Abstract
The Aspergillus nidulans gene xlnA coding for the fungal xylanase X22 has been cloned and expressed in two heterologous bacterial hosts: Streptomyces lividans and Brevibacterium lactofermentum. Streptomyces strains yielded 10 units/ml of xylanase when the protein was produced with its own signal peptide, and 19 units/ml when its signal peptide was replaced by the one for xylanase Xys1 from Streptomyces halstedii. B. lactofermentum was also able to produce xylanase X22, affording 6 units/ml upon using either the Aspergillus xlnA signal peptide or Streptomyces xysA. These production values are higher than those previously reported for the heterologous expression of the A. nidulans xlnA gene in Saccharomyces cerevisiae (1 unit/ml). Moreover, the X22 enzyme produced by Streptomyces lividans showed oenological properties, indicating that this Streptomyces recombinant strain is a good candidate for the production of this enzyme at the industrial scale.
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Acknowledgements
This research was supported by a grant from the European Union (FD1997-1134-C03). We thank R. Valle for her excellent technical work. Drs. J.M. Férnandez-Abalos and F. Leal are thanked for their comments. Thanks are also due to N. Skinner for supervising the English version of the manuscript. These experiments comply with the current laws in Spain.
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Díaz, M., Adham, S.A.I., Ramón, D. et al. Streptomyces lividans and Brevibacterium lactofermentum as heterologous hosts for the production of X22 xylanase from Aspergillus nidulans . Appl Microbiol Biotechnol 65, 401–406 (2004). https://doi.org/10.1007/s00253-004-1633-3
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DOI: https://doi.org/10.1007/s00253-004-1633-3