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Dextran sodium sulfate enhances secretion of recombinant human transferrin in Schizosaccharomyces pombe

  • Applied Microbial and Cell Physiology
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Abstract

The effect of medium supplementation on heterologous production of human serum transferrin (hTF) in the fission yeast Schizosaccharomyces pombe has been investigated. The productivity of recombinant hTF was low in wild-type S. pombe cells. To overcome this impediment, culture media supplements were screened for their ability to improve secretion of hTF. Casamino acids (CAA), which have been reported to increase heterologous protein productivity in Pichia pastoris, improved the secretion hTF by more than fourfold. An anion surfactant deoxycholate or polyethylene glycol also improved the secretion hTF. Interestingly, dextran sodium sulfate (DSS), a poly-anion surfactant, was found to enhance production of secreted hTF better than any other supplement tested. Addition of DSS in the presence of 2% CAA exhibited a synergistic effect on increasing hTF secretion, resulting in an increase of about sevenfold relative to conventional conditions. Cell growth was not found to be affected by the addition of DSS or CAA. DSS may act as a surfactant and may also facilitate the anchoring of liposomes, and these properties may contribute to efficient secretion or exocytosis through the plasma membrane.

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Acknowledgments

This work was partly supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and the Project for Development of a Technological Infrastructure for Industrial Bioprocesses on R&D of New Industrial Science and Technology Frontiers by the Ministry of Economy, Trade and Industry (METI) of Japan, as supported by the New Energy and Industrial Technology Development Organization (NEDO).

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Authors and Affiliations

  1. ASPEX Division, Research Center, Asahi Glass Co., Ltd., 1150 Hazawa-cho, Kanagawa-ku, Yokohama, 221-8755, Japan

    Hiroyuki Mukaiyama, Yuko Giga-Hama & Hideki Tohda

  2. Laboratory of Applied Microbiology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan

    Hiroyuki Mukaiyama & Kaoru Takegawa

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  1. Hiroyuki Mukaiyama
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  2. Yuko Giga-Hama
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  3. Hideki Tohda
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  4. Kaoru Takegawa
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Correspondence to Kaoru Takegawa.

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Mukaiyama, H., Giga-Hama, Y., Tohda, H. et al. Dextran sodium sulfate enhances secretion of recombinant human transferrin in Schizosaccharomyces pombe . Appl Microbiol Biotechnol 85, 155–164 (2009). https://doi.org/10.1007/s00253-009-2130-5

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