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A chemostat study of Streptomyces peucetius var. caesius N47

  • Biotechnological Products and Process Engineering
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Abstract

The behavior of Streptomyces peucetius var. caesius N47 was studied in a glucose limited chemostat with a complex cultivation medium. The steady-state study yielded the characteristic constants μ max over 0.10 h−1, Y XS 0.536 g g−1, and mS 0.54 mg g−1 h−1. The product of secondary metabolism, ɛ-rhodomycinone, was produced with characteristics Y PX 12.99 mg g−1 and m P 1.20 mg g−1 h−1. Significant correlations were found for phosphate and glucose consumption with biomass and ɛ-rhodomycinone production. Metabolic flux analysis was conducted to estimate intracellular fluxes at different dilution rates. TCA, PPP, and shikimate pathway fluxes exhibited bigger values with production than with growth. Environmental perturbation experiments with temperature, airflow, and pH changes on a steady-state chemostat implied that an elevation of pH could be the most effective way to shift the cells from growing to producing, as the pH change induced the biggest transient increase to the calculated ɛ-rhodomycinone flux.

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Acknowledgements

The authors wish to thank Finnish Funding Agency for Technology and Innovation (TEKES) for funding, and Marjaana Rytelä and Auli Murrola for the HPLC analysis.

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Authors and Affiliations

  1. Laboratory of Bioprocess Engineering, Helsinki University of Technology, Helsinki, P.O. Box 6100, 02015 HUT, Finland

    Kristiina Kiviharju, Ulla Moilanen, Matti Leisola & Tero Eerikäinen

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  1. Kristiina Kiviharju
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  2. Ulla Moilanen
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  3. Matti Leisola
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  4. Tero Eerikäinen
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Correspondence to Kristiina Kiviharju.

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Kiviharju, K., Moilanen, U., Leisola, M. et al. A chemostat study of Streptomyces peucetius var. caesius N47. Appl Microbiol Biotechnol 73, 1267–1274 (2007). https://doi.org/10.1007/s00253-006-0607-z

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  • DOI: https://doi.org/10.1007/s00253-006-0607-z

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