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The use of genetically modified Saccharomyces cerevisiae strains in the wine industry

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Abstract

In recent decades, science and food technology have contributed at an accelerated rate to the introduction of new products to satisfy nutritional, socio-economic and quality requirements. With the emergence of modern molecular genetics, the industrial importance of Saccharomyces cerevisiae, is continuously extended. The demand for suitable genetically modified (GM) S. cerevisiae strains for the biofuel, bakery and beverage industries or for the production of biotechnological products (e.g. enzymes, pharmaceutical products) will continuously grow in the future. Numerous specialised S. cerevisiae wine strains were obtained in recent years, possessing a wide range of optimised or novel oenological properties, capable of satisfying the demanding nature of modern winemaking practise. The unlocking of transcriptome, proteome and metabolome complexities will contribute decisively to the knowledge about the genetic make-up of commercial yeast strains and will influence wine strain improvement via genetic engineering. The most relevant advances regarding the importance and implications of the use of GM yeast strains in the wine industry are discussed in this mini-review. In this work, various aspects are considered including the strategies used for the construction of strains with respect to current legislation requirements, the environmental risk evaluations concerning the deliberate release of genetically modified yeast strains, the methods for detection of recombinant DNA and protein that are currently under evaluation, and the reasons behind the critical public perception towards the application of such strains.

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References

  • Ahmed FE (2002) Detection of genetically modified organisms in foods. Trends Biotechnol 20:215–223

    Google Scholar 

  • Akada R (2002) Genetically modified industrial yeast ready for application. J Biosci Bioeng 94:536–544

    Google Scholar 

  • Akada R, Matsuo K, Aritomi K, Nishizawa Y (1999) Construction of recombinant sake yeast containing a dominant FAS2 mutation without extraneous sequences by a two-step gene replacement protocol. J Biosci Bioeng 87:43–48

    Google Scholar 

  • Anonymous (2001) Europeans, Science and Technology (Eurobarometer 55.2), Brussels, European Opinion Research Group, available (April 2005) at http://europa.eu.int/comm/research/press/2001/pr0612en-report.pdf

  • Aritomi K, Hirosawa I, Hoshida H, Shiigi M, Nishizawa Y, Kashiwagi S, Akada R (2004) Self-cloning yeast strains containing novel FAS2 mutations produce a higher amount of ethyl caproate in Japanese sake. Biosci Biotechnol Biochem 68:206–214

    Google Scholar 

  • Auer CA (2003) Tracking genes from seed to supermarket: techniques and trends. Trends Plant Sci 8:591–597

    Google Scholar 

  • Bauer F, Dequin S, Pretorius I, Shoeman H, Wolfaardt MB, Schroeder MB, Grossmann MK (2003) The assessment of the environmental impact of genetically modified wine yeast strains. Proceedings of the “Actes de 83ème Assemblée Générale de l’O.I.V”

  • Becker JVW, Armstrong GO, Van der Merwe MJ, Lambrechts MG, Vivier MA, Pretorius IS (2003) Metabolic engineering of Saccharomyces cerevisiae for the synthesis of the wine-related antioxidant resveratrol. FEMS Yeast Res 4:79–85

    Google Scholar 

  • Bendoni B, Cavalieri D, Casalone E, Polsinelli M, Barberio C (1999) Trifluoroleucine resistance as a dominant molecular marker in transformation of strains of Saccharomyces cerevisiae isolated from wine. FEMS Microbiol Lett 180:229–233

    Google Scholar 

  • Blondin B, Dequin S (1998) Yeast, wine and genetic engineering. Biofutur 182:16–20

    Google Scholar 

  • Brandolini V, Tedeschi P, Capece A, Maietti A, Mazzotta D, Salzano G, Paparella A, Romano P (2002) Saccharomyces cerevisiae wine strains differing in copper resistance exhibit different capability to reduce copper content in wine. World J Microbiol Biotechnol 18:499–503

    Google Scholar 

  • Brett GM, Chambers SJ, Huang L, Morgan MRA (1999) Design and development of immunoassays for detection of proteins. Food Control 10:401–406

