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Effect of electrical stimulation on human immunodeficiency virus type-1 infectivity

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Abstract

We examined the effects of electrical stimulation on HIV-1-adsorbed MAGIC-5 (MAGIC-5/HIV-1) cells and unadsorbed MAGIC-5 (MAGIC-5) cells. When MAGIC-5 cells were stimulated by a constant d.c. potential of 1.0 V (vs Ag/Agcl) immediately after HIV-1LAI infection, infectivity was more affected by electrical stimulation than by cell membrane damage. In particular, after application of potential at 1.0 V for 5 min, about 1% of the membranes of the MAGIC-5/HIV-1LAI cells were damaged, but the infectivities of both HIV-1LAI and HIV-1NL43-luc cells decreased about 37 and 44%, respectively (p < 0.05). After application of potential at 1.0 V for 5 min, the mean fluorescence intensities (MFIs) of highly reactive oxygen species (hROS) and nitric oxide (NO) in MAGIC-5/HIV-1NL43-Luc cells were significantly increased compared with that of unstimulated MAGIC-5/HIV-1NL43-Luc cells (p < 0.01). However, the MFIs of hROS and NO in MAGIC-5 cells were also increased, to the same level, by electrical stimulation for 5 min. These results suggest that HIV-1 adsorbed onto or invading cells is damaged by direct or indirect effects of electrical stimulation, resulting in a decrease in HIV-1 infectivity. It is also suggested that hROS and NO induced by electrical stimulation are important factors for inhibiting HIV-1 infection.

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Acknowledgment

We thank Dr. Y. Maeda and Dr. H. Hoshino for providing HIV NL-Luc plasmid and CXN-FLenv plasmid, respectively. We also thank Dr. M. Tatsumi for supplying MAGIC-5 cells. We thank T. Nakamura and S. Yamamoto for technical assistance.

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Authors and Affiliations

  1. Department of Health Science, Kumamoto University School of Medicine, 4-24-1 Kuhonji, Kumamoto, 862-0976, Japan

    Etsuko Kumagai

  2. Graduate School of Science and Technology, Kumamoto University, 2-39-1, Kumamoto, 860-8555, Japan

    Masato Tominaga & Shouichiro Nagaishi

  3. Department of Medical Virology, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto, 860-8556, Japan

    Shinji Harada

Authors
  1. Etsuko Kumagai
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  2. Masato Tominaga
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  3. Shouichiro Nagaishi
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  4. Shinji Harada
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Correspondence to Etsuko Kumagai.

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Kumagai, E., Tominaga, M., Nagaishi, S. et al. Effect of electrical stimulation on human immunodeficiency virus type-1 infectivity. Appl Microbiol Biotechnol 77, 947–953 (2007). https://doi.org/10.1007/s00253-007-1214-3

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  • DOI: https://doi.org/10.1007/s00253-007-1214-3

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