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Two-phase partitioning bioreactors in environmental biotechnology

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Abstract

Two-phase partitioning bioreactors (TPPBs) in environmental biotechnology are based on the addition of a non-aqueous phase (NAP) into a biological process in order to overcome both mass-transfer limitations from the gas to aqueous phase and pollutant-mediated inhibitions. Despite constituting a robust and reliable technology in terms of pollutant biodegradation rates and process stability in wastewater, soil, and gas treatment applications, this superior performance only applies for a restricted number of pollutants or contamination events. Severe limitations such as high energy requirements, high costs of some NAPs, foaming, or pollutant sequestration challenge the full-scale application of this technology. The introduction of solid NAPs into this research field has opened a promising pathway for the future development of TPPBs. Finally, this work reviews fundamental aspects of NAP selection and mass transfer and identifies the niches for future research: low energy-demand bioreactor designs, experimental determination of partial mass transfers, and solid NAP tailoring.

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References

  • Aguila-Hernandez J, Trejo A, García-Flores BE (2007) Surface tension and foam behavior of aqueous solutions of blends of three alkanolamines, as a function of temperature. Colloids Surf A 308:33–46

    Article  CAS  Google Scholar 

  • Alagapan G, Cowan RM (2004) Effect of temperature and dissolved oxygen on the growth kinetics of Pseudomonas putida F1 growing on benzene and toluene. Chemosphere 54:1255–1265

    Article  CAS  Google Scholar 

  • Alper E, Wichtendahl B, Deckwer W (1980) Gas-absorption mechanism in catalytic slurry reactors. Chem Eng Sci 35:217–222

    Article  CAS  Google Scholar 

  • Amsden BG, Bochanysz J, Daugulis AJ (2003) Degradation of xenobiotics in a partitioning bioreactor in which the partitioning phase is a polymer. Biotechnol Bioeng 84:399–405

    Article  CAS  PubMed  Google Scholar 

  • Arriaga S, Muñoz R, Hernandez S, Guieysse B, Revah S (2006) Gaseous hexane biodegradation by fusarium solani in two liquid phase packed-bed and stirred-tank bioreactors. Environ Sci Technol 40:2390–2395

    Article  CAS  PubMed  Google Scholar 

  • Ascon-Cabrera MA, Lebeault JM (1993) Selection of xenobiotic-degrading microorganisms in a biphasic aqueous-organic system. Appl Environ Microbiol 59:1136–1141

    Article  Google Scholar 

  • Ascon-Cabrera MA, Lebeault JM (1995) Interfacial area effects of a biphasic aqueous/organic system of growth kinetic of xenobiotic-degrading microorganisms. Appl Microbiol Biotechnol 43:1136–1141

    Article  CAS  Google Scholar 

  • Bailon L, Nikolausz M, Kastner M, Veiga MC, Kennes C (2009) Removal of dichloromethane form waste gases in one- and two-liquid-phase stirred tank bioreactors and biotrickling filters. Water Res 43:11–20

    Article  CAS  PubMed  Google Scholar 

  • Boudreau NG, Daugulis AJ (2006) Transient Performance of Two phase partitioning bioreactor treating a toluene contaminated gas stream. Biotechnol Bioeng 94:448–457

    Article  CAS  PubMed  Google Scholar 

  • Bruce LJ, Daugulis AJ (1991) Solvent selection strategies for extractive biocatalysis. Biotechnol Prog 7:116–124

    Article  CAS  PubMed  Google Scholar 

  • Bruining WJ, Joosten GEH, Beenackers AACM, Hofman H (1986) Enhancement of gas-liquid mass transfer by a dispersed second liquid phase. Chem Eng Sci 41:1873–1877

    Article  CAS  Google Scholar 

  • Cerniglia CE (1993) Biodegradation of polycyclic aromatic hydrocarbons. Curr Opin Biotechnol 4:331–338

