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Use of fluorescence spectroscopy to differentiate yeast and bacterial cells

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Abstract

This study focuses on the characterization of bacterial and yeast species through their autofluorescence spectra. Lactic acid bacteria (Lactobacillus sp.), and yeast (Saccharomyces sp.) were cultured under controlled conditions and studied for variations in their autofluorescence, particularly in the area representative of tryptophan residues of proteins. The emission and excitation spectra clearly reveal that bacterial and yeast species can be differentiated by their intrinsic fluorescence with UV excitation. The possibility of differentiation between different strains of Saccharomyces yeast was also studied, with clear differences observed for selected strains. The study shows that fluorescence can be successfully used to differentiate between yeast and bacteria and between different yeast species, through the identification of spectroscopic fingerprints, without the need for fluorescent staining.

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References

  • Amiel C, Mariey L, Curk-Daubie M-C, Pichon P, Travert J (2000) Potentiality of Fourier transform infrared spectroscopy (FTIR) for discrimination and identification of dairy lactic acid bacteria. Lait 80:445–459

    Article  CAS  Google Scholar 

  • Barnett JA, Payne RW, Yarrow D (1990) Yeasts: characteristics and identification, 2nd edn. Cambridge Univ. Press, Cambridge

    Google Scholar 

  • Bayrock D, Ingledew WM (2001) Changes in steady state on introduction of a Lactobacillus contaminant to a continuous culture ethanol fermentation. J Ind Microbiol Biotechnol 27:39–45

    Article  CAS  Google Scholar 

  • Berthier F, Ehrlich SD (1998) Rapid species identification within two groups of closely related lactobacilli using PCR primers that target the 16S/23S rRNA spacer region. FEMS Microbiol Lett 161:97–106

    Article  CAS  Google Scholar 

  • Davis JG, McLachlan T (1974) Yogurt in the United Kingdom: chemical and microbiological analysis. Dairy Ind 39:149–177

    CAS  Google Scholar 

  • Edwards CG, Reynolds AG, Rodriguez AV, Semon MJ, Mills JM (1999) Implication of acetic acid in the induction of slow/stuck grape juice fermentations and inhibition of yeast by Lactobacillus sp. Am J Enol Vitic 50:204–210

    CAS  Google Scholar 

  • Giana HE, Silveira L Jr, Zangaro RA, Pacheco MTT (2003) Rapid identification of bacterial species by fluorescence spectroscopy and classification through principal components analysis. J Fluoresc 13:489–493

    Article  CAS  Google Scholar 

  • Gunasekera TS, Attfield PV, Veal DA (2000) A flow cytometry method for rapid detection and enumeration of total bacteria in milk. Appl Environ Microbiol 66:1228–1232

    Article  CAS  Google Scholar 

  • Ivnitski D, Abdel-Hamid I, Atanasov P, Wilkins E (1999) Biosensors for detection of pathogenic bacteria. Biosens Bioelectron 14:599–624

    Article  CAS  Google Scholar 

  • Jolliffe I (2002) Principle component analysis, 2nd edn. Springer series in statistics. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Kirschner C, Maquelin K, Pina P, Ngo Thi NA, Choo-Smith LP, Sockalingum GD, Sandt C, Ami D, Orsini F, Doglia SM, Allouch P, Mainfait M, Puppels GJ, Naumann D (2001) Classification and identification of enterococci: a comparative phenotypic, genotypic, and vibrational spectroscopic study. J Clin Microbiol 39:1763–1770

    Article  CAS  Google Scholar 

  • Kosse D, Ostenrieder I, Seiler H, Scherer S (1998) Rapid detection and identification of yeasts in yogurt using fluorescently labeled oligonucleotide probes. Int Dairy Fed spec issue 9801:132–137

    CAS  Google Scholar 

  • Lakowicz JR (1999) Principles of fluorescence spectroscopy, 2nd edn. Kluwer Academic/Plenum Publishers, New York

    Book  Google Scholar 

  • Leblanc L, Dufour E (2002) Monitoring the identity of bacteria using their intrinsic fluorescence. FEMS Microbiol Lett 211:147–153

    Article  CAS  Google Scholar 

  • Lefier D, Lamprell H, Mazerolles G (2000) Evolution of Lactococcus strains during ripening in Brie cheese using Fourier transform infrared spectroscopy. Lait 80:247–254

    Article  CAS  Google Scholar 

  • Lehninger AL, Nelson DL, Cox MM (1993) Principles of biochemistry, 2nd edn. Worth Publishers, New York

    Google Scholar 

  • Lewis JG, Learmonth RP, Attifield PV, Watson K (1997) Stress co-tolerance and trehalose content in baking strains of Saccharomyces cerevisiae. J Ind Microbiol Biotech 18:30–36

    Article  CAS  Google Scholar 

  • Liu SQ, Holland R, Crow VL (2004) Esters and their biosynthesis in fermented dairy products: a review. Int Dairy J 14:923–945

    Article  CAS  Google Scholar 

  • Lonvaud-Funel A (1999) Lactic acid bacteria in the quality improvement and depreciation of wine. Antonie Van Leeuwenhoek 76:317–331

    Article  CAS  Google Scholar 

  • Van Reenen CA, Dicks LM (1996) Evaluation of numerical analysis of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate Lactobacillus plantarum and Lactobacillus pentosus. Curr Microbiol 32:183–187

    Article  Google Scholar 

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Acknowledgements

The authors would like to acknowledge the support of grants from the Australian Research Council and the National Health and Medical Research Council Research Network for Fluorescence Applications in Biotechnology and Life Sciences (RN0460002), Macquarie University Research & Development Scheme, and the University of Southern Queensland Centre for Rural & Environmental Biotechnology.

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Authors and Affiliations

  1. Department of Physics, Division of Information and Communication Sciences, Macquarie University, North Ryde, NSW 2109, Australia

    Hemant Bhatta & Ewa M. Goldys

  2. Centre for Systems Biology and Department of Biological and Physical Sciences, University of Southern Queensland, Toowoomba, QLD 4350, Australia

    Robert P. Learmonth

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  1. Hemant Bhatta
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  2. Ewa M. Goldys
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  3. Robert P. Learmonth
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Correspondence to Hemant Bhatta.

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Bhatta, H., Goldys, E.M. & Learmonth, R.P. Use of fluorescence spectroscopy to differentiate yeast and bacterial cells. Appl Microbiol Biotechnol 71, 121–126 (2006). https://doi.org/10.1007/s00253-005-0309-y

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  • DOI: https://doi.org/10.1007/s00253-005-0309-y

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