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Comparison of engineered Saccharomyces cerevisiae and engineered Escherichia coli for the production of an optically pure keto alcohol

  • Biotechnological Products and Process Engineering
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Abstract

In this study, the production of enantiomerically pure (1R,4S,6S)-6-hydroxy-bicyclo[2.2.2]octane-2-one ((−)-2) through stereoselective bioreduction was used as a model reaction for the comparison of engineered Saccharomyces cerevisiae and engineered Escherichia coli as biocatalysts. For both microorganisms, over-expression of the gene encoding the NADPH-dependent aldo-keto reductase YPR1 resulted in high purity of the keto alcohol (−)-2 (>99% ee, 97–98% de). E. coli had three times higher initial reduction rate but S. cerevisiae continued the reduction reaction for a longer time period, thus reaching a higher conversion of the substrate (95%). S. cerevisiae was also more robust than E. coli, as demonstrated by higher viability during bioreduction. It was also investigated whether the NADPH regeneration rate was sufficient to supply the over-expressed reductase with NADPH. Five strains of each microorganism with varied carbon flux through the NADPH regenerating pentose phosphate pathway were genetically constructed and compared. S. cerevisiae required an increased NADPH regeneration rate to supply YPR1 with co-enzyme while the native NADPH regeneration rate was sufficient for E. coli.

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Acknowledgements

This work was financed by the Research School in Pharmaceutical Sciences (FLÄK) and the Royal Physiographic Society in Lund and the Swedish research council (VR). We thank Professor Bärbel Hahn-Hägerdal for valuable discussions and Mathieu Heulot for his help with the E. coli-catalysed reductions.

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Authors and Affiliations

  1. Department of Applied Microbiology, Center for Chemistry and Chemical engineering, Lund University, P.O. Box 124, 221 00, Lund, Sweden

    Nádia Skorupa Parachin, Magnus Carlquist & Marie F. Gorwa-Grauslund

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  1. Nádia Skorupa Parachin
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  2. Magnus Carlquist
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  3. Marie F. Gorwa-Grauslund
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Correspondence to Marie F. Gorwa-Grauslund.

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Nádia Skorupa Parachin and Magnus Carlquist have contributed equally to the paper.

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Skorupa Parachin, N., Carlquist, M. & Gorwa-Grauslund, M.F. Comparison of engineered Saccharomyces cerevisiae and engineered Escherichia coli for the production of an optically pure keto alcohol. Appl Microbiol Biotechnol 84, 487–497 (2009). https://doi.org/10.1007/s00253-009-1964-1

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  • DOI: https://doi.org/10.1007/s00253-009-1964-1

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