    Article  CAS  Google Scholar 

  • Cesario MT, Beeftink HH, Tramper J (1992) Biological treatment of waste gases containing poorly-water soluble compounds. In: Dragt AJ, van Ham J (eds) Biotechniques for air pollution abatements and odour control policies. Elsevier Science Publishers, Amsterdam, pp 135–140

    Google Scholar 

  • Cesario MT, Turtoi M, Sewalt SFM, Beeftink HH, Tramper J (1996) Enhancement of the gas-to-water ethene transfer coefficient by a dispersed water-immiscible solvent: effect of the cells. Appl Microbiol Biotechnol 46:497–502

    Article  CAS  Google Scholar 

  • Cesario MT, Beverloo WA, Tramper J, Beeftink HH (1997a) Enhancement of gas-liquid mass transfer rate of apolar pollutants in the biological waste gas treatment by a dispersed organic solvent. Enzyme Microb Tech 21:578–588

    Article  CAS  Google Scholar 

  • Cesario MT, Beverloo WA, Tramper J, Beeftink HH (1997b) Enhancement of gas-liquid mass transfer rate of apolar pollutants in the biological waste gas treatment by a dispersed organic solvent. Enzyme Microb Technol 21:578–588

    Article  CAS  Google Scholar 

  • Cesario MT, Brandsma JB, Boon MA, Tramper J, Beeftink HH (1998) Ethene removal from gas by recycling a water-immiscible solvent through a packed absorber and a bioreactor. J Biotech 62:105–118

    Article  CAS  Google Scholar 

  • Clarke KG, Correia LDC (2008) Oxygen transfer in hydrocarbon-aqueous dispersions and its applicability to alkane bioprocesses: A review. Biochem Eng J 39:405–429

    Article  CAS  Google Scholar 

  • Clarke KG, Williams PC, Smit MS, Harrison STL (2006) Enhancement and repression of the volumetric oxygen transfer coefficient through hydrocarbon addition and its influence on oxygen transfer rate in stirred tank bioreactors. Biochem Eng J 28:237–242

    Article  CAS  Google Scholar 

  • Collins LD, Daugulis AJ (1997) Biodegradation of phenol at high initial concentrations in two-phase partitioning batch and fed-batch bioreactors. Biotechnol Bioeng 55:155–162

    Article  CAS  PubMed  Google Scholar 

  • Collins LD, Daugulis AJ (1999a) Benzene/toluene/p-xylene degradation. Part II. Effect of substrate interactions and feeding strategies in toluene/benzene and toluene/p-xylene fermentations in a partitioning bioreactor. Appl Microbiol Biotechnol 52:360–365

    Article  CAS  PubMed  Google Scholar 

  • Collins LD, Daugulis AJ (1999b) Simultaneous biodegradation of benzene, toluene, and p-xylene in a two-phase partitioning bioreactor: concept demonstration and practical application. Biotechnol Prog 15:74–80

    Article  CAS  PubMed  Google Scholar 

  • Cordova-Rosa SM, Dams RI, Cordova-Rosa EV, Radetski MR, Correa AXR, Radetski CM (2009) Remediation of phenol-contaminated soil by a bacterial consortium and Acinetobacter calcoaceticus isolated from an industrial wastewater treatment plant. J Hazard Mater 164:61–66

    Article  CAS  PubMed  Google Scholar 

  • Cruickshank SM, Daugulis AJ, McLellan PJ (2000) Dynamic modeling and optimal fed-batch feeding strategies for a two-phase partitioning bioreactor. Biotechnol Bioeng 67:224–233

    Article  CAS  PubMed  Google Scholar 

  • Daugulis AJ (1997) Partitioning bioreactors. Curr Opin Biotechnol 8:169–174

    Article  CAS  PubMed  Google Scholar 

  • Daugulis AJ (2001) Two-phase partitioning bioreactors: a new technology platform for destroying xenobiotics. Trends Biotechnol 19:457–462

    Article  CAS  PubMed  Google Scholar 

  • Daugulis AJ, Boudreau NG (2003) Removal and destruction of high concentrations of gaseous toluene in a two- phase partitioning bioreactor by Alcaligenes xylosoxidans. Biotechnol Lett 25:1421–1424

