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Xylitol production by a Pichia stipitis D-xylulokinase mutant

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Abstract

Xylitol production by Pichia stipitis FPL-YS30, a xyl3-Δ1 mutant that metabolizes xylose using an alternative metabolic pathway, was investigated under aerobic and oxygen-limited culture conditions. Under both culture conditions, FPL-YS30 (xyl3-Δ1) produced a negligible amount of ethanol and converted xylose mainly into xylitol with comparable yields (0.30 and 0.27 g xylitol/g xylose). However, xylose consumption increased five-fold under aerobic compared to oxygen-limited conditions. This suggests that the efficiency of the alternative route of xylose assimilation is affected by respiration. As a result, the FPL-YS30 strain produced 26 g/l of xylitol, and exhibited a higher volumetric productivity (0.22 g xylitol l−1 h−1) under aerobic conditions.

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Acknowledgements

The authors thank Hal Alper for reading the manuscript critically. This research was supported by NRI/CSREES, grant number 2001-35504-10695.

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Author notes

  1. Thomas W. Jeffries

    Present address: USDA Forest Service, Forest Products Laboratory, One Gifford Pinchot Drive, Madison, WI, 53726-2398, USA

  2. Yong-Su Jin

    Present address: Present address: Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56-422, 77 Massachusetts Ave, Cambridge, MA, 02139, USA

Authors and Affiliations

  1. Department of Food Science, University of Wisconsin-Madison, 1550 Linden Drive, Madison, WI, 53706, USA

    Yong-Su Jin

  2. Department of Chemical Engineering, University of Vigo, As Lagoas, 32004, Ourense, Spain

    Jose Cruz

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  1. Yong-Su Jin
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  2. Jose Cruz
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  3. Thomas W. Jeffries
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Correspondence to Thomas W. Jeffries.

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Jin, YS., Cruz, J. & Jeffries, T. Xylitol production by a Pichia stipitis D-xylulokinase mutant. Appl Microbiol Biotechnol 68, 42–45 (2005). https://doi.org/10.1007/s00253-004-1854-5

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  • DOI: https://doi.org/10.1007/s00253-004-1854-5

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