Abstract
To increase the yield of heterologous production of the class II bacteriocin DvnRV41 with Escherichia coli Origami (DE3) (pLysS/pCR03), induction of bacteriocin gene expression was optimized by varying the inducer isopropyl β-d-thiogalactopyranoside (IPTG) concentration (0–2 mM), and controlled batch and fed-batch cultures were tested on a 2-L scale. A concentration of 0.5 mM IPTG was found to be optimal for cell growth and bacteriocin production. Shake flask cultivation of E. coli Origami (DE3) (pLysS/pCR03) gave biomass and bacteriocin yields of 1.54 ± 0.06 g cdw/l and 18 ± 1 mg DvnRV41/l, respectively. Biomass (2.70 ± 0.06 and 6.8 ± 0.6 g cdw/l, respectively) and bacteriocin yields (30 and 74 mg DvnRV41 per liter, respectively) were both increased with batch and fed-batch compared to shake flask cultures. Bacteriocin yields reported in this study are among the highest published for other heterologous expression systems in shake flasks.
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Acknowledgements
Ségolène Calvez was recipient of a Ph.D. fellowship from the Région des Pays de la Loire (France). This work was supported by ETH Zurich and the VANAM II research grant (Région des Pays de la Loire), and partially by the 6th PCRD SEAFOODplus European Union integrated project.
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Yildirim, S., Konrad, D., Calvez, S. et al. Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures. Appl Microbiol Biotechnol 77, 525–531 (2007). https://doi.org/10.1007/s00253-007-1188-1
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DOI: https://doi.org/10.1007/s00253-007-1188-1