    Article  CAS  PubMed  Google Scholar 

  • Daugulis AJ, Boudreau NG (2008) Solid -liquid two phase partitioning bioreactors for the treatment of gas-phase volatile organic carbons by a microbial consortium. Biotechnol Lett 30:1583–1587

    Article  CAS  PubMed  Google Scholar 

  • Daugulis AJ, Janikowski TB (2002) Scale-up performance of a partitioning bioreactor for the degradation of polyaromatic hydrocarbons by Sphingomonas aromaticivorans. Biotechnol Lett 24:591–594

    Article  CAS  Google Scholar 

  • Daugulis AJ, Amsden B, Bochanysz J, Kayssi A (2003) Delivery of benzene to Alcaligenes xylosoxidans by solid polymers in a two-phase partitioning bioreactor. Biotechnol Lett 25:1203–1207

    Article  CAS  PubMed  Google Scholar 

  • Davidson CT, Daugulis AJ (2003a) The treatment of gaseous benzene by two-phase partitioning bioreactors: a high performance alternative to the use of biofilters. Appl Microbiol Biotechnol 62:297–301

    Article  CAS  PubMed  Google Scholar 

  • Davidson CT, Daugulis AJ (2003b) Addressing biofilter limitations: A two phase partitioning bioreactor process for the treatment of benzene and toluene contaminated gas streams. Biodegradation 14:415–421

    Article  CAS  PubMed  Google Scholar 

  • Deziel E, Comeau Y, Villemur R (1999) Two-liquid-phase bioreactors for enhanced biodegradation of hydrophobic/toxic compounds. Biodegradation 10:219–233

    Article  CAS  PubMed  Google Scholar 

  • Dumont E, Delmas H (2003) Mass transfer enhancement of gas absorption in oil-in-water systems: a review. Chem Eng Process 42:419–438

    Article  CAS  Google Scholar 

  • Dumont E, Andres Y, Le cloirec P (2005) Enhancement of oxygen transfer in bioprocesses by the use of an organic phase: Effect of silicone oil on volumetric mass transfer coefficient of oxygen (k La). In: Kennes C, Veiga MC (eds) Proceedings of the international congress biotechniques for air pollution control. pp 163–173. A Coruña, Spain

  • Dumont E, Andres Y, Le Cloirec P (2006a) Mass transfer coefficients of styrene and oxygen into silicone oil emulsions in a bubble reactor. Chem Eng Sci 61:5612–5619

    Article  CAS  Google Scholar 

  • Dumont E, Andres Y, Le Cloirec P (2006b) Effect of organic solvents on oxygen mass transfer in multiphase systems: application to bioreactors in environmental protection. Biochem Eng J 30:245–252

    Article  CAS  Google Scholar 

  • Fazaelipoor MH (2007) A model for treating air streams in a continuous two liquid phase stirred tank bioreactor. J Hazard Mater 148:453–458

    Article  CAS  PubMed  Google Scholar 

  • Fazaelipoor MH, Shojaosadati SA (2002) The effect of silicone oil on biofiltration of hydrophobic compounds. Environ Prog 21:221–224

    Article  CAS  Google Scholar 

  • Galindo E, Pacek AW, Nienow AW (2000) Study of drop and bubble sizes in a simulated mycelial fermentation broth of up to four phases. Biotechnol Bioeng 69:213–221

    Article  CAS  PubMed  Google Scholar 

  • Gardin H, Lebeault JM, Pauss A (1999) Biodegradation of xylene and butyl acetate using an aqueous-silicon oil two-phase system. Biodegradation 10:193–200

    Article  CAS  PubMed  Google Scholar 

  • Guieysse B (2001) Inovative bioreactors for the degradation of polycyclic aromatic hydrocarbons. PhD Thesis. Lund University

