Abstract
The genes encoding two conjugated polyketone reductases (CPR-C1, CPR-C2) of Candida parapsilosis IFO 0708 were cloned and sequenced. The genes encoded a total of 304 and 307 amino acid residues for CPR-C1 and CPR-C2, respectively. The deduced amino acid sequences of the two enzymes showed high similarity to each other and to several proteins of the aldo-keto reductase (AKR) superfamily. However, several amino acid residues in putative active sites of AKRs were not conserved in CPR-C1 and CPR-C2. The two CPR genes were overexpressed in Escherichia coli. The E. coli transformant bearing the CPR-C2 gene almost stoichiometrically reduced 30 mg ketopantoyl lactone/ml to d-pantoyl lactone.
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Acknowledgements
This work was supported in part by a Grant-in-Aid for Scientific Research (No. 13853009, to S.S.) from the Japan Society for the Promotion of Science. This work was also carried out as a part of the Project for Development of a Technological Infrastructure for Industrial Bioprocesses on R&D of New Industrial Science and Technology Frontiers by the Ministry of Economy, Trade & Industry (METI) and entrusted by the New Energy and Industrial Technology Development Organization (NEDO) (to S.S.).
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Kataoka, M., Delacruz-Hidalgo, AR.G., Akond, M.A. et al. Gene cloning and overexpression of two conjugated polyketone reductases, novel aldo-keto reductase family enzymes, of Candida parapsilosis . Appl Microbiol Biotechnol 64, 359–366 (2004). https://doi.org/10.1007/s00253-003-1484-3
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DOI: https://doi.org/10.1007/s00253-003-1484-3