Abstract
The production of mannitol by Lactobacillus intermedius NNRL B-3693 using molasses as an inexpensive carbon source was evaluated. The bacterium produced mannitol (104 g/l) from molasses and fructose syrups (1:1; total sugars, 150 g/l; fructose:glucose 4:1) in 16 h. Several kinds of inexpensive organic and inorganic nitrogen sources and corn steep liquor were evaluated for their potential to replace more expensive nitrogen sources derived from Bacto-peptone and yeast extract. Soy peptone D (5 g/l) and corn steep liquor (50 g/l) were found to be suitable substitutes for Bacto-peptone (5 g/l) and Bacto-yeast extract (5 g/l), respectively. The bacterium produced 105 g mannitol per liter from the molasses and fructose syrup (1:1, total sugars 150 g/l; fructose:glucose 4:1) in 22 h using a combination of soy peptone D (5 g/l) and corn steep liquor (50 g/l). This is the first report on the production of mannitol by fermentation using molasses and corn steep liquor.
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References
De Man JC, Rogosa M, Sharpe ME (1960) A medium for the cultivation of Lactobacilli. J Appl Bacteriol 23:130–135
Johnson JC (1976) Sugar alcohols and derivatives. In: Specialized sugars for the food industry. Noyes Data, New Jersey, p 313
Korakli M, Schwarz E, Wolf G, Hammes WP (2000) Production of mannitol by Lactobacillus sanfranciscensis. Adv Food Sci 22:1–4
Makkee M, Kieboom APG, Van Bekkum H (1985) Production methods of d-mannitol. Starch/Stärke 37:136–141
Richter H, Hamann I, Unden G (2003) Use of the mannitol pathway in fructose fermentation of Oenococcus oeni due to limiting redox regeneration capacity of the ethanol pathway. Arch Microbiol 179:227–233
Saha BC (2003) Production of mannitol by fermentation. In: Saha BC (ed) Fermentation biotechnology. American Chemical Society, Washington, District of Columbia, pp 67–85
Saha BC (2004) Purification and characterization of a novel mannitol dehydrogenase from Lactobacillus intermedius. Biotechnol Prog 20:537–542
Saha BC, Nakamura LK (2003) Production of mannitol and lactic acid by fermentation with Lactobacillus intermedius NRRL B-3693. Biotechnol Bioeng 82:864–871
Smiley KL, Cadmus MC, Liepins P (1967) Biosynthesis of d-mannitol from d-glucose by Aspergillus candidus. Biotechnol Bioeng 9:365–374
Soetaert W, Buchholz K, Vandamme EJ (1995) Production of d-mannitol and d-lactic acid by fermentation with Leuconostoc mesenteroides. Agro Food Ind Hi Technol 6:41–44
Song K-H, Lee J-K, Song J-Y, Hong S-G, Baek H, Kim S-Y, Hyun H-H (2002) Production of mannitol by a novel strain of Candida magnoliae. Biotechnol Lett 24:9–12
Vandamme EJ, Soetaert W (1995) Biotechnical modification of carbohydrates. FEMS Microbiol Rev 16:163–186
von Weymarn FNW, Hujanen M, Leisola MSA (2002) Production of d-mannitol by heterofermentative lactic acid bacteria. Process Biochem 37:1207–1213
von Weymarn FNW, Kiviharju KJ, Jaaskelainen SP, Leisola MSA (2003) Scale-up of a new bacterial mannitol production process. Biotechnol Prog 19:815–821
Wisselink HW, Weusthuis RA, Eggink G, Hugenholtz J, Grobben GJ (2002) Mannitol production by lactic acid bacteria: a review. Int Dairy J 12:151–161
Yun JW, Kang SC, Song SK (1996) Microbial transformation of fructose to mannitol by Lactobacillus sp. KY-107. Biotechnol Lett 18:35–40
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The author thanks Gregory J. Kennedy for excellent technical assistance.
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Saha, B.C. A low-cost medium for mannitol production by Lactobacillus intermedius NRRL B-3693. Appl Microbiol Biotechnol 72, 676–680 (2006). https://doi.org/10.1007/s00253-006-0364-z
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DOI: https://doi.org/10.1007/s00253-006-0364-z