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SulA-independent filamentation of Escherichia coli during growth after release from high hydrostatic pressure treatment

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Abstract

To improve the efficiency of sterilization by high hydrostatic pressure treatment (HPT), it is desirable to know the biochemical process of bacteria most sensitive to the treatment. We investigated growth properties after release from HPT of exponentially growing Escherichia coli K-12 cells. We observed growth retardation after treatment (30 min at 37°C) above 75 MPa. Long filamentous cells of about eight times normal cell length were observed at 90 min growth after treatment at 75 MPa. In the subsequent period the filamentous cells divided into normal-sized cells. recA and sulA mutant strains also formed filamentous cells, indicating that filamentation was SulA-independent. Nucleoids segregated normally in the filamentous cells. Only one FtsZ ring (or none) was detected at possible division sites in the elongated cells. Western blotting analysis demonstrated that the amount of FtsZ protein was not affected by the treatment. GTP-dependent in vitro polymerization of either FtsZ protein in E. coli crude extract or purified FtsZ protein, however, was sensitive to HPT. These facts suggest that HPT at 75 MPa denatures a fraction of FtsZ molecules, and that these denatured molecules interfere with the polymerization of functional FtsZ, resulting in the significantly reduced number of FtsZ rings.

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Acknowledgements

This work was supported by a Grant-in-Aid for Scientific Research (C) (No. 1266086) from The Ministry of Education, Culture, Sports, Science and Technology, Japan to M.Y. and Sasakawa Scientific Research Grant (No. 14–246) from The Japan Science Society to T.K.

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Authors and Affiliations

  1. Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252–8510, Japan

    T. Kawarai, S. Furukawa, K. Suzuki, H. Ogihara & M. Yamasaki

  2. Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Kanagawa 226–8501, Japan

    M. Wachi & H. Ogino

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  1. T. Kawarai
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  2. M. Wachi
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  3. H. Ogino
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  4. S. Furukawa
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  5. K. Suzuki
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  6. H. Ogihara
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  7. M. Yamasaki
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Correspondence to M. Yamasaki.

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Kawarai, T., Wachi, M., Ogino, H. et al. SulA-independent filamentation of Escherichia coli during growth after release from high hydrostatic pressure treatment. Appl Microbiol Biotechnol 64, 255–262 (2004). https://doi.org/10.1007/s00253-003-1465-6

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  • DOI: https://doi.org/10.1007/s00253-003-1465-6

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