    Google Scholar 

  • Caridi A, Cufari A, Ramondino D (2002) Isolation and clonal pre-selection of enological Saccharomyces. J Gen Appl Microbiol 48:261–267

    Google Scholar 

  • Carstens M, Vivier MA, Van Rensburg P, Pretorius IS (2003) Overexpression, secretion and antifungal activity of the Saccharomyces cerevisiae chitinase. Ann Microbiol 53:15–28

    Google Scholar 

  • Cavalieri D, McGovern PE, Hartl DL, Mortimer R, Polsinelli M (2003) Evidence for S. cerevisiae fermentation in ancient wine. J Mol Evol 57:S226–S232

    Google Scholar 

  • Ciani M, Maccarelli F (1998) Oenological properties of non-Saccharomyces yeasts associated with wine-making. World J Microbiol Biotechnol 14:199–203

    Google Scholar 

  • Clemente-Jimenez JM, Mingorance-Cazorla L, Martinez-Rodriguez S, Las Heras-Vazquez FJ, Rodriguez-Vico F (2004) Molecular characterization and oenological properties of wine yeasts isolated during spontaneous fermentation of six varieties of grape must. Food Microbiol 21:149–155

    Google Scholar 

  • Corpillo D, Gardini G, Vaira AM, Basso M, Aime S, Accotto GR, Fasano M (2004) Proteomics as a tool to improve investigation of substantial equivalence in genetically modified organisms: the case of a virus-resistant tomato. Proteomics 4:193–200

    Google Scholar 

  • Dequin S (2001) The potential of genetic engineering for improving brewing, wine-making and baking yeasts. Appl Microbiol Biotechnol 56:577–588

    Google Scholar 

  • Dequin S, Baptista E, Barre P (1999) Acidification of grape musts by Saccharomyces cerevisiae wine yeast strains genetically engineered to produce lactic acid. Am J Enol Vitic 50:45–50

    Google Scholar 

  • Dequin S, Salmon JM, Nguyen HV, Blondin B (2003) Wine yeasts. In: Boekhout T, Robert B (eds) Yeasts in food, beneficial and detrimental aspects. Berhr’s Verlag, Hamburg, Germany, pp 389–412

    Google Scholar 

  • DIN (2002a) (Norm-Entwurf) DIN EN ISO 21569, Ausgabe:2002-12. Lebensmittel-Verfahren zum Nachweis von gentechnisch modifizierten Organismen und ihren Produkten-Qualitative auf Nukleinsäuren basierende Verfahren (ISO/DIS 21569:2002); Deutsche Fassung prEN ISO 21569:2002. Deutsches Institut für Normung

  • DIN (2002b) (Norm-Entwurf) DIN EN ISO 21571, Ausgabe:2002-12. Lebensmittel - Verfahren zum Nachweis von gentechnisch modifizierten Organismen und ihren Produkten - Nukleinsäureextraktion (ISO/DIS 21571:2002); Deutsche Fassung prEN ISO 21571:2002. Deutsches Institut für Normung

  • DIN (2002c) (Norm-Entwurf) DIN EN ISO 21572, Ausgabe:2002–03. Lebensmittel-Nachweis von gentechnisch veränderten Organismen und daraus hergestellten Erzeugnissen-Proteinverfahren (ISO/DIS 21572:2002); Deutsche Fassung prEN ISO 21572:2002. Deutsches Institut für Normung

  • DIN (2003) (Norm-Entwurf) DIN EN ISO 21568, Ausgabe: 2003-07. Lebensmittel-Verfahren zum Nachweis von gentechnisch modifizierten Organismen und ihren Produkten-Probenahme (ISO/DIS 21568:2003); Deutsche Fassung prEN ISO 21568:2003. Deutsches Institut für Normung

  • du Toit C, Pretorius I (2000) Microbial spoilage and preservation of wine: using new weapons from nature’s own arsenal—a review. S Afr J Enol Vitic 21:74–96

