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Production of tyrosine from sucrose or glucose achieved by rapid genetic changes to phenylalanine-producing Escherichia coli strains

  • Applied Genetics and Molecular Biotechnology
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Abstract

Escherichia coli K12 strains producing l-phenylalanine were converted to l-tyrosine-producing strains using a novel genetic method for gene replacement. We deleted a region of the E. coli K12 chromosome including the pheA gene encoding chorismate mutase/prephenate dehydratase, its leader peptide (pheL), and its promoter using a new polymerase chain reaction-based method that does not leave a chromosomal scar. For high level expression of tyrA, encoding chorismate mutase/prephenate dehydrogenase, its native promoter was replaced with the strong trc promoter. The linked ΔpheLA and Ptrc-tyrA::KanR genetic modifications were moved into l-phenylalanine producing strains by generalized transduction to convert l-phenylalanine-producing strains to l-tyrosine-producing strains. Moreover, introduction of a plasmid carrying genes responsible for sucrose degradation into these strains enabled l-tyrosine-production from sucrose.

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Acknowledgment

We thank B. Wanner for provision of plasmids pKD4 and pKD46.

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Authors and Affiliations

  1. Biochemical Sciences and Engineering, DuPont Central Research and Development, Experimental Station, Wilmington, DE, 19880, USA

    Monica M. Olson, Lori J. Templeton, Wonchul Suh, F. Sima Sariaslani, Anthony A. Gatenby & Tina K. Van Dyk

  2. Department of Biology, Texas A&M University, College Station, TX, 83843-3052, USA

    Philip Youderian

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  1. Monica M. Olson
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  2. Lori J. Templeton
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  3. Wonchul Suh
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  4. Philip Youderian
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  5. F. Sima Sariaslani
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  7. Tina K. Van Dyk
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Correspondence to Tina K. Van Dyk.

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Olson, M.M., Templeton, L.J., Suh, W. et al. Production of tyrosine from sucrose or glucose achieved by rapid genetic changes to phenylalanine-producing Escherichia coli strains. Appl Microbiol Biotechnol 74, 1031–1040 (2007). https://doi.org/10.1007/s00253-006-0746-2

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  • DOI: https://doi.org/10.1007/s00253-006-0746-2

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