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Efficient production of 2-pyrone 4,6-dicarboxylic acid as a novel polymer-based material from protocatechuate by microbial function

  • Biotechnological Products and Process Engineering
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Abstract

Sphingomonas paucimobilis SYK-6, which can degrade various low molecular weight compounds derived from plant polyphenols such as lignin, lignan, and tannin, metabolizes these substances via 2-pyrone-4,6-dicarboxylic acid (PDC). We focused on this metabolic intermediate as a potential raw material for novel, bio-based polymers. We cloned the ligAB and ligC genes of SYK-6, which respectively encode protocatechuate 4,5-dioxygenase and 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase, into a broad host range plasmid vector, pKT230MC. The resulting plasmid, pDVABC, was introduced into the PpY1100 strain of Pseudomonas putida, and we found that PDC could be stably produced from protocatechuate and accumulated. In addition, we examined the efficiency of production of PDC from protocatechuate on a 5-L scale in a Luria–Bertani medium containing 100 mM glucose and determined that PDC was stably produced from protocatechuate to yield 10 g/L or more.

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Authors and Affiliations

  1. Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan

    Yuichiro Otsuka, Masaya Nakamura & Seiji Ohara

  2. Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, 184-8588, Tokyo, Japan

    Kiyotaka Shigehara, Kosuke Sugimura & Yoshihiro Katayama

  3. Nagaoka University of Technology, Nagaoka, Niigata, Japan

    Eiji Masai

Authors
  1. Yuichiro Otsuka
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  2. Masaya Nakamura
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  3. Kiyotaka Shigehara
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  4. Kosuke Sugimura
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  5. Eiji Masai
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  6. Seiji Ohara
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  7. Yoshihiro Katayama
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Correspondence to Yoshihiro Katayama.

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Otsuka, Y., Nakamura, M., Shigehara, K. et al. Efficient production of 2-pyrone 4,6-dicarboxylic acid as a novel polymer-based material from protocatechuate by microbial function. Appl Microbiol Biotechnol 71, 608–614 (2006). https://doi.org/10.1007/s00253-005-0203-7

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  • DOI: https://doi.org/10.1007/s00253-005-0203-7

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