    Google Scholar 

  • EC (1990) Directive 90/220/EEC of the European Council of 23 April 1990 on the deliberate release into the environment of genetically modified organisms. Official Journal of the European Communities L117, 08.05.1990:15–27

  • EC (1997) Regulation (EC) No 258/97 of the European Parliament and of the Council of 27 January 1997 concerning novel foods and novel food ingredients. Official Journal of the European Communities L43, 14.02.1997:1–6

  • EC (2001) Directive 2001/18/EC of the European Parliament and of the Council of 12 March 2001 on the deliberate release into the environment of genetically modified organisms and repealing Council Directive 90/220/EC. Official Journal of the European Communities L106, 12.3.2001:1–38

  • EC (2002) Commission Decision 2002/623/EC of 24 July 2002 establishing guidance noted supplementing Annex II to Directive 2001/18/EC of the European Parliament and of the Council of 12 March 2001 on the deliberate release into the environment of genetically modified organisms and repealing Council Directive 90/220/EC. Official Journal of the European Communities L200, 30.7.2002:20–32

    Google Scholar 

  • EC (2003a) Regulation (EC) No 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed. Official Journal of the European Communities L268, 18.10.2003:1–23

  • EC (2003b) Regulation (EC) No 1830/2003 of the European Parliament and of the Council of 22 September 2003 concerning the traceability and labelling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending Directive 2001/18/EC. Official Journal of the European CommunitiesL268, 18.10.2003:24–28

  • EC (2004) Regulation (EC) No 65/2004 of 14 January 2004 establishing a system for the development and assignment of unique identifiers for genetically modified organisms. Official Journal of the European Communities L10, 16.1.2004:5–10

  • Esteve-Zarzoso B, Manzanares P, Ramon D, Querol A (1998) The role of non-Saccharomyces yeasts in industrial winemaking. Int Microbiol 1:143–148

    Google Scholar 

  • Esteve-Zarzoso B, Gostincar A, Bobet R, Uruburu F, Querol A (2000) Selection and molecular characterization of wine yeasts isolated from the ‘El Penedes’ area (Spain). Food Microbiol 17:553–562

    Google Scholar 

  • Fernandez M, Ubeda JF, Briones AI (2000) Typing of non-Saccharomyces yeasts with enzymatic activities of interest in wine-making. Int J Food Microbiol 59:29–36

    Google Scholar 

  • Fleet GH, Heard GM (1993) Yeasts: growth during fermentation. In: Fleet GH (ed) Wine microbiology and biotechnology. Harwood, Philadelphia, PA, pp 27–55

    Google Scholar 

  • Frewer L (2003) Societal issues and public attitudes towards genetically modified foods. Trends Food Sci Technol 14:319–332

    Google Scholar 

  • Frewer L, Howard C, Shepherd R (1997) Public concerns about general and specific applications of genetic engineering: risk, benefit and ethics. Sci Technol Human Values 22:98–124

    Google Scholar 

  • Ganga MA, Pinaga F, Valles S, Ramon D, Querol A (1999) Aroma improving in microvinification processes by the use of a recombinant wine yeast strain expressing the Aspergillus nidulans xlnA gene. Int J Food Microbiol 47:171–178

    Google Scholar 

  • Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG (1996) Life with 6000 genes. Science 274(5287):546, 563-7

    Google Scholar 

  • Gonzalez-Candelas L, Cortell A, Ramon D (1995) Construction of a recombinant wine yeast strain expressing a fungal pectate lyase gene. FEMS Microbiol Lett 126:263–269

    Google Scholar 

  • Gonzalez-Candelas L, Gil JV, Lamuela-Raventos RM, Ramon D (2000) The use of transgenic yeasts expressing a gene encoding a glycosyl-hydrolase as a tool to increase resveratrol content in wine. Int J Food Microbiol 59:179–183

    Google Scholar 

  • Granchi L, Ganucci D, Messini A, Vincenzini M (2002) Oenological properties of Hanseniaspora osmophila and Kloeckera corticis from wines produced by spontaneous fermentations of normal and dried grapes. FEMS Yeast Res 2:403–407

