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Differential regulation and xenobiotic induction of tandem P450 monooxygenase genes pc-1 (CYP63A1) and pc-2 (CYP63A2) in the white-rot fungus Phanerochaete chrysosporium

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Abstract

The two tandem P450 monooxygenase genes (pc-1 and pc-2) reported by us earlier in Phanerochaete chrysosporium were investigated for their regulation under nutrient-limited and nutrient-rich culture conditions. Transcript analysis based on real-time quantitative RT-PCR showed higher expression of pc-1 in defined low-nitrogen and pc-2 in defined high-nitrogen media, with maximum expression on day 4, indicating that the two genes, though tandemly linked, are regulated in a non-coordinate manner. Transcript levels of pc-1 and pc-2 were differentially influenced by the type of carbon source, incubation temperature, and oxygenation. Both genes were inducible by organic xenobiotic chemicals. Of the 42 xenobiotics tested in nutrient-rich cultures, pc-1 transcription was induced 2.12(±0.40)-fold to 6.27(±0.48)-fold in the presence of 19 compounds and pc-2 transcription was induced 2.00(±0.73)-fold to 29.39(±9.40)-fold in the presence of 22 compounds. Particularly, it is significant that both pc-1 and pc-2 are induced by polycyclic aromatic hydrocarbons (PAHs) of varying ring size, including naphthalene (4.35±0.09, 6.02±1.39), phenanthrene (2.82±0.12, 2.14±0.61), pyrene (3.93±0.01, 1.0±0.12), benzanthracene (1.67±0.03, 6.08±1.50), and benzo(a)pyrene (1.55±0.01, 5.54±2.75) respectively. This study constitutes the first report on the identification of P450 genes in a fungus that are responsive to environmentally significant pollutant chemicals (PAHs, DDT, long-chain alkyl phenols) and lignin derivatives.

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Acknowledgement

This work was supported by the NIH’s National Institute of Environmental Health Sciences grant R01-ES10210 (to J.S.Y.).

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  1. Molecular Toxicology Division, Department of Environmental Health, University of Cincinnati, College of Medicine, Cincinnati, OH 45267-0056, USA

    Harshavardhan Doddapaneni & Jagjit S. Yadav

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  1. Harshavardhan Doddapaneni
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  2. Jagjit S. Yadav
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Correspondence to Jagjit S. Yadav.

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Doddapaneni, H., Yadav, J.S. Differential regulation and xenobiotic induction of tandem P450 monooxygenase genes pc-1 (CYP63A1) and pc-2 (CYP63A2) in the white-rot fungus Phanerochaete chrysosporium. Appl Microbiol Biotechnol 65, 559–565 (2004). https://doi.org/10.1007/s00253-004-1645-z

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  • DOI: https://doi.org/10.1007/s00253-004-1645-z

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