  • Guieysse B, Viklund G (2005) Sequential UV-biological degradation of polycyclic aromatic hydrocarbons in two-phases partitioning bioreactors. Chemosphere 59:369–376

    Article  CAS  PubMed  Google Scholar 

  • Guieysse B, Cirne MDTG, Mattiasson B (2001) Microbial degradation of phenanthrene and pyrene in a two-liquid phase-partitioning bioreactor. Appl Microbiol Biotechnol 56:796–802

    Article  CAS  PubMed  Google Scholar 

  • Guieysse B, Autem Y, Soares A (2005) Biodegradation of phenol at low temperature using two-phase partitioning bioreactors. Water Sci Technol 52:97–105

    Article  CAS  PubMed  Google Scholar 

  • Hamed TA, Bayraktar E, Mehmetoglu U, Mehmetoglu T (2004) The biodegradation of benzene, toluene and phenol in a two-phase system. Biochem Eng J 19:137–146

    Article  CAS  Google Scholar 

  • Hejazi RF, Husain T (2004) Landfarm performance under arid conditions. 1. Conceptual framework. Environ Sci Technol 38:2449–2456

    Article  CAS  PubMed  Google Scholar 

  • Ho CS, Ju LK, Baddour RF (1990) Enhancing penicillin fermentations by increased oxygen solubility through the addition of n-hexadecane. Biotechnol Bioeng 36:1110–1118

    Article  CAS  PubMed  Google Scholar 

  • Janikowski TB, Velicogna D, Punt M, Daugulis AJ (2002) Use of a two-phase partitioning bioreactor for degrading polycyclic aromatic hydrocarbons by a Sphingomonas sp. Appl Microbiol Biotechnol 59:368–376

    Article  CAS  PubMed  Google Scholar 

  • Jia S, Li P, Park YS, Okabe M (1996) Enhanced oxygen transfer in tower bioreactor on addition of liquid hydrocarbons. J Ferment Bioeng 82:191–193

    Article  CAS  Google Scholar 

  • Jorgensen A, Giessing AMB, Rasmussen LJ, Andersen O (2008) Biotransformation of polycyclic aromatic hydrocarbons in marine polychaetes. Mar Environ Res 65:171–186

    Article  CAS  PubMed  Google Scholar 

  • Ju LK, Ho CS (1989) Oxygen diffusion coefficient and solubility in n-hexadecane. Biotechnol Bioeng 34:1221–1224

    Article  CAS  PubMed  Google Scholar 

  • Kars R, Best R, Drinkenburg A (1979) Sorption of propane in slurries of active carbon in water. Chem Eng Jour 17:201–210

    Article  CAS  Google Scholar 

  • Kim IS, Park JS, Kim KW (2001) Enhanced biodegradation of polycyclic aromatic hydrocarbons using nonionic surfactants in soil slurry. Appl Geochem 16:1419–1428

    Article  CAS  Google Scholar 

  • Kohler A, Schuttoff M, Bryniok D, Knackmuss HJ (1994) Enhanced biodegradation of phenanthrene in a biphasic culture system. Biodegradation 5:93–103

    Article  Google Scholar 

  • Lei L, Suidan MT, Khodadoust AP, Tabak HH (2004) Assessing the bioavailability of PAHs in field-contaminated sediment using XAD-2 assisted desorption. Environ Sci Technol 38:1786–1793

    Article  CAS  PubMed  Google Scholar 

  • Littlejohns JV, Daugulis AJ (2007) Oxygen transfer in a gas–liquid system containing solids of varying oxygen affinity. Chem Eng J 129:67–74

    Article  CAS  Google Scholar 

  • Littlejohns JV, Daugulis AJ (2008) Response of a solid-liquid two-phase partitioning bioreactor to transient BTEX loadings. Chemosphere 73:1453–1460

    Article  CAS  PubMed  Google Scholar 

  • Littlejohns JV, Daugulis AJ (2009) A two-phase partitioning airlift bioreactor for the treatment of BTEX contaminated gases. Biotechnol Bioeng 103:1077–1086

