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A laccase from the medicinal mushroom Ganoderma lucidum

  • Biotechnologically Relevant Enzymes and Proteins
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Abstract

A protein demonstrating laccase activity and potent inhibitory activity towards human immunodeficiency virus (HIV)-1 reverse transcriptase (IC50 1.2 μM) was isolated from fresh fruiting bodies of the medicinal mushroom Ganoderma lucidum. The laccase had a novel N-terminal sequence and a molecular mass of 75 kDa, which is higher than the range (55–56 kDa) reported for most other mushroom laccases. It was isolated by sequential chromatography on DEAE-cellulose and Affi-gel blue gel and adsorption on Con A-Sepharose. Unlike some of the previously isolated laccases, it was adsorbed only on Con A-Sepharose. The enzyme required a pH of 3–5 and a temperature of 70°C to exhibit maximal activity. Minimal activity was detected at pH 6 and 7. Activity was undetectable at pH 8 and 9 and after exposure to 100°C for 10 min.

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Acknowledgement

We thank Miss Fion Yung for her skilled secretarial assistance.

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Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, 100094, China

    H. X. Wang

  2. Ministry of Agriculture, Beijing, China

    H. X. Wang

  3. Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

    T. B. Ng

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  1. H. X. Wang
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  2. T. B. Ng
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Correspondence to T. B. Ng.

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Wang, H.X., Ng, T.B. A laccase from the medicinal mushroom Ganoderma lucidum . Appl Microbiol Biotechnol 72, 508–513 (2006). https://doi.org/10.1007/s00253-006-0314-9

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  • DOI: https://doi.org/10.1007/s00253-006-0314-9

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