    Google Scholar 

  • Guerra E, Mannazzu I, Sordi G, Tangherlini M, Clementi F, Fatichenti F (1999) Characterization of indigenous Saccharomyces cerevisiae from the Italian region of Marche: hunting for new strains for local wine quality improvement. Ann Microbiol Enzimol 49:79–88

    Google Scholar 

  • Hübner P, Studer E, Lüthy J (1999) Quantitative competitive PCR for the detection of genetically modified organisms in food. Food Control 10:353–358

    Google Scholar 

  • ILSI (1998) Detection methods for novel foods derived from genetically modified organisms. International Life Science Institute Europe, Brussels

  • ILSI (1999) Safety assessment of viable genetically modified microorganisms used in food—consensus guidelines reached at a workshop held in April 1999. Brussels International Life Science Institute Europe, Novel Food Task Force

  • ILSI (2001) Method development in relation to regulatory requirements for the detection of GMOs in the food chain—summary report of a joint workshop held in December 2000; Prepared by the International Life Science Institute Novel Food Task Force in collaboration with the European Commission’s Joint Research Centr (JRC) and ILSI International Food Biotechnology Committee

  • Kuiper HA, Kleter GA (2003) The scientific basis for risk assessment and regulation of genetically modified foods. Trends Food Sci Technol 14:277–293

    Google Scholar 

  • Kuiper HA, Kleter GA, Noteborn H, Kok EJ (2002) Substantial equivalence—an appropriate paradigm for the safety assessment of genetically modified foods? Toxicology 181:427–431

    Google Scholar 

  • Lilly M, Lambrechts MG, Pretorius IS (2000) Effect of increased yeast alcohol acetyltransferase activity on flavor profiles of wine and distillates. Appl Environ Microbiol 66:744–753

    Google Scholar 

  • Liu G, Su W, Xu Q, Long M, Zhou J, Song S (2004) Liquid-phase hybridization based PCR-ELISA for detection of genetically modified organisms in food. Food Control 15:303–306

    Google Scholar 

  • Lüthy J (1999) Detection strategies for food authenticity and genetically modified foods. Food Control 10:359–361

    Google Scholar 

  • Maifreni M, Comi G, Rondinini G (1999) Selection and oenological characterisation of Saccharomyces cerevisiae strains isolated from Tocai, Pinot and Malvasia grapes and musts of the Collio area. Ann Microbiol Enzimol 49:33–43

    Google Scholar 

  • Malherbe DF, du Toit M, Otero RRC, van Rensburg P, Pretorius IS (2003) Expression of the Aspergillus niger glucose oxidase gene in Saccharomyces cerevisiae and its potential applications in wine production. Appl Microbiol Biotechnol 61:502–511

    Google Scholar 

  • Mannazzu I, Clementi F, Ciani M (2002) Strategies and criteria for the isolation and selection of autochthonous starters. In: Ciani M (ed) Biodiversity and biotechnology of wine yeasts. Research Signpost, Trivandrum, India, pp 19–35

    Google Scholar 

  • Manzanares P, Orejas M, Gil JV, de Graaff LH, Visser J, Ramon D (2003) Construction of a genetically modified wine yeast strain expressing the Aspergillus aculeatus rhaA gene, encoding an alpha-l-rhamnosidase of enological interest. Appl Environ Microbiol 69:7558–7562

    Google Scholar 

  • Marinoni G, Manuel M, Petersen RF, Hvidtfeldt J, Sulo P, Piskur J (1999) Horizontal transfer of genetic material among Saccharomyces yeasts. J Bacteriol 181:6488–6496

    Google Scholar 

  • Martinez-Rodriguez A, Carrascosa AV, Barcenilla JM, Pozo-Bayon MA, Polo MC (2001) Autolytic capacity and foam analysis as additional criteria for the selection of yeast strains for sparkling wine production. Food Microbiol 18:183–191

    Google Scholar 

  • Mendes-Ferreira A, Mendes-Faia A, Leão C (2002) Survey of hydrogen sulphide production by wine yeasts. J Food Prot 65:1033–1037

