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Enhanced iturin A production by Bacillus subtilis and its effect on suppression of the plant pathogen Rhizoctonia solani

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Abstract

Enhanced production of the antibiotic iturin A by Bacillus subtilis RB14-CS reached 4.4 g L−1 in SM medium containing soybean meal and maltose, which was 16-fold and 2.2-fold higher than that in original and modified number 3S media, respectively. When various volumes of RB14-CS cultures grown in SM medium were applied to pot tests of tomato damping-off caused by Rhizoctonia solani, damping-off was dose-dependently suppressed by the cultures. Suppression by SM-grown cultures was significantly more effective than that by cultures grown in original or modified number 3S media. The iturin A concentrations in soil decreased to undetectable levels after 17 days of cultivation in pot tests, indicating that iturin A has a low persistence in soil.

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Authors and Affiliations

  1. Chemical Resources Laboratory, Tokyo Institute of Technology, R1-29-4259 Nagatsuta, Midori-ku, Yokohama, 226-8503, Japan

    S. Mizumoto, M. Hirai & M. Shoda

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  1. S. Mizumoto
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  2. M. Hirai
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  3. M. Shoda
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Correspondence to M. Shoda.

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Mizumoto, S., Hirai, M. & Shoda, M. Enhanced iturin A production by Bacillus subtilis and its effect on suppression of the plant pathogen Rhizoctonia solani . Appl Microbiol Biotechnol 75, 1267–1274 (2007). https://doi.org/10.1007/s00253-007-0973-1

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  • DOI: https://doi.org/10.1007/s00253-007-0973-1

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