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Interruption of glycerol pathway in industrial alcoholic yeasts to improve the ethanol production

  • Applied Genetics and Molecular Biotechnology
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Abstract

The two homologous genes GPD1 and GPD2, encoding two isoenzymes of NAD+-dependent glycerol-3-phosphate dehydrogenase in industrial yeast Saccharomyces cerevisiae CICIMY0086, had been deleted. The obtained two kinds of mutants gpd1Δ and gpd2Δ were studied under alcoholic fermentation conditions. gpd1Δ mutants exhibited a 4.29% (relative to the amount of substrate consumed) decrease in glycerol production and 6.83% (relative to the amount of substrate consumed) increased ethanol yield while gpd2Δ mutants exhibited a 7.95% (relative to the amount of substrate consumed) decrease in glycerol production and 7.41% (relative to the amount of substrate consumed) increased ethanol yield compared with the parental strain. The growth rate of the two mutants were slightly lower than that of the wild type under the exponential phase whereas ANG1 (gpd1Δ) and the decrease in glycerol production was not accompanied by any decline in the protein content of the strain ANG1 (gpd1Δ) but a slight decrease in the strain ANG2 (gpd2Δ). Meanwhile, dramatic decrease of acetate acid formation was observed in strain ANG1 (gpd1Δ) and ANG2 (gpd2Δ) compared to the parental strain. Therefore, it is possible to improve the ethanol yield by interruption of glycerol pathway in industrial alcoholic yeast.

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References

  • Albers E, Larsson C, Lidén G, Niklasson C, Gustafsson L (1996) Influence of the nitrogen source on Saccharomyces cerevisiae anaerobic growth and product formation. Appl Environ Microbiol 62:3187–3195

    Article  CAS  Google Scholar 

  • André L, Hemming A, Adler L (1991) Osmoregulation in Saccharomyces cerevisiae. Studies on the osmotic induction of glycerol production and glycerol 3-phosphate dehydrogenase (NAD+). FEBS Lett 286:13–17

    Article  Google Scholar 

  • Ansell R, Granath K, Hohmann S, Thevelein JM, Adler L (1997) The two isoenzymes for yeast NAD+-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in c and redox regulation. EMBO J 16:2179–2187

    Article  CAS  Google Scholar 

  • Björkqvist S, Ansell R, Adler L, Lidén G (1997) Physiological response to anaerobicity of glycerol-3-phosphate dehydrogenase mutants of Saccharomyces cerevisiae. Appl Environ Microbiol 63:128–132

    Article  Google Scholar 

  • De Vries S, van Witzenburg R, Grivell LA, Marres CAM (1992) Primary structure and import pathway of the rotenone-insensitive NADH-ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae. Eur J Biochem 203:587–592

    Article  Google Scholar 

  • Eriksson P, André L, Ansell R, Blomberg A, Adler L (1995) Cloning and characterisation of GPD2, a second gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD+) in Saccharomyces cerevisiae, and its comparison with GPD1. Mol Microbiol 17:95–107

    Article  CAS  Google Scholar 

  • Larsson K, Ansell R, Eriksson P, Adler L (1993) A gene encoding sn-glycerol-3-phosphate dehydrogenase (NAD+) complements an osmosensitive mutant of Saccharomyces cerevisiae. Mol Microbiol 10:1101–1111

    Article  CAS  Google Scholar 

  • Nissen TL, Schulze U, Nielsen J, Villadsen J (1997) Flux distributions in anaerobic, glucose-limited continuous cultivations of Saccharomyces cerevisiae. Microbiology 143:203–218

    Article  CAS  Google Scholar 

  • Nissen TL, Hamann CW, Kielland-Brandt MC, Nielsen J, Villadsen J (2000) Anaerobic and aerobic batch cultivations of Saccharomyces cerevisiae mutants impaired in glycerol synthesis. Yeast 16:463–474

    Article  CAS  Google Scholar 

  • Remize F, Barnavon L, Dequin S (2001) Glycerol export and glycerol-3-phosphate dehydrogenase, but not glycerol phosphatase, are rate limiting for glycerol production in Saccharomyces cerevisiae. Metab Eng 3:301–312

    Article  CAS  Google Scholar 

  • Valadi H, Larsson C, Gustafsson L (1998) Improved ethanol production by glycerol-3-phosphate dehydrogenase mutants of Saccharomyces cerevisiae. Appl Microbiol Biotechnol 4:434–439

    Article  Google Scholar 

  • Verduyn C, Stouthamer AH, Scheffers WA, van Dijken JP (1991) A theoretical evaluation of growth yields of yeasts. Antonie Van Leeuwenhoek 59:49–63

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was financial supported by the Natural Science Foundation of China (20706024), the “863” Program(2006AA020101, 2007AA10Z359), the Open Project Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China (KLIB-KF200701) and Technology Development Programme for institute of Yunnan Province (No.2006KFZX-10).

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Zhong-peng Guo, Liang Zhang, Zhong-yang Ding, Zheng-Xiang Wang & Gui-Yang Shi

  2. Laboratory of Biomass Refinery and Processing, School of Biotechnology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Zhong-peng Guo, Liang Zhang, Zhong-yang Ding & Gui-Yang Shi

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  1. Zhong-peng Guo
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  2. Liang Zhang
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  3. Zhong-yang Ding
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Correspondence to Gui-Yang Shi.

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Guo, Zp., Zhang, L., Ding, Zy. et al. Interruption of glycerol pathway in industrial alcoholic yeasts to improve the ethanol production. Appl Microbiol Biotechnol 82, 287–292 (2009). https://doi.org/10.1007/s00253-008-1777-7

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  • DOI: https://doi.org/10.1007/s00253-008-1777-7

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