    Google Scholar 

  • Meyer R (1999) Development and application of DNA analytical methods for the detection of GMOs in food. Food Control 10:391–399

    Google Scholar 

  • Michnick S, Roustan JL, Remize F, Barre P, Dequin S (1997) Modulation of glycerol and ethanol yields during alcoholic fermentation in Saccharomyces cerevisiae strains overexpressed or disrupted for GPD1 encoding glycerol 3-phosphate dehydrogenase. Yeast 13:783–793

    Google Scholar 

  • Mingorance-Cazorla L, Clemente-Jimenez JM, Martinez-Rodriguez S, Las Heras-Vazquez FJ, Rodriguez-Vico F (2003) Contribution of different natural yeasts to the aroma of two alcoholic beverages. World J Microbiol Biotechnol 19:297–304

    Google Scholar 

  • Moseley BEB (1999) The safety and social acceptance of novel foods. Int J Food Microbiol 50:25–31

    Google Scholar 

  • Nevoigt E, Fassbender A, Stahl U (2000) Cells of the yeast Saccharomyces cerevisiae are transformable by DNA under non-artificial conditions. Yeast 16:1107–1110

    Google Scholar 

  • Otero RRC, Iranzo JFU, Briones-Perez AI, Potgieter N, Villena MA, Pretorius IS, van Rensburg P (2003) Characterization of the beta-glucosidase activity produced by enological strains of non-Saccharomyces yeasts. J Food Sci 68:2564–2569

    Google Scholar 

  • Patel S, Shibamoto T (2003) Effect of 20 different yeast strains on the production of volatile components in Symphony wine. J Food Compos Anal 16:469–476

    Google Scholar 

  • Perez-Coello MS, Perez AIB, Iranzo JFU, Alvarez PJM (1999) Characteristics of wines fermented with different Saccharomyces cerevisiae strains isolated from the La Mancha region. Food Microbiol 16:563–573

    Google Scholar 

  • Pérez-González JA, Gonzalez R, Querol A, Sendra J, Ramon D (1993) Construction of a recombinant wine yeast strain expressing β-(1,4)-endoglucanase and its use in microvinification processes. Appl Environ Microbiol 59:2801–2806

    Google Scholar 

  • Petit L, Baraige F, Balois AM, Bertheau Y, Fach P (2003) Screening of genetically modified organisms and specific detection of Bt176 maize in flours and starches by PCR-enzyme linked immunosorbent assay. Eur Food Res Technol 217:83–89

    Google Scholar 

  • Plata C, Millan C, Mauricio JC, Ortega JM (2003) Formation of ethyl acetate and isoamyl acetate by various species of wine yeasts. Food Microbiol 20:217–224

    Google Scholar 

  • Pretorius IS (2000) Tailoring wine yeast for the new millennium: novel approaches to the ancient art of winemaking. Yeast 16:675–729

    Google Scholar 

  • Pretorius IS, Bauer FF (2002) Meeting the consumer challenge through genetically customized wine-yeast strains. Trends Biotechnol 20:426–432

    Google Scholar 

  • Pretorius IS, du Toit M, van Rensburg P (2003) Designer yeasts for the fermentation industry of the 21st century. Food Technol Biotechnol 41:3–10

    Google Scholar 

  • Rainieri S, Pretorius IS (2000) Selection and improvement of wine yeasts. Ann Microbiol 50:15–31

    Google Scholar 

  • Regodon JA, Perez F, Valdes ME, DeMiguel C, Ramirez M (1997) A simple and effective procedure for selection of wine yeast strains. Food Microbiol 14:247–254

    Google Scholar 

  • Remize F, Roustan JL, Sablayrolles JM, Barre P, Dequin S (1999) Glycerol overproduction by engineered Saccharomyces cerevisiae wine yeast strains leads to substantial changes in by-product formation and to a stimulation of fermentation rate in stationary phase. Appl Environ Microbiol 65:143–149

