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Efficient production of active form of recombinant cassava hydroxynitrile lyase using Escherichia coli in low-temperature culture

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An Erratum to this article was published on 01 September 2008

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Abstract

Overexpression and production of the high concentration of hydroxynitrile lyase from cassava (Manihot esculenta (MeHNL, EC 4.1.2.39)) were investigated. Hydroxynitrile lyase is a useful enzyme for the production of optically active cyanohydrin compounds. The production of MeHNL was increased by changing the rare codons of the original sequence of cassava MeHNL. However, most of the produced MeHNL was in the insoluble form. In order to increase the solubility of MeHNL, the effects of the cultivation temperature were investigated. When the cultivation temperature was reduced, the cell yield and the ratio of soluble MeHNL increased significantly. The enzyme activity and yield at low-temperature cultures (17 °C) were 850 times higher than those obtained at the optimum growth temperature of 37 °C. The rate of MeHNL production in the present study was calculated as 3,000 unit/h. Low-temperature cultivation was very effective in improving the productivity of the active form of MeHNL. Unlike the temperature-shift method, low-temperature cultivation has more potential for the large-scale production of MeHNL for the optically active cyanohydrin production.

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  • 01 September 2008

    The original version of this article unfortunately contained a mistake. The positions of the asterisks of Fig. 1 were shifted. The correct version is given here.

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Authors and Affiliations

  1. Strategic Technology Research Center, Nippon Shokubai Co., Ltd, 1-25-12, Kannondai, Tsukuba, Ibaraki, 305-0856, Japan

    Hisashi Semba & Eita Ichige

  2. Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, 606-8501, Japan

    Tadayuki Imanaka & Haruyuki Atomi

  3. Graduate School of Life and Environmental Sciences, Tsukuba University, Tsukuba, Ibaraki, 305-8572, Japan

    Hisashi Semba & Hideki Aoyagi

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  1. Hisashi Semba
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  2. Eita Ichige
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  3. Tadayuki Imanaka
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  4. Haruyuki Atomi
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  5. Hideki Aoyagi
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Correspondence to Hisashi Semba.

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Semba, H., Ichige, E., Imanaka, T. et al. Efficient production of active form of recombinant cassava hydroxynitrile lyase using Escherichia coli in low-temperature culture. Appl Microbiol Biotechnol 79, 563–569 (2008). https://doi.org/10.1007/s00253-008-1464-8

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  • DOI: https://doi.org/10.1007/s00253-008-1464-8

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