Skip to main content
SpringerLink
Log in

d-Mannitol formation from d-glucose in a whole-cell biotransformation with recombinant Escherichia coli

  • Biotechnological Products and Process Engineering
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Recently, we reported on the construction of a whole-cell biotransformation system in Escherichia coli for the production of d-mannitol from d-fructose (Kaup B, Bringer-Meyer S, Sahm H (2004) Metabolic engineering of Escherichia coli: construction of an efficient biocatalyst for d-mannitol formation in a whole-cell biotransformation. Appl Microbiol Biotechnol 64:333–339). Supplementation of this strain with extracellular glucose isomerase resulted in the formation of 800 mM d-mannitol from 1,000 mM d-glucose. Co-expression of the xylA gene of E. coli in the biotransformation strain resulted in a d-mannitol concentration of 420 mM from 1,000 mM d-glucose. This is the first example of conversion of d-glucose to d-mannitol with direct coupling of a glucose isomerase to the biotransformation system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Ananichev AV, Ulezlo IV, Egorov AM, Bezborodov AM, Berezin IV (1980) Inhibition of glucose isomerase from Actinomyces olivocinereus 154 by polyols. Biokemia 45:992–999

    CAS  Google Scholar 

  • Baek H, Song KH, Park SM, Kim SY, Hyun HH (2003) Role of glucose in the bioconversion of fructose into mannitol by Candida magnoliae. Biotechnol Lett 25(10):761–765

    CAS  PubMed  Google Scholar 

  • Barnell WO, Yi KC, Conway T (1990) Sequence and genetic organization of a Zymomonas mobilis gene cluster that encodes several enzymes of glucose metabolism. J Bacteriol 172(12):7227–7240

    CAS  PubMed  PubMed Central  Google Scholar 

  • Bhosale SH, Rao MB, Deshpande VV (1996) Molecular and industrial aspects of glucose isomerase. Microbiol Rev 60(2):280–300

    CAS  PubMed  PubMed Central  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein–dye binding. Anal Biochem 72:248–254

    CAS  PubMed  Google Scholar 

  • Briggs KA, Lancashire WE, Hartley BS (1984) Molecular cloning, DNA structure and expression of the Escherichia colid-xylose isomerase. EMBO J 3(3):611–616

    CAS  PubMed  PubMed Central  Google Scholar 

  • Chen WP (1980a) Glucose isomerase. Process Biochem 15:30–35

    CAS  Google Scholar 

  • Chen WP (1980b) Glucose isomerase. Process Biochem 15:36–41

    CAS  Google Scholar 

  • David JD, Wiesmeyer H (1970) Control of xylose metabolism in Escherichia coli. Biochim Biophys Acta 201(3):497–499

    CAS  PubMed  Google Scholar 

  • Ferain T, Schank AN, Delcour J (1996) 13C Nuclear magnetic resonance analysis of glucose and citrate end products in an ldhL–ldhD double-knockout strain of Lactobacillus plantarum. J Bacteriol 178:7311–7315

    CAS  PubMed  PubMed Central  Google Scholar 

  • Galkin A, Kulakova L, Tishkov V, Esaki N, Soda K (1995) Cloning of formate dehydrogenase gene from a methanol-utilizing bacterium Mycobacterium vaccae N10. Appl Microbiol Biotechnol 44(3–4):479–483

    CAS  PubMed  Google Scholar 

  • Hahn G, Kaup B, Bringer-Meyer S, Sahm H (2003) A zinc-containing mannitol-2-dehydrogenase from Leuconostoc pseudomesenteroides ATCC 12291: purification of the enzyme and cloning of the gene. Arch Microbiol 179:101–107

    CAS  PubMed  Google Scholar 

  • Haltrich D, Nidetzky B, Miemietz G, Gollhofer D, Lutz S, Stolz P, Kulbe KD (1996) Simultaneous enzymatic synthesis of mannitol and gluconic acid: I. Characterization of the enzyme system. Biocatal Biotransform 14:31–45

