Skip to main content
Log in

Styrene degradation by Pseudomonas sp. SR-5 in biofilters with organic and inorganic packing materials

  • Environmental Biotechnology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Pseudomonas sp. SR-5 was isolated as a styrene-degrading bacterium. In liquid culture containing 1% (v/v) styrene, more than 90% styrene was degraded in 53 h and the doubling time of SR-5 was 2 h. The removal of styrene gas was investigated in biofilters for 31 days using an organic packing material of peat and an inorganic packing material of ceramic inoculated with SR-5. The maximum-styrene-elimination capacities for peat and ceramic packing materials were 236 and 81 g m−3 h−1, respectively. The percentage of styrene converted to low molecular weight compounds including CO2 in the peat and ceramic biofilters during a 10-day operation were estimated to be 90.4 and 36.7%, respectively. As the pressure drop in the peat bioflter at the end of experiment was significantly higher than that in ceramic biofilter, a biofilter using a mixture of peat and ceramic was tested. We determined that the maximum elimination capacity was 170 g m−3 h−1 and the production of low molecular weight compounds was 95% at a low pressure drop for this mixed packing material filter.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Arnold M, Reittu A, von Wright A, Martikainen PJ (1997) Bacterial degradation of styrene in waste gases using a peat filtration. Appl Microbiol Biotechnol 48:738–744

    Article  CAS  PubMed  Google Scholar 

  • Baggi G, Boga MM, Catelani D, Galli E, Treccani V (1983) Styrene catabolism by a strain of Pseudomonas fluorescens. Syst Appl Microbiol 4:141–147

    CAS  Google Scholar 

  • Cox HHJ (1995) Styrene removal from waste gas by the fungus Exophiala jeanselmei in a biofilter. PhD thesis, University of Groningen, Netherlands

  • Cox HHJ, Houtman JHM, Doddema HJ, Harder W (1993) Enrichment of fungi and degradation of styrene in biofilters. Biotechnol Lett 15:737–742

    CAS  Google Scholar 

  • Cox HHJ, Faber BW, van Heiningen WNM, Radhoe H, Doddema HJ, Harder W (1996a) Styrene metabolism in Exophiala jeanselmei and involvement of a cytochrome P-450-dependent styrene monooxygenase. Appl Environ Microbiol 62:1471–1474

    CAS  PubMed  Google Scholar 

  • Cox HHJ, Magielsen FJ, Doddema HJ, Harber W (1996b) Influence of the water content and water activity on styrene degradation by Exophiala jeanselmei in biofilters. Appl Microbiol Biotechnol 45:851–856

    Article  CAS  Google Scholar 

  • Cox HHJ, Moerman RE, van Baalen S, van Heiningen WNM, Doddema HJ, Harder W (1997) Performance of a styrene-degrading biofilter containing the yeast Exophiala jeanselmei. Biotechnol Bioeng 53:259–266

    CAS  Google Scholar 

  • Hartmans S, Smits JP, van der Werf MJ, Volkering F, de Bont JAM (1989) Metabolism of styrene oxide and 2-phenylethanol in the styrene-degradation Xanthobacter strain 124X. Appl Environ Microbiol 55:2850–2855

    CAS  Google Scholar 

  • Hirai M, Kamamoto M, Yani M, Shoda M (2001) Comparison of the biological H2S removal characteristics among four inorganic packing materials. J Biosci Bioeng 91:396–402

    Article  CAS  Google Scholar 

  • Itoh N, Yoshida K, Okada K (1996) Isolation and identification of styrene-degrading Corynebacterium strains, and their styrene metabolism. Biosci Biotechnol Biochem 60:1826–1830

    CAS  PubMed  Google Scholar 

  • Itoh N, Morihama R, Wang J, Okada K, Mizuguchi N (1997) Purification and characterization of phenylacetaldehyde reductase from a styrene-assimilation Corynebacterium strain, ST-10. Appl Environ Microbiol 63:3783–3788

    CAS  PubMed  Google Scholar 

  • Lee K, Gibson DT (1996) Stereospecific dehydroxylation of the styrene vinyl group by purified naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4. J Bacteriol 178:3353–3356

    CAS  PubMed  Google Scholar 

  • Miller RR, Newhook R, Poole A (1994) Styrene production, use, and human exposure. Crit Rev Toxicol 24:1–10

    PubMed  Google Scholar 

  • Morales M, Revah S, Auria R (1998) Start-up and the effect of gases ammonia addition on a biofilter for the elimination of toluene vapors. Biotechnol Bioeng 60:483–491

    CAS  PubMed  Google Scholar 

  • Okamoto K, Izawa M, Yanase H (2003) Isolation and application of a styrene-degradation strain of Pseudomonas putida to biofiltration. J Biosci Bioeng 95:633–636

    CAS  Google Scholar 

  • Ortiz I, Revah S, Auria R (2001) Effect of packing material on the biofiltration of benzene, toluene and xylene vapors. Environ Technol 24:265–275

    Google Scholar 

  • O’Connor K, Buckley CM, Hartmans S, Dobson ADW (1995) Possible regulatory role for nonaromatic carbon sources in styrene degradation by Pseudomonas putida CA-3. Appl Environ Microbiol 61:544–548

    CAS  PubMed  Google Scholar 

  • O’Connor K, Duetz W, Wind B, Dobson ADW (1996) The effect of nutrient limitation of styrene metabolism in Pseudomonas putida CA-3. Appl Environ Microbiol 62:3594–3599

    Google Scholar 

  • Paca J, Koutsky B, Maryska M, Halecky M (2001) Styrene degradation along the bed height of perlite biofilter. J Chem Technol Biotechnol 76:873–878

    Article  CAS  Google Scholar 

  • Rho D, Matte N, Utiger M (1998) Biodegradation dynamics of styrene and acetone from waste gases in a biotrickling filter. In: Proceedings of the USC-TRG conference on biofiltration. pp 15–23

  • Sorial GA, Smith FL, Suidan MT, Pandit A, Biswas P, Brenner RC (1998) Evaluation of trickle-bed air biofilter performance for styrene removal. Water Res 32:1593–1603

    CAS  Google Scholar 

  • Teranperez W, Domenech F, Roger P, Christen P (2002) Effect of mineral salts addition on the behaviour of an ethanol biofilter. Environ Technol 23:981–988

    CAS  PubMed  Google Scholar 

  • Warhurst AM, Clarke KF, Hill RA, Holt RA, Fewson CA (1994) Metabolism of Rhodococcus rodochrous NCIMB 13259. Appl Environ Microbiol 60:1137–1145

    CAS  PubMed  Google Scholar 

  • Yoon IK, Park CH (2002) Effects of gas flow rate, inlet concentration and temperature on biofiltration of volatile organic compounds in a peat-packed biofilter. J Biosci Bioeng 93:165–169

    Article  CAS  Google Scholar 

  • Zilli M, Converti A, Lodi A, Broughi MD, Ferraiolo G (1993) Phenol removal from waste gases with a biological filter by Pseudomonas putida. Biotechnol Bioeng 41:693–699

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. Shoda.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jang, J.H., Hirai, M. & Shoda, M. Styrene degradation by Pseudomonas sp. SR-5 in biofilters with organic and inorganic packing materials. Appl Microbiol Biotechnol 65, 349–355 (2004). https://doi.org/10.1007/s00253-004-1628-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-004-1628-0

Keywords

Navigation