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Targeted deletion of the uvrBA operon and biological containment in the industrially important Bacillus licheniformis

  • Applied Genetics and Molecular Biotechnology
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Abstract

From a Bacillus licheniformis wild type as well as a defined asporogenous derivative, stable UV hypersensitive mutants were generated by targeted deletion of the uvrBA operon, encoding highly conserved key components of the nucleotide excision repair. Comparative studies, which included the respective parental strains, revealed no negative side effects of the deletion, neither on enzyme secretion nor on vegetative propagation. Thus, the uvrBA locus proved to be a useful deletion target for achieving biological containment in this industrially exploited bacterium. In contrast to recA mutants, which also display UV hypersensitivity, further strain development via homologous recombination techniques will be still possible in such uvr mutants.

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Acknowledgement

This work was supported by the Federal Ministry of Education and Research (BMBF, Bonn-Bad Godesberg, Germany), grant no. 0313645.

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Authors and Affiliations

  1. Westfälische Wilhelms-Universität Münster, Institut für Molekulare Mikrobiologie und Biotechnologie, Corrensstrasse 3, 48149, Münster, Germany

    Jens Waldeck, Heike Meyer-Rammes, Hannes Nahrstedt & Friedhelm Meinhardt

  2. Henkel KGaA, VTB-Enzymtechnologie, Henkelstrasse 67, 40191, Düsseldorf, Germany

    Renée Eichstädt & Susanne Wieland

Authors
  1. Jens Waldeck
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  2. Heike Meyer-Rammes
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  3. Hannes Nahrstedt
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  4. Renée Eichstädt
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  5. Susanne Wieland
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  6. Friedhelm Meinhardt
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Correspondence to Friedhelm Meinhardt.

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Waldeck, J., Meyer-Rammes, H., Nahrstedt, H. et al. Targeted deletion of the uvrBA operon and biological containment in the industrially important Bacillus licheniformis . Appl Microbiol Biotechnol 73, 1340–1347 (2007). https://doi.org/10.1007/s00253-006-0602-4

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  • DOI: https://doi.org/10.1007/s00253-006-0602-4

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