Abstract
The Cr(VI)-reducing bacterial strain MCMB-821 was isolated from the alkaline crater lake of Lonar and was identified as Burkholderia cepacia. MCMB-821 was resistant to 1,000-ppm Cr(VI) and reduced 98% of the 75 ppm Cr(VI) within 36 h at pH 9.0 in the presence of 2% salt and lactose as the electron donor. The chromate-reducing efficiency of MCMB-821 was comparable under both aerobic as well as anaerobic conditions. Electron paramagnetic resonance spectroscopy data suggested that MCMB-821 reduced Cr(VI) to Cr(III) via the formation of transient Cr(V) intermediate. The chromate-reducing ability of MCMB-821 was suppressed in the presence of membrane inhibitors and enhanced in the presence of 2,4-dinitrophenol, suggesting the involvement of electron transport chain in the Cr(VI) bioreduction.
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Acknowledgment
The authors thank Dr. Srinivas Darbha and L. Satyanarayana, National Chemical Laboratories, Pune, 411008, India, for the EPR analysis. The financial support provided by the University of Pune is acknowledged.
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Table S1
Characteristics of strain MCMB-821 (DOC 50 kb)
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Wani, R., Kodam, K.M., Gawai, K.R. et al. Chromate reduction by Burkholderia cepacia MCMB-821,isolated from the pristine habitat of alkaline crater lake. Appl Microbiol Biotechnol 75, 627–632 (2007). https://doi.org/10.1007/s00253-007-0862-7
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DOI: https://doi.org/10.1007/s00253-007-0862-7