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A simple method for extracting C-phycocyanin from Spirulina platensis using Klebsiella pneumoniae

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Abstract

C-phycocyanin (C-PC) was extracted from fresh Spirulina platensis by deploying a species of non-pathogenic nitrogen-fixing bacteria, namely, Klebsiella pneumoniae. The algal slurry was neither washed nor centrifuged; the bacterial culture was poured into the slurry, the vessel sealed, and crude C-PC extracted after about 24 h. The extraction was clean and efficient, and the purity and concentration of C-PC proved to be of adequate quality.

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Acknowledgements

The authors are touched by the patience and care shown by three unknown reviewers; the manuscript has benefited from their precision and good advice. Thanks are also due to our friends, Mr. Li B.X., Teng L.J. and Mrs. Qin X. Ch., for the discussions on this study. This work was supported by the National Natural Science Foundation of China (NSFC) grant (No. 40473051) and Resource, Environment and GIS Key laboratory of Beijing City.

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Authors and Affiliations

  1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Photosynthesis Research Center, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

    Y. Zhu, X. B. Chen, K. B. Wang, Y. X. Li, K. Z. Bai & T. Y. Kuang

  2. Resource, Environment and GIS Key Laboratory of Beijing City, Capital Normal University, Beijing, 100037, China

    H. B. Ji

  3. Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China

    Y. Zhu

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  1. Y. Zhu
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  2. X. B. Chen
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  3. K. B. Wang
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  4. Y. X. Li
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  5. K. Z. Bai
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  6. T. Y. Kuang
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  7. H. B. Ji
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Correspondence to T. Y. Kuang or H. B. Ji.

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Zhu, Y., Chen, X.B., Wang, K.B. et al. A simple method for extracting C-phycocyanin from Spirulina platensis using Klebsiella pneumoniae . Appl Microbiol Biotechnol 74, 244–248 (2007). https://doi.org/10.1007/s00253-006-0636-7

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  • DOI: https://doi.org/10.1007/s00253-006-0636-7

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