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Effect of flocculation on performance of arming yeast in direct ethanol fermentation

  • Biotechnological Products and Process Engineering
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Abstract

In the direct ethanol fermentation of raw starch by arming yeast with α-amylase and glucoamylase, it is preferable to use a flocculent yeast because it can be recovered without centrifugation. Three types of arming yeast system, I (nonflocculent), II (mildly flocculent), and III (heavily flocculent), were constructed and their fermentation performances were compared. With an increase in the degree of flocculation, specific ethanol production rate for soluble starch decreased (0.19, 0.17, and 0.12 g g-dry-cell−1 h−1 for systems I, II, and III, respectively), but that for raw starch did not decrease as much as expected (0.06, 0.06, and 0.04 g g-dry-cell−1 h−1 for systems I, II and III, respectively). Microscopic observation revealed that many starch granules were captured in the yeast flocs in system III during the direct ethanol fermentation of raw starch. It was suggested that the capture of starch granules increases apparent substrate concentration for amylolytic enzymes in arming yeast cell flocs; thus, the specific ethanol production rate of system III was kept at a level comparable to those of the other systems.

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Authors and Affiliations

  1. Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Khaw Teik Seong, Yoshio Katakura, Kazuaki Ninomiya & Suteaki Shioya

  2. Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, 657-8501, Japan

    Yohei Bito, Satoshi Katahira & Akihiko Kondo

  3. Department of Applied Biochemistry, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan

    Mitsuyoshi Ueda

Authors
  1. Khaw Teik Seong
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  2. Yoshio Katakura
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  3. Kazuaki Ninomiya
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  4. Yohei Bito
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  5. Satoshi Katahira
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  6. Akihiko Kondo
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  7. Mitsuyoshi Ueda
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  8. Suteaki Shioya
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Correspondence to Suteaki Shioya.

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Seong, K., Katakura, Y., Ninomiya, K. et al. Effect of flocculation on performance of arming yeast in direct ethanol fermentation. Appl Microbiol Biotechnol 73, 60–66 (2006). https://doi.org/10.1007/s00253-006-0454-y

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  • DOI: https://doi.org/10.1007/s00253-006-0454-y

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