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Production of ascorbic acid glucoside by alginate-entrapped mycelia of Aspergillus niger

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Abstract

The mycelia of Aspergillus niger, cultivated in a medium containing 45 g l−1 maltose, 66 g l−1 yeast extract, and 5 g l−1 K2HPO4 at 30°C and 200 rpm, were used as a biocatalyst in the glucosylation of ascorbic acid. Free mycelia from 3-day-old culture, when used in a 6-h reaction with maltose as the acyl donor, gave 16.07 g l−1 ascorbic acid glucoside corresponding to a volumetric productivity of 2.68 g l−1 h−1 and a conversion of 67%. Mycelia from 3-day-old cultures were entrapped in calcium alginate beads and used as a catalyst in the glucosylation of ascorbic acid. An ascorbic acid-to-maltose molar ratio of 1:9 was found to be optimum, and the conversion reached 75% after 12 h. The concentration of ascorbic acid glucoside produced at this molar ratio was 17.95 g l−1, and the productivity was 1.5 g l−1 h−1. The biocatalyst was repeatedly used in a fixed bed bioreactor for the synthesis of ascorbic acid glucoside and approximately 17 g l−1 of ascorbic acid glucoside corresponding to a volumetric productivity of 1.42 g l−1 h−1 was produced in each use. The conversion was retained at 70% in each use. The entrapped mycelia also exhibited exceptionally high reusability and storage stability. The product was purified to 85% by anion exchange and gel permeation chromatography with a final yield of 75%.

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Acknowledgment

This research was supported by grants (NSC 94-2214-E-006-016) from the National Science Council of Taiwan.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan

    Hsin-Ju Hsieh, Kai-Yu Tung & I-Ming Chu

  2. Department of Engineering, The University of Waikato, Hamilton, New Zealand

    Giridhar R. Nair

  3. Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan

    Wen-Teng Wu

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  1. Hsin-Ju Hsieh
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  2. Kai-Yu Tung
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  3. Giridhar R. Nair
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  4. I-Ming Chu
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  5. Wen-Teng Wu
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Correspondence to Wen-Teng Wu.

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Hsieh, HJ., Tung, KY., Nair, G.R. et al. Production of ascorbic acid glucoside by alginate-entrapped mycelia of Aspergillus niger . Appl Microbiol Biotechnol 77, 53–60 (2007). https://doi.org/10.1007/s00253-007-1148-9

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  • DOI: https://doi.org/10.1007/s00253-007-1148-9

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