    Article  CAS  PubMed  Google Scholar 

  • MacLeod CT, Daugulis AJ (2003) Biodegradation of polycyclic aromatic hydrocarbons in a two-phase partitioning bioreactor in the presence of a bioavailable solvent. Appl Microbiol Biotechnol 62:291–296

    Article  CAS  PubMed  Google Scholar 

  • MacLeod CT, Daugulis AJ (2005) Interfacial effects in a two-phase partitioning bioreactor: degradation of polycyclic aromatic hydrocarbons (PAHs) by a hydrophobic Mycobacterium. Process Biochem 40:1799–1805

    Article  CAS  Google Scholar 

  • Mahanty B, Pakshirajan K, Dasu VV (2008) Biodegradation of pyrene by Mycobacterium frederiksbergense in a two-phase partitioning bioreactor system. Bioresour Technol 99:2694–2698

    Article  CAS  PubMed  Google Scholar 

  • Malinowski JJ (2001) Two-phase partitioning bioreactors in fermentation technology. Biotechnol Adv 19:525–538

    Article  CAS  PubMed  Google Scholar 

  • Mancera-Lopez ME, Esparza-Garcia F, Chavez-Gomez B, Rodriguez-Vazquez R, Saucedo-Castañeda G, Barrera-Cortes J (2008) Bioremediation of an aged hydrocarbon-contaminated soil by a combined system of biostimulation-bioaugmentation with filamentous fungi. Int Biodeterior Biodegrad 61:151–160

    Article  CAS  Google Scholar 

  • Marcoux J, Deziel E, Villemur R, Lepine F, Bisaillon JG, Beaudet R (2000) Optimization of high-molecular-weight polycyclic aromatic hydrocarbons' degradation in a two-liquid-phase bioreactor. J Appl Microbiol 88:655–662

    Article  CAS  PubMed  Google Scholar 

  • Mileski GJ, Bumpus JA, Jurek MA, Aust SD (1988) Biodegradation of pentachlorophenol by the white rot fungus Phanerochaete chrysosporium. Appl Environ Microbiol 54:2885–2889

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mohan SV, Prasanna D, Reddy BP, Sarma PN (2008) Ex situ bioremediation of pyrene contaminated soil in bio-slurry phase reactor operated in periodic discontinuous batch mode: Influence of bioaugmentation. Int Biodeterior Biodegrad 62:162–169

    Article  CAS  Google Scholar 

  • Mohan SV, Reddy BP, Sarma PN (2009) Ex situ slurry phase bioremediation of chrysene contaminated soil with the function of metabolic function: process evaluation by data enveloping analysis (DEA) and Taguchi design of experimental methodology (DOE). Bioresour Technol 100:164–172

    Article  CAS  Google Scholar 

  • Morrish JLE, Daugulis AJ (2008) Improved reactor performance and operability in the biotransformation of carveol to carvone using a solid–liquid two-phase partitioning bioreactor. Biotechnol Bioeng 101:946–956

    Article  CAS  PubMed  Google Scholar 

  • Muñoz R, Guieysse B, Mattiasson B (2003) Phenanthrene biodegradation by an algal-bacterial consortium in two-phase partitioning bioreactors. Appl Microbiol Biotechnol 61:261–267

    Article  PubMed  CAS  Google Scholar 

  • Muñoz R, Rolvering B, Guieysse B, Mattiasson B (2005) Aerobic phenanthrene biodegradation in a two-phase partitioning bioreactor. Water Sci Technol 52:265–271

    Article  PubMed  Google Scholar 

  • Muñoz R, Arriaga S, Hernandez S, Guieysse B, Revah S (2006) Enhanced hexane biodegradation in a two phase partitioning bioreactor: Overcoming pollutant transport limitations. Process Biochem 41:1614–1619

    Article  CAS  Google Scholar 

  • Muñoz R, Villaverde S, Guieysse B, Revah S (2007a) Two partitioning bioreactors for treatment of volatile organic compounds. Biotechnol Adv 25:410–422

