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Transcriptional profiles of response to terbinafine in Trichophyton rubrum

  • Genomics and Proteomics
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Abstract

Trichophyton rubrum is a good model for the study of human pathogenic filamentous fungi. The antifungal agent terbinafine (TRB) shows specific activity against T. rubrum. To identify the transcriptional profiles of response to TRB in T. rubrum, a cDNA microarray was constructed from the expressed sequence tags of different phase cDNA libraries and transcriptional profiles of the response to TRB were determined. Exposure to TRB decreased the transcription of some genes involved in the ergosterol biosynthesis pathway, including ERG2, ERG4, ERG24, and ERG25, and induced the expression of some genes involved in lipid metabolism such as ERG10, ERG13, and INO1. TRB affected transcription of multidrug-resistance genes and some genes encoding ribosomal proteins.

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Acknowledgments

We are grateful to Ruoyu Li (Research Center for Medical Mycology, Peking University) for providing strain T. rubrum BMU 01672 and for helpful discussions. Financial supports for this work came from the National High Technology Research and Development Program of China (accession number 2006AA020504) and National Key Technologies R&D Programme (accession number 2002BA711A14).

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Authors and Affiliations

  1. State Key Laboratory for Molecular Virology and Genetic Engineering, Beijing, 100176, China

    Wenliang Zhang, Jian Yang, Lingling Wang, Junping Peng & Qi Jin

  2. Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing, 100730, China

    Qi Jin

  3. Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China

    Lu Yu

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  1. Wenliang Zhang
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  2. Lu Yu
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  4. Lingling Wang
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Correspondence to Qi Jin.

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Zhang, W., Yu, L., Yang, J. et al. Transcriptional profiles of response to terbinafine in Trichophyton rubrum . Appl Microbiol Biotechnol 82, 1123–1130 (2009). https://doi.org/10.1007/s00253-009-1908-9

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  • DOI: https://doi.org/10.1007/s00253-009-1908-9

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