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A serum-free medium for colony growth and hyaluronic acid production by Streptococcus zooepidemicus NJUST01

  • Applied Microbial and Cell Physiology
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Abstract

A hyaluronic acid (HA)-producing strain, Streptococcus zooepidemicus NJUST01, can grow in a serum-free agar medium, with starch as exclusive carbon source, but not glucose, sucrose, dextrine, xylose, or lactose. In this starch medium, the strain NJUST01 reproduced successively at 37°C for 60 generations, with no obvious variation on morphology and physiology, but colonies of the strain after 60th generation could not produce a clear hemolytic zone on sheep blood agar plates. Hyaluronic acid production by the strain NJUST01 was analyzed relative to the starch medium. Employing a multifactor cross experiment, an optimum medium revealed for hyaluronic acid production was composed of 5% starch, 0.3% glucose, 0.5% peptone, 0.15% MgSO4, and 2.0% K2HPO4. The amount of HA 6.7 g/l was obtained in batch fermentation on a rotary shaker at 37°C, 220 rpm for 36 h.

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Acknowledgement

A grant/contract sponsorship from Shedian Corporation, China, is acknowledged.

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Authors and Affiliations

  1. State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Nanjing, 210093, People’s Republic of China

    Jianfa Zhang, Liuyan Yang & Zhiming Kong

  2. Department of Biochemical Engineering, Nanjing University of Science & Technology, Nanjing, 210094, People’s Republic of China

    Jianfa Zhang & Xia Ding

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  1. Jianfa Zhang
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  2. Xia Ding
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  3. Liuyan Yang
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  4. Zhiming Kong
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Correspondence to Jianfa Zhang.

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Zhang, J., Ding, X., Yang, L. et al. A serum-free medium for colony growth and hyaluronic acid production by Streptococcus zooepidemicus NJUST01. Appl Microbiol Biotechnol 72, 168–172 (2006). https://doi.org/10.1007/s00253-005-0253-x

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  • DOI: https://doi.org/10.1007/s00253-005-0253-x

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