    Article  PubMed  CAS  Google Scholar 

  • Muñoz R, Diaz LF, Bordel S, Villaverde S (2007b) Inhibitory effects of catechol accumulation on benzene biodegradation in Pseudomonas putida F1 cultures. Chemosphere 68:244–252

    Article  PubMed  CAS  Google Scholar 

  • Muñoz R, Chambaud M, Bordel S, Villaverde S (2008) A systematic selection of the non-aqueous phase in a bacterial two liquid phase bioreactor treating α-pinene. Appl Microbiol Biotechnol 79:33–41

    Article  PubMed  CAS  Google Scholar 

  • Muñoz R, Hernández M, Segura A, Gouveia J, Rojas A, Ramos JL, Villaverde S (2009) Continuous cultures of Pseudomonas putida mt-2 overcome catabolic function loss under real case operating conditions. Appl Microbiol Biotechnol 83:189–198

    Article  PubMed  CAS  Google Scholar 

  • Nielsen DR, Daugulis AJ, McLellan PJ (2003) A novel method of simulating oxygen mass transfer in two-phase partitioning bioreactors. Biotechnol Bioeng 83:735–742

    Article  CAS  PubMed  Google Scholar 

  • Nielsen DR, Daugulis AJ, McLellan PJ (2005) Transient performance of a two-phase partitioning bioscrubber treating a benzene contaminated gas streams. Environ Sci Technol 39:8971–8977

    Article  CAS  PubMed  Google Scholar 

  • Nielsen DR, Sask KN, McLellan PJ, Daugulis AJ (2006) Benzene vapor treatment using a two phase partitioning bioscrubber: an improved steady-state protocol to enhance long-term operation. Bioprocess Biosyst Eng 29:229–240

    Article  CAS  PubMed  Google Scholar 

  • Nielsen DR, Daugulis AJ, McLellan PJ (2007a) Dynamic simulation of benzene vapor treatment by a two-phase partitioning bioscrubber Part I: Model development, parameter estimation, and parametric sensitivity. Biochem Eng J 36:239–249

    Article  CAS  Google Scholar 

  • Nielsen DR, Daugulis AJ, McLellan PJ (2007b) Dynamic simulation of benzene vapor treatment by a two-phase partitioning bioscrubber Part II: Model calibration, validation, and predictions. Biochem Eng J 36:250–261

    Article  CAS  Google Scholar 

  • Ortega-Calvo JJ, Birman I, Alexander M (1995) Effect of varying the rate of partitioning of phenanthrene in nonaqueous-phase liquids on biodegradation in soil slurries. Environ Sci Technol 29:2222–2225

    Article  CAS  PubMed  Google Scholar 

  • Peeva L, Yona SB, Merchuk JC (2004) Mass transfer coefficients of decane to emulsions in a bubble column reactor. Chem Eng Sci 56:5201–5206

    Article  Google Scholar 

  • Poppe W, Schippert E (1992) Das KCH-biosolv-ver-fahren in kombination mit einem biowascher herkomlicher art-eine verfahrensentwicklung zur abluftreinigung fur wasserlosliche und schwer wasserlosliche schadstoffe. In: Dragt AJ, van Ham J (eds) Biotechniques for air pollution abatements and odour control policies. Elsevier Science Publishers, Amsterdam, pp 71–76

    Google Scholar 

  • Prpich GP, Daugulis AJ (2004) Polymer development for enhanced delivery of phenol in a solid-liquid two-phase partitioning bioreactor. Biotechnol Prog 20:1725–1732

    Article  CAS  PubMed  Google Scholar 

  • Prpich GP, Adams RL, Daugulis AJ (2006) Ex situ bioremediation of phenol contaminated soil using polymer beads. Appl Microbiol Biotechnol 28:2027–2031

    CAS  Google Scholar 

  • Pulido-Mayoral N, Galindo E (2004) Phases dispersion and oxygen transfer in a simulated fermentation broth containing castor oil and proteins. Biotechnol Prog 20:1608–1613

