002) Bacterial community composition and function in sewage treatment systems. Current Opin Biotechnol 13:218–227

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This project is financially supported by the State Key Laboratory of Water Resource and Environment (SKLWRE) and Harbin Institute of Technology (HIT), China

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Authors and Affiliations

  1. State Key Laboratory of Water Resource and Environment (SKLWRE), Harbin Institute of Technology, Harbin, 150090, China

    Sunil S. Adav & Duu-Jong Lee

  2. Department of Chemical Engineering, National Taiwan University, Taipei, 10617, Taiwan

    Sunil S. Adav & Duu-Jong Lee

  3. Center of Membrane Technology, Department of Chemical Engineering, Chung Yuan Christian University, Chungli, Taiwan

    J. Y. Lai

Authors
  1. Sunil S. Adav
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  2. Duu-Jong Lee
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  3. J. Y. Lai
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Correspondence to Duu-Jong Lee.

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Adav, S.S., Lee, DJ. & Lai, J.Y. Microbial community of acetate utilizing denitrifiers in aerobic granules. Appl Microbiol Biotechnol 85, 753–762 (2010). https://doi.org/10.1007/s00253-009-2263-6

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  • DOI: https://doi.org/10.1007/s00253-009-2263-6

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