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Generation of aroma compounds from Ditaxis heterantha by Saccharomyces cerevisiae

  • Applied Microbial and Cell Physiology
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Abstract

Ditaxis heterantha, a plant of the Euphorbiaceae family, is growing wild in the semiarid regions of Mexico. The seed endosperm contains yellow pigments (carotenoids). By high-pressure liquid chromatography the total pigment (TP) was separated into seven fractions: two of them, heterathin (F4) and ditaxin (F5), characterized as apocarotenoids, represent 80% of TP. Both molecules have double bonds, which seem to be the target for degradation and aroma formation. In this work, TP, F4, and F5 were supplied to nine cultures able to degrade lutein. From these strains, only one (identified as Saccharomyces cerevisiae) was able to produce aromas from either TP or F4. Using TP as substrate, the produced aromas were 4-oxo-isophorone (1), isophorone (2), cinnamic aldehyde (6), 3-hydroxy-β-cyclocitral (7), safranal (8), geranyl (9), 3-oxo-α-ionone (10), 3-oxo-α-ionol (11), 3-oxo-7,8-dihydro-α-ionone (12), and eugenol (13). Of these aromas, only seven were produced from F4: (1), (2), (7), (8), (10), (11), and (12). In both cases, safranal was the main degradation product (30%). The enzymatic activity responsible for this effect was found in the cytosolic fraction and detected only when S. cerevisiae was grown in the presence of TP or F4.

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Acknowledgements

This research was financially supported by the Consejo Nacional de Ciencia y tecnología (CONACYT), Simorelos program (grant 1990305012), and Consejo estatal de ciencia y Tecnología de Jalisco (COECYTJAL, grant 1811). We thank Gabriela Maldonado-Robledo, Eduardo Rodríguez-Bustamante, Mirna Estarrón-Espinoza for their technical assistance in the realization of this work and Hector Escalona-Buendia and Peter Knauth for their help with the text.

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Authors and Affiliations

  1. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Av. Normalistas 800, Colinas de la Normal, 44270, Guadalajara Jalisco, Mexico

    L. Del Toro-Sánchez, S. Villanueva & E. Lugo-Cervantes

  2. Departamento de Biología Molecular y Biotecnología del Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, 04510, Mexico, DF, Mexico

    L. Del Toro-Sánchez, S. Sánchez & M. A. Ortiz

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  1. L. Del Toro-Sánchez
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  2. S. Sánchez
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  3. M. A. Ortiz
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  4. S. Villanueva
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  5. E. Lugo-Cervantes
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Correspondence to E. Lugo-Cervantes.

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Del Toro-Sánchez, L., Sánchez, S., Ortiz, M.A. et al. Generation of aroma compounds from Ditaxis heterantha by Saccharomyces cerevisiae . Appl Microbiol Biotechnol 72, 155–162 (2006). https://doi.org/10.1007/s00253-005-0244-y

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  • DOI: https://doi.org/10.1007/s00253-005-0244-y

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