    Article  CAS  PubMed  Google Scholar 

  • Quijano G, Revah S, Gutierrez-Rojas M, Flores-Cotera LB, Thalasso F (2009a) Oxygen transfer in three-phase airlift and stirred tank reactors using silicone oil as transfer vector. Process Biochem 44:619–624

    Article  CAS  Google Scholar 

  • Quijano G, Rocha-Rios J, Hernandez M, Villaverde S, Revah S, Muñoz R, Thalasso F (2009b) Determining the effect of solid and liquid vectors on the gaseous interfacial area in two-phase partitioning bioreactors. J Hazard Mater (submitted for publication)

  • Quijano G, Hernandez M, Villaverde S, Thalasso F, Muñoz R (2009c) A step-forward in the characterization and potential applications of solid and liquid oxygen transfer vectors. Appl Microbiol Biotechnol (in press)

  • Ramos JL, Duque E, Gallegos MT, Godoy P, Ramos-Gonzalez MI, Rojas A, Teran W, Segura A (2002) Mechanisms of solvent tolerance in Gram-negative bacteria. Annu Rev Microbiol 56:743–768

    Article  CAS  PubMed  Google Scholar 

  • Reardon KF, Mosteller DC, Rogers JDB (2000) Biodegradation kinetics of benzene, toluene, and phenol as single and mixed substrates for Pseudomonas putida F1. Biotechnol Bioeng 69:385–400

    Article  CAS  PubMed  Google Scholar 

  • Rehmann L, Daugulis AJ (2006) Biphenyl degradation kinetics by Burkholderia xenovorans LB400 in two-phase partitioning bioreactors. Chemosphere 63:972–979

    Article  CAS  PubMed  Google Scholar 

  • Rehmann L, Daugulis AJ (2008) Bioavailability of PCBs in biphasic bioreactors. Biochem Eng J 38:219–225

    Article  CAS  Google Scholar 

  • Rehmann L, Sun B, Daugulis AJ (2007) Polymer selection for biphenyl degradation in a solid-liquid two-phase partitioning bioreactor. Biotechnol Prog 23:814–819

    Article  CAS  PubMed  Google Scholar 

  • Rehmann L, Prpich GP, Daugulis AJ (2008) Remediation of PAH contaminated soils: application of a solid–liquid two-phase partitioning bioreactor. Chemosphere 73:798–804

    Article  CAS  PubMed  Google Scholar 

  • Rocha-Rios J, Bordel S, Hernández S, Revah S (2009) Methane degradation in two-phase partition bioreactors. Chem Eng J 152:289–292

    Article  CAS  Google Scholar 

  • Rols JL, Condoret JS, Fonade C, Goma G (1990) Mechanism of enhanced oxygen transfer in fermentation using emulsified oxygen-vectors. Biotechnol Bioeng 35:427–435

    Article  CAS  PubMed  Google Scholar 

  • Sander R (1999) Compilation of Henry’s law constants for inorganic and organic species of potential importance in environmental chemistry. http://www.mpch-mainz.mpg.de/∼sander/res/henry.html

  • Sikkema J, De Bont JAM, Poolman B (1995) Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev 59:201–222

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Song JH, Kinney KA (2005) Microbial response and elimination capaticy in biofilters subjected to high toluene loadings. Appl Microbial Biotechnol 68:554–559

    Article  CAS  Google Scholar 

  • Toft AM, Hurley MD, Wallington TJ, Andersen MPS, Nielsen OJ (2006) Atmospheric chemistry of C4F9O(CH2)3OC4F9 and CF3CFHCF2O(CH2)3OCF3CFHCF2: lifetimes, degradation products, and environmental impact. Chem Phys Lett 427:41–46

    Article  CAS  Google Scholar 

  • Tomei MC, Annesini MC, Rita S, Daugulis AJ (2008) Biodegradation of 4-nitrophenol in a two-phase sequencing batch reactor: concept demonstration, kinetics and modeling. Appl Microbiol Biotechnol 80:1105–1112

