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The solvent-tolerant Pseudomonas putida S12 as host for the production of cinnamic acid from glucose

  • Applied Genetics and Molecular Biotechnology
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Abstract

A Pseudomonas putida S12 strain was constructed that efficiently produced the fine chemical cinnamic acid from glucose or glycerol via the central metabolite phenylalanine. The gene encoding phenylalanine ammonia lyase from the yeast Rhodosporidium toruloides was introduced. Phenylalanine availability was the main bottleneck in cinnamic acid production, which could not be overcome by the overexpressing enzymes of the phenylalanine biosynthesis pathway. A successful approach in abolishing this limitation was the generation of a bank of random mutants and selection on the toxic phenylalanine anti-metabolite m-fluoro-phenylalanine. Following high-throughput screening, a mutant strain was obtained that, under optimised culture conditions, accumulated over 5 mM of cinnamic acid with a yield (Cmol%) of 6.7%.

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Authors and Affiliations

  1. TNO Quality of Life, Business Unit Bioconversion and Processes for Food Industry, Apeldoorn, The Netherlands

    Karin Nijkamp, Jan A. M. de Bont & Jan Wery

  2. TNO Quality of Life, Business Unit Microbiology, Zeist, The Netherlands

    Nicole van Luijk

  3. PO Box 342, Apeldoorn, 7300, The Netherlands

    Karin Nijkamp

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  1. Karin Nijkamp
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  2. Nicole van Luijk
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  3. Jan A. M. de Bont
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  4. Jan Wery
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Correspondence to Karin Nijkamp.

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Nijkamp, K., van Luijk, N., de Bont, J.A.M. et al. The solvent-tolerant Pseudomonas putida S12 as host for the production of cinnamic acid from glucose. Appl Microbiol Biotechnol 69, 170–177 (2005). https://doi.org/10.1007/s00253-005-1973-7

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  • DOI: https://doi.org/10.1007/s00253-005-1973-7

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