    Google Scholar 

  • Remize F, Andrieu E, Dequin S (2000) Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae: role of the cytosolic Mg2+ and mitochondrial K+ acetaldehyde dehydrogenases Ald6p and Ald4p in acetate formation during alcoholic fermentation. Appl Environ Microbiol 66:3151–3159

    Google Scholar 

  • Restuccia C, Pulvirenti A, Caggia C, Giudici P (2002) A beta-glucosidase positive strain of Saccharomyces cerevisiae isolated from grape must. Ann Microbiol 52:47–53

    Google Scholar 

  • Rodriguez ME, Lopes CA, Broock M, Valles S, Ramon D, Caballero AC (2004) Screening and typing of Patagonian wine yeasts for glycosidase activities. J Appl Microbiol 96:84–95

    Google Scholar 

  • Rogan GJ, Dudin YA, Lee TC, Magin KM, Astwood JD, Bhakta NS, Leach JN, Sanders PR, Fuchs RL (1999) Immunodiagnostic methods for detection of 5-enolpyruvylshikimate-3-phosphate synthase in Roundup Ready soybeans. Food Control 10:407–414

    Google Scholar 

  • Romano P, Monteleone E, Paraggio M, Marchese R, Caporale G, Carlucci A (1998) A methodological approach to the selection of Saccharomyces cerevisiae wine strains. Food Technol Biotechnol 36:69–74

    Google Scholar 

  • Romano P, Caruso M, Capece A, Lipani G, Paraggio M, Fiore C (2003a) Metabolic diversity of Saccharomyces cerevisiae strains from spontaneously fermented grape musts. World J Microbiol Biotechnol 19:311–315

    Google Scholar 

  • Romano P, Fiore C, Paraggio M, Caruso M, Capece A (2003b) Function of yeast species and strains in wine flavour. Int J Food Microbiol 86:169–180

    Google Scholar 

  • Romano P, Granchi L, Caruso M, Borra G, Palla G, Fiore C, Ganucci D, Caligiani A, Brandolini V (2003c) The species-specific ratios of 2,3-butanediol and acetoin isomers as a tool to evaluate wine yeast performance. Int J Food Microbiol 86:163–168

    Google Scholar 

  • Ronning SB, Vaitilingom M, Berdal KG, Holst-Jensen A (2003) Event specific real-time quantitative PCR for genetically modified Bt11 maize (Zea mays). Eur Food Res Technol 216:347–354

    Google Scholar 

  • Rudi K, Rud I, Holck A (2003) A novel multiplex quantitative DNA array based PCR (MQDA-PCR) for quantification of transgenic maize in food and feed. Nucleic Acids Res 31(11):e62

    Google Scholar 

  • Salmon JM, Barre P (1998) Improvement of nitrogen assimilation and fermentation kinetics under enological conditions by derepression of alternative nitrogen-assimilatory pathways in an industrial Saccharomyces cerevisiae strain. Appl Environ Microbiol 64:3831–3837

    Google Scholar 

  • Sanchez-Torres P, Gonzalez-Candelas L, Ramon D (1996) Expression in a wine yeast strain of the Aspergillus niger abfB gene. FEMS Microbiol Lett 145:189–194

    Google Scholar 

  • Schoeman H, Vivier MA, Du Toit M, Dicks LMT, Pretorius IS (1999) The development of bactericidal yeast strains by expressing the Pediococcus acidilactici pediocin gene (pedA) in Saccharomyces cerevisiae. Yeast 15:647–656

    Google Scholar 

  • Silva S, Ramon-Portugal F, Andrade P, Abreu S, Texeira MD, Strehaiano P (2003) Malic acid consumption by dry immobilized cells of Schizosaccharomyces pombe. Am J Enol Vitic 54:50–55

    Google Scholar 

  • Smit A, Otero RRC, Lambrechts MG, Pretorius IS, Van Rensburg P (2003) Enhancing volatile phenol concentrations in wine by expressing various phenolic acid decarboxylase genes in Saccharomyces cerevisiae. J Agric Food Chem 51:4909–4915