    Article  CAS  PubMed  Google Scholar 

  • Vandermeer KD, Daugulis AJ (2007) Enhanced degradation of a mixture of polycyclic aromatic hydrocarbons by a defined microbial consortium in a two-phase partitioning bioreactor. Biodegradation 18:211–221

    Article  CAS  PubMed  Google Scholar 

  • van Groenestijn JW, Lake ME (1999) Elimination of alkanes from off-gases using biotrickling filters containing two liquid phases. Environ Prog 18:151–155

    Article  Google Scholar 

  • Villemur R, Deziel E, Benachenhou A, Marcoux J, Gauthier E, Lepine F, Beaudet R, Comeau Y (2000) Two-liquid-phase slurry bioreactors to enhance the degradation of high-molecular-weight polycyclic aromatic hydrocarbons in soil. Biotechnol prog 16:966–972

    Article  CAS  PubMed  Google Scholar 

  • Woo SH, Lee MW, Park JM (2004) Biodegradation of phenanthrene in soil-slurry systems with different mass transfer regimes and soil contents. J Biotechnol 110:235–250

    Article  CAS  PubMed  Google Scholar 

  • Yeom SH, Daugulis AJ (1999) Benzene degradation in a two-phase partitioning bioreactor by Alcaligenes xylosoxidans Y234. Proc Biochem 36:765–772

    Article  Google Scholar 

  • Yeom SH, Daugulis AJ (2000) Development of a novel bioreactor system for treatment of gaseous benzene. Biotechnol Bioeng 72:156–165

    Article  Google Scholar 

  • Yeom SH, Daugulis AJ (2001a) Benzene degradation in a two-phase partitioning bioreactor by Alcaligenes xylosoxidans Y234. Proc Biochem 36:765–772

    Article  CAS  Google Scholar 

  • Yeom SH, Daugulis AJ (2001b) A two-phase partitioning bioreactor system for treating benzene-contaminated soil. Biotechnol Lett 23:467–473

    Article  CAS  Google Scholar 

  • Yeom SH, Dalm MCF, Daugulis AJ (2000) Tretament of hight concentration gaseous benzene streams using a novel bioreactor system. Biotechnol Lett 22:1747–1751

    Article  CAS  Google Scholar 

  • Zilouei H, Guieysse B, Mattiasson B (2008) Two-phase partitioning bioreactor for the biodegradation of high concentrations of pentachlorophenol using Sphingobium chlorophenolicum DSM 8671. Chemosphere 72:1788–1794

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors faithfully thank the financial support received from the Mexican Council of Science and Technology (Guillermo Quijano grant #164283). The Spanish Ministry of Education and Science (RYC-2007-01667, PPQ2006-08230, CONSOLIDER- CSD 2007-00055) and the Regional Government of Castilla y Leon (Ref. GR76) are also gratefully acknowledged. Katrina Penman is gratefully acknowledged for her grammatical corrections.

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Authors and Affiliations

  1. Departmento de Ingeniería Química y Tecnología del Medio Ambiente, Universidad de Valladolid, Paseo del Prado de la Magdalena, s/n, Valladolid, Spain

    Guillermo Quijano, María Hernandez, Raúl Muñoz & Santiago Villaverde

  2. Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios, Avanzados del IPN (Cinvestav), Apdo, Postal 14-740, Mexico, D. F. 07360, Mexico

    Guillermo Quijano & Frédéric Thalasso

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  1. Guillermo Quijano
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  2. María Hernandez
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  4. Raúl Muñoz
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Correspondence to Raúl Muñoz.

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Quijano, G., Hernandez, M., Thalasso, F. et al. Two-phase partitioning bioreactors in environmental biotechnology. Appl Microbiol Biotechnol 84, 829–846 (2009). https://doi.org/10.1007/s00253-009-2158-6

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  • DOI: https://doi.org/10.1007/s00253-009-2158-6

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