    CAS  Google Scholar 

  • Hanahan D (1983) Studies on the transformation of E. coli with plasmids. J Mol Biol 166:557–580

    CAS  PubMed  Google Scholar 

  • Hartley BS, Hanlon N, Jackson RJ, Rangarajan M (2000) Glucose isomerase: insights into protein engineering for increased thermostability. Biochim Biophys Acta 1543:294–335

    CAS  PubMed  Google Scholar 

  • Held D, Yeager K, Novy R (2003) New co-expression vectors for expanded compatibilities in E. coli. Innovations 18:4–6

    Google Scholar 

  • Johnson JC (1976) Sugar alcohols and derivatives. In: Specialized sugars for the food industry. Noyes Data Corporation, New Jersey, p 313

    Google Scholar 

  • Kaup B (2004) Gewinnung von d-Mannitol mit rekombinanten Escherichia coli Stämmen. Ph.D. thesis, Universität Düsseldorf, Germany

  • Kaup B, Bringer-Meyer S, Sahm H (2004) Metabolic engineering of Escherichia coli: construction of an efficient biocatalyst for d-mannitol formation in a whole-cell biotransformation. Appl Microbiol Biotechnol 64:333–339

    CAS  PubMed  Google Scholar 

  • Kornberg HL (2001) Routes for fructose utilization by Escherichia coli. J Mol Microbiol Biotechnol 3:355–359

    CAS  PubMed  Google Scholar 

  • Lawlis VB, Dennis MS, Chen EY, Smith DH, Henner DJ (1984) Cloning and sequencing of the xylose isomerase and xylulose kinase genes of Escherichia coli. Appl Environ Microbiol 47(1):15–21

    CAS  PubMed  PubMed Central  Google Scholar 

  • Makkee M, Kieboom APG, Bekkum H (1985) Production methods of d-mannitol. Starch 37(4):136–141

    CAS  Google Scholar 

  • Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 352–355

    Google Scholar 

  • Nidetzky B, Haltrich D, Schmidt K, Schmidt H, Weber A, Kulbe KD (1996) Simultaneous enzymatic synthesis of mannitol and gluconic acid. II. Development of a continuous process for a coupled NAD(H)-dependent enzyme system. Biocatal Biotransform 14:47–65

    CAS  Google Scholar 

  • Parker C, Barnell WO, Snoep JL, Ingram LO, Conway T (1995) Characterization of the Zymomonas mobilis glucose facilitator gene product (glf) in recombinant Escherichia coli: examination of transport mechanism, kinetics and the role of glucokinase in glucose transport. Mol Microbiol 15:759–802

    Google Scholar 

  • Rosenberg H, Pearce SM, Hardy CM, Jacomb PA (1984) Rapid turnover of mannitol-1-phosphate in Escherichia coli. J Bacteriol 158(1):63–68

    CAS  PubMed  PubMed Central  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  • Schellenberg GD, Sarthy A, Larson AE, Backer MP, Crabb JW, Lidstrom M, Hall BD, Furlong CE (1984) Xylose isomerase from Escherichia coli. Characterization of the protein and the structural gene. J Biol Chem 259(11):6826–6832

    CAS  PubMed  Google Scholar 

  • Schütte H, Flossdorf J, Sahm H, Kula MR (1976) Purification and properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii. Eur J Biochem 62:151–160

    Google Scholar 

  • Shamanna DK, Sanderson KE (1979a) Uptake and catabolism of d-xylose in Salmonella typhimurium LT2. J Bacteriol 139(1):64–70

    CAS  PubMed  PubMed Central  Google Scholar 

  • Shamanna DK, Sanderson KE (1979b) Genetics and regulation of d-xylose utilization in Salmonella typhimurium LT2. J Bacteriol 139(1):71–79