    Google Scholar 

  • Spiropoulos A, Tanaka J, Flerianos I, Bisson LF (2000) Characterization of hydrogen sulfide formation in commercial and natural wine isolates of Saccharomyces. Am J Enol Vitic 51:233–248

    Google Scholar 

  • Stave JW (1999) Detection of new or modified proteins in novel foods derived from GMO—future needs. Food Control 10:367–374

    Google Scholar 

  • Steger CLC, Lambrechts MG (2000) The selection of yeast strains for the production of premium quality South African brandy base products. J Ind Microbiol Biotechnol 24:431–440

    Google Scholar 

  • Sutherland CM, Henschke PA, Langridge P, Lopes MD (2003) Subunit and cofactor binding of Saccharomyces cerevisiae sulfite reductase—towards developing wine yeast with lowered ability to produce hydrogen sulfide. Aust J Grape Wine Res 9:186–193

    Google Scholar 

  • Toro ME, Vazquez F (2002) Fermentation behaviour of controlled mixed and sequential cultures of Candida cantarellii and Saccharomyces cerevisiae wine yeasts. World J Microbiol Biotechnol 18:347–354

    Google Scholar 

  • Uzogara SG (2000) The impact of genetic modification of human foods in the 21st century: a review. Biotechnol Adv 18:179–206

    Google Scholar 

  • Vaitilingom M, Pijnenburg H, Gendre F, Brignon P (1999) Real-time quantitative PCR detection of genetically modified maximizer maize and roundup ready soybean in some representative foods. J Agric Food Chem 47:5261–5266

    Google Scholar 

  • Valero E, Schuller D,Cambon B,Casal M, Dequin S (2005) Dissemination and survival of commercial wine yeast in the vineyard: a large-scale, three years study. FEMS Yeast Research (in press)

    Google Scholar 

  • van Duijn G, van Biert R, Bleeker-Marcelis H, Peppelman H, Hessing M (1999) Detection methods for genetically modified crops. Food Control 10:375–378

    Google Scholar 

  • van Duijn G, van Biert R, Bleeker-Marcelis H, van Boeijen I, Adan AJ, Jhakrie S, Hessing M (2002) Detection of genetically modified organisms in foods by protein- and DNA-based techniques: bridging the methods. J AOAC Int 85:787–791

    Google Scholar 

  • Vilanova M, Blanco P, Cortes S, Castro M, Villa TG, Sieiro C (2000) Use of a PGU1 recombinant Saccharomyces cerevisiae strain in oenological fermentations. J Appl Microbiol 89:876–883

    Google Scholar 

  • Volschenk H, Viljoen M, Grobler J, Petzold B, Bauer F, Subden RE, Young RA, Lonvaud A, Denayrolles M, vanVuuren HJJ (1997) Engineering pathways for malate degradation in Saccharomyces cerevisiae. Nat Biotechnol 15:253–257

    Google Scholar 

  • Volschenk H, Viljoen-Bloom M, Subden RE, van Vuuren HJJ (2001) Malo-ethanolic fermentation in grape must by recombinant strains of Saccharomyces cerevisiae. Yeast 18:963–970

    Google Scholar 

  • Zhao J, Fleet GH (2003) Degradation of DNA during the autolysis of Saccharomyces cerevisiae. J Ind Microbiol Biotechnol 30:175–182

    Google Scholar 

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Acknowledgements

This study was supported by the portuguese grants ENOSAFE (No. 762, Programa AGRO, medida 8) Instituto Nacional de Investigação Agrária and Pocti/Bio/38106/2001 (Eixo 2, Medida 2.3, QCA III - FEDER).

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  1. Centro de Biologia (CB-UM), Departamento de Biologia, Universidade do Minho, 4710-057, Braga, Portugal

    Dorit Schuller & Margarida Casal

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  1. Dorit Schuller
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Correspondence to Dorit Schuller.

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Schuller, D., Casal, M. The use of genetically modified Saccharomyces cerevisiae strains in the wine industry. Appl Microbiol Biotechnol 68, 292–304 (2005). https://doi.org/10.1007/s00253-005-1994-2

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