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Inhibition of degradation and aggregation of recombinant human consensus interferon-α mutant expressed in Pichia pastoris with complex medium in bioreactor

  • Applied Microbial and Cell Physiology
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Abstract

The methylotrophic yeast Pichia pastoris has been used for the expression of many proteins. However, limitations such as protein degradation and aggregation became obvious when secreting heterologous protein-recombinant human consensus interferon-α mutant. Here, we investigate the effect of induction temperature on the yield and stability of interferon mutant expressed by P. patoris with buffered complex medium. The best results in terms of interferon mutant bioactivity and specific bioactivity were obtained when the microorganism was induced at 15°C, which were 2.91 × 108 ± 0.3 × 108 and 2.26 × 108 ± 0.23 × 108 IU mg−1, respectively. At the same time, the cells grew fast owing to high AOX1-specific activity, and interferon mutant expression level reached 1.23 g l−1, which was almost 30 times higher than that in the flask. Also, the proteolytic degradation of interferon mutant was inhibited completely because of lower protease bioactivity probably due to a reduced cell death rate at lower temperatures as well as protection of yeast extract and peptone in complex medium. In addition, interferon mutant aggregation was repressed significantly by the addition of Tween-80, and a specific bioactivity of 7.35 × 108 ± 0.56 × 108 IU mg−1 was obtained. These results should be applicable to other low-stability recombinant proteins expressed in P. pastoris.

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Acknowledgment

This work was supported by China National 973 Plan (no. 2007CB714306).

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, National Engineering Center of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People’s Republic of China

    Dan Wu, Yu-You Hao, Ju Chu, Ying-Ping Zhuang & Si-Liang Zhang

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  1. Dan Wu
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  2. Yu-You Hao
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  3. Ju Chu
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  4. Ying-Ping Zhuang
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  5. Si-Liang Zhang
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Correspondence to Si-Liang Zhang.

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Wu, D., Hao, YY., Chu, J. et al. Inhibition of degradation and aggregation of recombinant human consensus interferon-α mutant expressed in Pichia pastoris with complex medium in bioreactor. Appl Microbiol Biotechnol 80, 1063–1071 (2008). https://doi.org/10.1007/s00253-008-1629-5

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  • DOI: https://doi.org/10.1007/s00253-008-1629-5

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