    CAS  PubMed  PubMed Central  Google Scholar 

  • Slatner M, Nagl G, Haltrich D, Kulbe KD, Nidetzky B (1998a) Enzymatic synthesis of mannitol. Reaction engineering for a recombinant mannitol dehydrogenase. Ann N Y Acad Sci 864:450–453

    CAS  PubMed  Google Scholar 

  • Slatner M, Nagl G, Haltrich D, Kulbe KD, Nidetzky B (1998b) Enzymatic production of pure d-mannitol at high productivity. Biocatal Biotransform 16:351–363

    CAS  Google Scholar 

  • Soetaert W, Buchholz K, Vandamme EJ (1995) Production of d-mannitol and d-lactic acid by fermentation with Leuconostoc mesenteroides. Agro Food Ind Hi-Tech 6:41–44

    CAS  Google Scholar 

  • Soetaert W, Vanhooren PT, Vandamme EJ (1999) The production of mannitol by fermentation. In: Bucke C (ed) Methods in biotechnology 10. Humana Press, Totowa, NJ, pp 261–275

    Google Scholar 

  • Studier FW, Moffat BA (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189:113–130

    CAS  PubMed  Google Scholar 

  • Tchieu JH, Norris V, Edwards JS, Saier MH Jr (2001) The complete phosphotranferase system in Escherichia coli. J Mol Microbiol Biotechnol 3:329–346

    CAS  PubMed  Google Scholar 

  • Thompson J (1987) Sugar transport in the lactic acid bacteria. In: Reizer J, Peterkofsky A (eds) Sugar transport and metabolism in gram-positive bacteria. Ellis Horwood Limited, Chichester, pp 13–38

    Google Scholar 

  • Van Bastelaere P, Vangrysperre W, Kersers-Hilderson H (1991) Kinetic studies of Mg2+-, Co2+- and Mn2+-activated d-xylose isomerases. Biochem J 278:285–292

    PubMed  PubMed Central  Google Scholar 

  • von Weymarn N, Kiviharju K, Leisola M (2002) High-level production of d-mannitol with membrane cell-recycle bioreactor. J Ind Microbiol Biotech 29:44–49

    Google Scholar 

  • Walton AZ, Stewart JD (2004) Understanding and improving NADPH-dependent reactions by nongrowing Escherichia coli cells. Biotechnol Prog 20(2):403–411

    CAS  PubMed  Google Scholar 

  • Weisser P, Krämer R, Sahm H, Sprenger GA (1995) Functional expression of the glucose transporter of Zymomonas mobilis leads to restoration of glucose and fructose uptake in Escherichia coli mutants and provides evidence for its facilitator action. J Bacteriol 177:3351–3354

    CAS  PubMed  PubMed Central  Google Scholar 

  • Wisselink HW, Weusthuis RA, Eggink G, Hugenholtz J, Grobben GJ (2002) Mannitol production by lactic acid bacteria: a review. Int Dairy J 12:151–161

    CAS  Google Scholar 

Download references

Acknowledgements

We wish to thank N. Esaki for plasmid pMcFDH, G.A. Sprenger for plasmid pZY507glf. This work was supported by the InnoSweet GmbH, Braunschweig, Germany and the Fonds der Chemischen Industrie.

Author information

Author notes

  1. Björn Kaup

    Present address: Karl-Winnacker-Institut, Bioverfahrenstechnik, DECHEMA e.V., Theodor-Heuss-Allee 25, 60486, Frankfurt, Germany

Authors and Affiliations

  1. Institut für Biotechnologie 1, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany

    Stephanie Bringer-Meyer & Hermann Sahm

Authors
  1. Björn Kaup
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Stephanie Bringer-Meyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hermann Sahm
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Stephanie Bringer-Meyer.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kaup, B., Bringer-Meyer, S. & Sahm, H. d-Mannitol formation from d-glucose in a whole-cell biotransformation with recombinant Escherichia coli . Appl Microbiol Biotechnol 69, 397–403 (2005). https://doi.org/10.1007/s00253-005-1996-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-005-1996-0

Keywords

Search

Navigation